scholarly journals Elastin Modification by 4-Hydroxynonenal in Hairless Mice Exposed to UV-A. Role in Photoaging and Actinic Elastosis

2015 ◽  
Vol 135 (7) ◽  
pp. 1873-1881 ◽  
Author(s):  
Pauline Larroque-Cardoso ◽  
Caroline Camaré ◽  
Florence Nadal-Wollbold ◽  
Marie- Hélène Grazide ◽  
Mélanie Pucelle ◽  
...  
Keyword(s):  
Hairless Mice ◽  
Actinic Elastosis

scholarly journals 4-Hydroxynonenal Contributes to Fibroblast Senescence in Skin Photoaging Evoked by UV-A Radiation

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365 ◽  
Author(s):  
Audrey Swiader ◽  
Caroline Camaré ◽  
Paul Guerby ◽  
Robert Salvayre ◽  
Anne Negre-Salvayre
Keyword(s):  
Lipid Peroxidation ◽  
Dermal Fibroblasts ◽  
Connective Tissues ◽  
Hairless Mice ◽  
Cultured Fibroblasts ◽  
Γh2ax Foci ◽  
Skin Photoaging ◽  
Actinic Elastosis

Solar ultraviolet A (UV-A) radiation promotes a huge variety of damages on connective tissues and dermal fibroblasts, including cellular senescence, a major contributor of skin photoaging. The mechanisms of skin photoaging evoked by UV-A partly involve the generation of reactive oxygen species and lipid peroxidation. We previously reported that 4-hydroxynonenal (HNE), a lipid peroxidation-derived aldehyde, forms adducts on elastin in the skins of UV-A irradiated hairless mice, possibly contributing to actinic elastosis. In the present study, we investigated whether and how HNE promotes fibroblast senescence in skin photoaging. Dermal fibroblasts of skins from UV-A-exposed hairless mice exhibited an increased number of γH2AX foci characteristic of cell senescence, together with an accumulation of HNE adducts partly colocalizing with the cytoskeletal protein vimentin. Murine fibroblasts exposed to UV-A radiation (two cycles of 15 J/cm2), or HNE (30 µM, 4 h), exhibited senescence patterns characterized by an increased γH2AX foci expression, an accumulation of acetylated proteins, and a decreased expression of the sirtuin SIRT1. HNE adducts were detected on vimentin in cultured fibroblasts irradiated by UV-A or incubated with HNE. The HNE scavenger carnosine prevented both vimentin modification and fibroblast senescence evoked by HNE in vitro and in the skins of UV-A-exposed mice. Altogether, these data emphasize the role of HNE and lipid peroxidation-derived aldehydes in fibroblast senescence, and confirm the protective effect of carnosine in skin photoaging.

Advantages of Using Hairless Mice Versus Haired Mice to Test Sunscreen Efficacy Against Photoimmune Suppression¶

2003 ◽  
Vol 78 (1) ◽  
pp. 37 ◽  
Author(s):  
Tae-Heung Kim ◽  
Honnavara N. Ananthaswamy ◽  
Margaret L. Kripke ◽  
Stephen E. Ullrich
Keyword(s):  
Hairless Mice

scholarly journals Age-related decline in the taurine content of the skin in rodents

Amino Acids ◽  
2021 ◽  
Author(s):  
Tomohisa Yoshimura ◽  
Yuki Inokuchi ◽  
Chikako Mutou ◽  
Takanobu Sakurai ◽  
Tohru Nagahama ◽  
...  
Keyword(s):  
High Performance ◽  
Age Groups ◽  
Immunohistochemical Analysis ◽  
Sprague Dawley ◽  
Taurine Supplementation ◽  
Hairless Mice ◽  
Age Related ◽  
Sprague Dawley Rats ◽  
High Concentrations ◽  
Effects Of Aging

AbstractTaurine, a sulfur-containing amino acid, occurs at high concentrations in the skin, and plays a role in maintaining the homeostasis of the skin. We investigated the effects of aging on the content and localization of taurine in the skin of mice and rats. Taurine was extracted from the skin samples of hairless mice and Sprague Dawley rats, and the taurine content of the skin was determined by high-performance liquid chromatography (HPLC). The results of the investigation revealed that the taurine content in both the dermis and epidermis of hairless mice declined significantly with age. Similar age-related decline in the skin taurine content was also observed in rats. In contrast, the taurine content in the sole remained unchanged with age. An immunohistochemical analysis also revealed a decreased skin taurine content in aged animals compared with younger animals, although no significant differences in the localization of taurine were observed between the two age groups. Supplementation of the drinking water of aged mice with 3% (w/v) taurine for 4 weeks increased the taurine content of the epidermis, but not the dermis. The present study showed for the first time that the taurine content of the skin decreased with age in mice and rats, which may be related to the impairment of the skin homeostasis observed with aging. The decreased taurine content of the epidermis in aged animals was able to be rescued by taurine supplementation.

scholarly journals Ginsenosides repair UVB-induced skin barrier damage in BALB/c hairless mice and HaCaT keratinocytes

2021 ◽  
Author(s):  
Zhenzhuo Li ◽  
Rui Jiang ◽  
Manying Wang ◽  
Lu Zhai ◽  
Jianzeng Liu ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Hacat Keratinocytes ◽  
Hairless Mice

Silibinin Inhibits Inflammatory and Angiogenic Attributes in Photocarcinogenesis in SKH-1 Hairless Mice

2007 ◽  
Vol 67 (7) ◽  
pp. 3483-3491 ◽  
Author(s):  
Mallikarjuna Gu ◽  
Rana P. Singh ◽  
Sivanandhan Dhanalakshmi ◽  
Chapla Agarwal ◽  
Rajesh Agarwal
Keyword(s):  
Hairless Mice
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