scholarly journals Merkel Cell Polyomavirus–Positive Merkel Cell Carcinoma Cells Do Not Require Expression of the Viral Small T Antigen

2013 ◽  
Vol 133 (8) ◽  
pp. 2059-2064 ◽  
Author(s):  
Sabrina Angermeyer ◽  
Sonja Hesbacher ◽  
Jürgen C. Becker ◽  
David Schrama ◽  
Roland Houben
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Small T Antigen

scholarly journals Merkel Cell Polyomavirus Small T Antigen Initiates Merkel Cell Carcinoma-like Tumor Development in Mice

2017 ◽  
Vol 77 (12) ◽  
pp. 3151-3157 ◽  
Author(s):  
Monique E. Verhaegen ◽  
Doris Mangelberger ◽  
Paul W. Harms ◽  
Markus Eberl ◽  
Dawn M. Wilbert ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
Tumor Development ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Merkel Cell ◽  
Small T Antigen

scholarly journals Serine 220 phosphorylation of the Merkel cell polyomavirus large T antigen crucially supports growth of Merkel cell carcinoma cells

2015 ◽  
Vol 138 (5) ◽  
pp. 1153-1162 ◽  
Author(s):  
David Schrama ◽  
Sonja Hesbacher ◽  
Sabrina Angermeyer ◽  
Andreas Schlosser ◽  
Sebastian Haferkamp ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals RB1 is the crucial target of the Merkel cell polyomavirus Large T antigen in Merkel cell carcinoma cells

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32956-32968 ◽  
Author(s):  
Sonja Hesbacher ◽  
Lisa Pfitzer ◽  
Katharina Wiedorfer ◽  
Sabrina Angermeyer ◽  
Andreas Borst ◽  
...  
Keyword(s):  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen

scholarly journals Merkel Cell Polyomavirus Large T Antigen is Dispensable in G2 and M-Phase to Promote Proliferation of Merkel Cell Carcinoma Cells

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1162
Author(s):  
Roland Houben ◽  
Marlies Ebert ◽  
Sonja Hesbacher ◽  
Thibault Kervarrec ◽  
David Schrama
Keyword(s):  
Cell Cycle ◽  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Carcinoma Cells ◽  
Merkel Cell ◽  
Large T Antigen ◽  
M Phase ◽  
G2 Phase

Merkel cell carcinoma (MCC) is an aggressive skin cancer frequently caused by the Merkel cell polyomavirus (MCPyV), and proliferation of MCPyV-positive MCC tumor cells depends on the expression of a virus-encoded truncated Large T antigen (LT) oncoprotein. Here, we asked in which phases of the cell cycle LT activity is required for MCC cell proliferation. Hence, we generated fusion-proteins of MCPyV-LT and parts of geminin (GMMN) or chromatin licensing and DNA replication factor1 (CDT1). This allowed us to ectopically express an LT, which is degraded either in the G1 or G2 phase of the cell cycle, respectively, in MCC cells with inducible T antigen knockdown. We demonstrate that LT expressed only in G1 is capable of rescuing LT knockdown-induced growth suppression while LT expressed in S and G2/M phases fails to support proliferation of MCC cells. These results suggest that the crucial function of LT, which has been demonstrated to be inactivation of the cellular Retinoblastoma protein 1 (RB1) is only required to initiate S phase entry.

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