scholarly journals Knockdown of Filaggrin in a Three-Dimensional Reconstructed Human Epidermis Impairs Keratinocyte Differentiation

2014 ◽  
Vol 134 (12) ◽  
pp. 2938-2946 ◽  
Author(s):  
Valérie Pendaries ◽  
Jeremy Malaisse ◽  
Laurence Pellerin ◽  
Marina Le Lamer ◽  
Rachida Nachat ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Human Epidermis ◽  
Keratinocyte Differentiation ◽  
Reconstructed Human Epidermis

scholarly journals In a three-dimensional reconstructed human epidermis filaggrin-2 is essential for proper cornification

2015 ◽  
Vol 6 (2) ◽  
pp. e1656-e1656 ◽  
Author(s):  
V Pendaries ◽  
M Le Lamer ◽  
L Cau ◽  
B Hansmann ◽  
J Malaisse ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

10.3791/61802 ◽  
2021 ◽  
Author(s):  
Irini M. Dijkhoff ◽  
Benedetta Petracca ◽  
Roxane Prieux ◽  
Giuseppe Valacchi ◽  
Barbara Rothen-Rutishauser ◽  
...  
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Human Epidermis ◽  
Reconstructed Human Epidermis

scholarly journals Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2528
Author(s):  
Denisse Esther Mallaupoma Camarena ◽  
Larissa Satiko Alcântara Sekimoto Matsuyama ◽  
Silvya Stuchi Maria-Engler ◽  
Luiz Henrique Catalani
Keyword(s):  
Three Dimensional ◽  
Synthetic Polymers ◽  
In Vitro Models ◽  
Human Epidermis ◽  
Polybutylene Terephthalate ◽  
Reconstructed Human Epidermis ◽  
Dermal Irritation ◽  
Thickness Skin ◽  
Cell Growth And Proliferation

The development of products for topical applications requires analyses of their skin effects before they are destined for the market. At present, the ban on animal use in several tests makes the search for in vitro models (such as artificial skin) necessary to characterize the risks involved. In this work, tissue engineering concepts were used to manufacture collagen-free three-dimensional scaffolds for cell growth and proliferation. Two different human skin models—reconstructed human epidermis and full-thickness skin—were developed from electrospun scaffolds using synthetic polymers such as polyethylene terephthalate, polybutylene terephthalate, and nylon 6/6. After the construction of these models, their histology was analyzed by H&E staining and immunohistochemistry. The results revealed a reconstructed epidermal tissue, duly stratified, obtained from the nylon scaffold. In this model, the presence of proteins involved in the epidermis stratification process (cytokeratin 14, cytokeratin 10, involucrin, and loricrin) was confirmed by immunohistochemistry and Western blot analysis. The nylon reconstructed human epidermis model’s applicability was evaluated as a platform to perform irritation and corrosion tests. Our results demonstrated that this model is a promising platform to assess the potential of dermal irritation/corrosion of chemical products.

Implementation, availability and regulatory status of an OECD accepted Reconstructed Human Epidermis model in Brazil

2018 ◽  
Vol 6 (1) ◽  
pp. 64 ◽  
Author(s):  
Rodrigo De Vecchi ◽  
Vanja Dakic ◽  
Guilherme Mattos ◽  
Anne-Sophie Rigaudeau ◽  
Veronica Oliveira ◽  
...  
Keyword(s):  
Human Epidermis ◽  
Reconstructed Human Epidermis ◽  
Regulatory Status

Malassezia colonisation on a reconstructed human epidermis: Imaging studies

Mycoses ◽  
2019 ◽  
Vol 62 (12) ◽  
pp. 1194-1201 ◽  
Author(s):  
Ana Filipa Pedrosa ◽  
Carmen Lisboa ◽  
Joana Branco ◽  
Ana C. Almeida ◽  
Claudia Mendes ◽  
...  
Keyword(s):  
Human Epidermis ◽  
Imaging Studies ◽  
Reconstructed Human Epidermis
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