scholarly journals Transgenic Mouse Technology in Skin Biology: Inducible Gene Knockout in Mice

2014 ◽  
Vol 134 (7) ◽  
pp. 1-4 ◽  
Author(s):  
Christian Günschmann ◽  
Elena Chiticariu ◽  
Bhavuk Garg ◽  
Merve Meliha Hiz ◽  
Yora Mostmans ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Gene Knockout ◽  
Skin Biology ◽  
Inducible Gene

scholarly journals Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

2015 ◽  
Vol 17 (2) ◽  
pp. 233-244 ◽  
Author(s):  
Yuejun Chen ◽  
Jingyuan Cao ◽  
Man Xiong ◽  
Andrew J. Petersen ◽  
Yi Dong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Gene Knockout ◽  
Human Stem Cell ◽  
Stem Cell Lines ◽  
Inducible Gene

scholarly journals Human Desmoglein-2 and Human CD46 Mediate Human Adenovirus Type 55 Infection, but Human Desmoglein-2 Plays the Major Roles

2020 ◽  
Vol 94 (17) ◽  
Author(s):  
Ying Feng ◽  
Changhua Yi ◽  
Xinglong Liu ◽  
Linbing Qu ◽  
Wan Su ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Transgenic Mouse ◽  
Gene Knockout ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Host Cells ◽  
Respiratory Pathogen ◽  
Cellular Receptors ◽  
Fiber Protein ◽  
Mouse Lines

ABSTRACT Human adenovirus type 55 (HAdV55) represents an emerging respiratory pathogen and causes severe pneumonia with high fatality in humans. The cellular receptors, which are essential for understanding the infection and pathogenesis of HAdV55, remain unclear. In this study, we found that HAdV55 binding and infection were sharply reduced by disrupting the interaction of viral fiber protein with human desmoglein-2 (hDSG2) but only slightly reduced by disrupting the interaction of viral fiber protein with human CD46 (hCD46). Loss-of-function studies using soluble receptors, blocking antibodies, RNA interference, and gene knockout demonstrated that hDSG2 predominantly mediated HAdV55 infection. Nonpermissive rodent cells became susceptible to HAdV55 infection when hDSG2 or hCD46 was expressed, but hDSG2 mediated more efficient HAd55 infection than hCD46. We generated two transgenic mouse lines that constitutively express either hDSG2 or hCD46. Although nontransgenic mice were resistant to HAdV55 infection, infection with HAdV55 was significantly increased in hDSG2+/+ mice but was much less increased in hCD46+/+ mice. Our findings demonstrate that both hDSG2 and hCD46 are able to mediate HAdV55 infection but hDSG2 plays the major roles. The hDSG2 transgenic mouse can be used as a rodent model for evaluation of HAdV55 vaccine and therapeutics. IMPORTANCE Human adenovirus type 55 (HAdV55) has recently emerged as a highly virulent respiratory pathogen and has been linked to severe and even fatal pneumonia in immunocompetent adults. However, the cellular receptors mediating the entry of HAdV55 into host cells remain unclear, which hinders the establishment of HAdV55-infected animal models and the development of antiviral approaches. In this study, we demonstrated that human desmoglein-2 (hDSG2) plays the major roles during HAdV55 infection. Human CD46 (hCD46) could also mediate the infection of HAdV55, but the efficiency was much lower than for hDSG2. We generated two transgenic mouse lines that express either hDSG2 or hCD46, both of which enabled HAd55 infection in otherwise nontransgenic mice. hDSG2 transgenic mice enabled more efficient HAdV55 infection than hCD46 transgenic mice. Our study adds to our understanding of HAdV55 infection and provides an animal model for evaluating HAdV55 vaccines and therapeutics.

S.19.03 An inducible gene knockout system targeted to BDNF in the brain

2004 ◽  
Vol 14 ◽  
pp. S148
Author(s):  
L.M. Monteggia ◽  
M. Barrot ◽  
C.M. Powell ◽  
O. Berton ◽  
V. Galanis ◽  
...  
Keyword(s):  
Gene Knockout ◽  
The Brain ◽  
Inducible Gene

scholarly journals Transgenic Mouse Technology in Skin Biology: Generation of Knockin Mice

2014 ◽  
Vol 134 (12) ◽  
pp. 1-3 ◽  
Author(s):  
Frederik Tellkamp ◽  
Farida Benhadou ◽  
Jeroen Bremer ◽  
Maria Gnarra ◽  
Jana Knüver ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Skin Biology

scholarly journals Inducible gene knockout of transcription factor recombination signal binding protein‐J reveals its essential role in T versus B lineage decision

2002 ◽  
Vol 14 (6) ◽  
pp. 637-645 ◽  
Author(s):  
Hua Han ◽  
Kenji Tanigaki ◽  
Norio Yamamoto ◽  
Kazuki Kuroda ◽  
Momoko Yoshimoto ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Essential Role ◽  
Gene Knockout ◽  
Binding Protein ◽  
Recombination Signal ◽  
Lineage Decision ◽  
B Lineage ◽  
Inducible Gene

scholarly journals Transgenic Mouse Technology in Skin Biology: Generation of Complete or Tissue-Specific Knockout Mice

2014 ◽  
Vol 134 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Lukas Scharfenberger ◽  
Tina Hennerici ◽  
Gábor Király ◽  
Sophie Kitzmüller ◽  
Marigje Vernooij ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Knockout Mice ◽  
Tissue Specific ◽  
Skin Biology
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