scholarly journals Effect of inorganic phosphate on serum ionized calcium concentration in vitro: A reassessment of the “trade-off hypothesis”

1985 ◽  
Vol 28 (6) ◽  
pp. 932-935 ◽  
Author(s):  
Andrew J. Adler ◽  
Nereida Ferran ◽  
Geoffrey M. Berlyne
Keyword(s):  
Inorganic Phosphate ◽  
Calcium Concentration ◽  
Ionized Calcium ◽  
Trade Off ◽  
Serum Ionized Calcium

The Effect of the Alkaline Tide on Serum-Ionized Calcium Concentration in Man

Digestion ◽  
1977 ◽  
Vol 15 (3) ◽  
pp. 175-181 ◽  
Author(s):  
W. Hughes ◽  
S. Cohen ◽  
D. Arvan ◽  
B. Seamonds
Keyword(s):  
Calcium Concentration ◽  
Ionized Calcium ◽  
Alkaline Tide ◽  
Serum Ionized Calcium

Measurement of Serum Ionized Calcium with the Ion-Exchange Electrode

1972 ◽  
Vol 18 (12) ◽  
pp. 1459-1462 ◽  
Author(s):  
V L Subryan ◽  
M M Popovtzer ◽  
S D Parks ◽  
E B Reeve
Keyword(s):  
Ion Exchange ◽  
Reproducibility Of Results ◽  
Room Temperature ◽  
Calcium Concentration ◽  
Ionized Calcium ◽  
Control Solution ◽  
Electrode Response ◽  
Serum Ionized Calcium ◽  
Evacuated Tubes

Abstract We found no differences in pH or ionized calcium concentration of serum from blood either collected into evacuated tubes or collected with syringes and separated under oil. Sera can be stored frozen in 1-ml plastic insulin-type syringes for one to three days without significant changes in pH or ionized calcium concentration, whereas storage under oil with or without re-equilibration with CO2 is associated with significant changes in both. Standards prepared with trypsin and triethanolamine give faster electrode response, and their ionized calcium concentrations remain unchanged for 6 h at room temperature. We suggest a not entirely satisfactory control solution of buffered protein, for use in assessing reproducibility of results.

Increase in Serum Ionized Calcium during Exercise

1977 ◽  
Vol 53 (6) ◽  
pp. 579-586 ◽  
Author(s):  
S. Pors Nielsen ◽  
T. Falch Christiansen ◽  
O. Hartling ◽  
J. Trap-Jensen
Keyword(s):  
Blood Lactate ◽  
Venous Blood ◽  
Recovery Period ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Normal Subjects ◽  
Ionized Calcium ◽  
Serum Ionized Calcium

1. Normal subjects showed an average increase in serum ionized calcium (Ca2+) concentration of 0·11 mmol/l in peripheral venous blood 10 min after onset of bicycle exercise at 70% of maximum aerobic capacity. The corresponding mean rise in serum total calcium concentration was 0·21 mmol/l. 2. The change in serum Ca2+ as result of acidification was studied in 20 normal subjects by carbon dioxide equilibration in vitro followed by measurement of serum Ca2+. The log serum Ca2+ was inversely proportional to serum pH. 3. The Δlog serum Ca2+/ΔpH in vitro was similar to the Δlog serum Caa+/ΔpH in vivo during exercise, this ratio, however, being somewhat greater during the first minute of exercise. 4. Serum Ca2+ returned to normal values about 20 min after stopping exercise as the pH returned to normal, but the fall immediately after stopping exercise was more pronounced than that due to the change in pH, as predicted from the studies in vitro. 5. Blood lactate concentration rose from 0·86 to 8·41 mmol/l after 10 min exercise, but the rise in blood lactate during exercise was slower than the rise in serum Ca2+. Also the fall during the recovery period was delayed compared with the fall in serum Ca2+. 6. It is suggested that the rise in serum Ca2+ during severe muscular exercise might be important for the physiological adaptations during work, and for bone metabolism.

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