scholarly journals Influence of glomerular filtration rate on the rate of para-aminohippurate secretion by the rat kidney: Micropuncture and clearance studies

1975 ◽  
Vol 7 (6) ◽  
pp. 385-396 ◽  
Author(s):  
Dieter Häberle ◽  
A. Ober ◽  
G. Ruhland
Keyword(s):  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
Rat Kidney ◽  
Clearance Studies ◽  
Kidney Micropuncture

Lithium clearance method and the renal response to low-dose dopamine in man: a randomized, controlled study

1993 ◽  
Vol 84 (2) ◽  
pp. 237-242 ◽  
Author(s):  
Niels Vidiendal Olsen ◽  
Michael Hecht Olsen ◽  
Niels Fogh-Andersen ◽  
Bo Feldt-Rasmussen ◽  
Annelise Kamper ◽  
...  
Keyword(s):  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Plasma Flow ◽  
Filtration Rate ◽  
Low Dose ◽  
Renal Plasma Flow ◽  
Effective Renal Plasma Flow ◽  
Lithium Clearance ◽  
Clearance Studies ◽  
Sodium Clearance

1. The effect of a single dose of lithium on renal function before and during intravenous infusion of dopamine (3 μg min−1 kg−1) was investigated in 12 healthy males. In a double-blind and randomized design, 450 mg or 600 mg of lithium carbonate or placebo was administered orally at 22.00 hours on three different occasions. After an overnight fast, the subjects were water-loaded and clearance studies were started at 09.00 hours with a 1 h baseline period and three 1 h periods during dopamine infusion. 2. Baseline sodium clearance with placebo was 0.65 ± 0.35 ml/min, but with lithium it increased to 1.25 ± 0.44 (P < 0.001) and 1.17 ± 0.46 ml/min (P < 0.01) after 450 and 600 mg, respectively. Urine flow rates were unchanged compared with placebo. Lithium did not significantly affect glomerular filtration rate, but both doses slightly increased effective renal plasma flow by 7% (P < 0.05) and 10% (P < 0.01), respectively. 3. The maximal natriuretic and diuretic effects of dopamine were not reduced by lithium, but the percentage increases in sodium clearance were significantly diminished after 450 mg (P < 0.01) and 600 mg (P < 0.001) of lithium. Lithium had no effect on dopamine-induced changes in effective renal plasma flow, glomerular filtration rate or osmolal clearance. Neither lithium nor dopamine influenced plasma concentrations of renin, aldosterone or atrial natriuretic peptide. 4. In conclusion, single test doses of lithium, as normally used in lithium clearance studies, increase baseline values of sodium clearance and effective renal plasma flow. Although these effects of lithium do not reduce the maximal renal responses to low-dose dopamine, they result in an underestimation of the percentage increase in sodium excretion.

Influence of Contrast Media on Single Nephron Glomerular Filtration Rate in Rat Kidney: A Comparison between Diatrizoate, Iohexol, Ioxaglate, and Iotrolan

1992 ◽  
Vol 33 (6) ◽  
pp. 596-599
Author(s):  
J. Ueda ◽  
A. Nygren ◽  
P. Hansell ◽  
U. Erikson
Keyword(s):  
Glomerular Filtration Rate ◽  
Contrast Media ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
Rat Kidney ◽  
Control Value ◽  
Osmotic Effect ◽  
Micropuncture Technique ◽  
Single Nephron

The effects of slow (10 min) i.v. infusions of contrast media (CM, 1 600 mg I/kg b.w.) on single nephron glomerular filtration rate (SNGFR) in the rat kidney were investigated using a micropuncture technique. Diatrizoate, iohexol, or ioxaglate did not change SNGFR, although a tendency towards a transient suppression was seen during the infusion phase. Iotrolan infusion, however, decreased SNGFR (p < 0.05) and the value still remained below the control value 25 min after the start of infusion. Iotrolan is a nonionic dimeric CM and has a lower osmotic effect in the tubules than the ionic dimeric CM and the monomeric CM when given in iodine equivalent doses. These characteristics of iotrolan have probably some influence on the depression of SNGFR after iotrolan injection.

scholarly journals Rapid diagnosis and quantification of acute kidney injury using fluorescent ratio-metric determination of glomerular filtration rate in the rat

2010 ◽  
Vol 299 (5) ◽  
pp. F1048-F1055 ◽  
Author(s):  
Exing Wang ◽  
Ruben M. Sandoval ◽  
Silvia B. Campos ◽  
Bruce A. Molitoris
Keyword(s):  
Acute Kidney Injury ◽  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Filtration Rate ◽  
Fluorescence Probe ◽  
Rat Kidney ◽  
Kidney Injury ◽  
Rapid Diagnosis ◽  
Fluorescent Intensity ◽  
Two Photon

The rapid diagnosis and quantification of acute kidney injury (AKI) severity remain high clinical priorities. By combining intravital fluorescent ratiometric two-photon kidney imaging and the two-compartment pharmacokinetics model, we demonstrate that rapid quantification of glomerular filtration rate (GFR) can be achieved in physiologic and AKI rat kidney models. Using a bolus infusion of a mixture of FITC-inulin and a 500-kDa Texas Red dextran, a full spectrum of GFR values, ranging from 0.17 to 1.12 ml·min−1·100 g−1, was obtained. The GFR values thus determined correlated well with values obtained by the standard 2-h inulin infusion clearance method with a Pearson's correlation coefficient of 0.85. In addition, postischemia deterioration was studied by measuring GFR using the two-photon approach during 24 h following a 45-min bilateral ischemia clamp model. The GFR was found to decline sharply during the initial 4 h followed by a nadir with little sign of rising over the ensuing 24-h period. Moreover, a FITC-labeled 5-kDa dextran was identified as having nearly identical filtration characteristics as FITC-inulin, but had markedly increased fluorescent intensity, thus minimizing the quantity needed for individual studies. The technique reported allows for very rapid GFR determinations, within 10–15 min, based on plasma clearance of a freely filtered fluorescence probe, instead of a prolonged one-compartment interstitial space reporter molecule clearance employed by other technologies.

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