scholarly journals Usefulness of Immunocytochemistry for the Detection of the BRAFV600E Mutation in Circulating Tumor Cells from Metastatic Melanoma Patients

2013 ◽  
Vol 133 (5) ◽  
pp. 1378-1381 ◽  
Author(s):  
Véronique Hofman ◽  
Marius Ilie ◽  
Elodie Long-Mira ◽  
Damien Giacchero ◽  
Catherine Butori ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Brafv600e Mutation ◽  
Melanoma Patients

scholarly journals Prospective Molecular Profiling of Circulating Tumor Cells from Patients with Melanoma Receiving Combinatorial Immunotherapy

2019 ◽  
Vol 66 (1) ◽  
pp. 169-177 ◽  
Author(s):  
Selena Y Lin ◽  
Shu-Ching Chang ◽  
Stella Lam ◽  
Romela Irene Ramos ◽  
Kevin Tran ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Tumor Cells ◽  
Clinical Outcomes ◽  
Circulating Tumor Cells ◽  
Progressive Disease ◽  
Molecular Profiling ◽  
Braf V600e ◽  
Blood Samples ◽  
Mrna Profiling ◽  
Melanoma Patients

Abstract BACKGROUND Blood molecular profiling of circulating tumor cells (CTCs) can enable monitoring of patients with metastatic melanoma during checkpoint inhibitor immunotherapy (CII) and in combination with targeted therapies. We developed a microfluidics-based CTC platform to explore CTC profiling utility in CII-treated patients with melanoma using a melanoma messenger RNA (mRNA)/DNA biomarker panel. METHODS Blood samples (n = 213) were collected prospectively from 75 American Joint Committee on Cancer-staged III/IV melanoma patients during CII treatment and those enriched for CTCs. CTC profiling was performed using 5 known melanoma mRNA biomarkers and BRAF V600E DNA mutation. CTC biomarker status associations with clinical outcomes were assessed. RESULTS CTCs were detected in 88% of blood samples from patients with melanoma. CTC-derived biomarkers and clinical variables analyzed using classification and regression tree analysis revealed that a combination of lactate dehydrogenase, CTC-mRNA biomarkers, and tumor BRAF–mutation status was indicative of clinical outcomes for patients with stage IV melanoma (n = 52). The panel stratified low-risk and high-risk patients, whereby the latter had poor disease-free (P = 0.03) and overall survival (P = 0.02). Incorporation of a DNA biomarker with mRNA profiling increased overall CTC-detection capability by 57% compared to mRNA profiling only. RNA sequencing of isolated CTCs identified significant catenin beta 1 (CTNNB1) overexpression (P <0.01) compared to nondisease donor blood. CTC-CTNNB1 was associated with progressive disease/stable disease compared to complete-responder patient status (P = 0.02). Serial CTC profiling identified subclinical disease in patients who developed progressive disease during treatment/follow-up. CONCLUSIONS CTC-derived mRNA/DNA biomarkers have utility for monitoring CII, targeted, and combinatorial therapies in metastatic melanoma patients.

scholarly journals Circulating Tumor Cells in Melanoma Patients

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41052 ◽  
Author(s):  
Gary A. Clawson ◽  
Eric Kimchi ◽  
Susan D. Patrick ◽  
Ping Xin ◽  
Ramdane Harouaka ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Melanoma Patients

Circulating Tumor Cells in Stage IV Melanoma Patients

2018 ◽  
Vol 227 (1) ◽  
pp. 116-124 ◽  
Author(s):  
Carolyn S. Hall ◽  
Merrick Ross ◽  
Jessica B. Bowman Bauldry ◽  
Joshua Upshaw ◽  
Mandar G. Karhade ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Stage Iv ◽  
Melanoma Patients ◽  
Stage Iv Melanoma

scholarly journals Close proximity of immune and tumor cells underlies response to anti-PD-1 based therapies in metastatic melanoma patients

2019 ◽  
Vol 9 (1) ◽  
pp. 1659093 ◽  
Author(s):  
Tuba N. Gide ◽  
Ines P. Silva ◽  
Camelia Quek ◽  
Tasnia Ahmed ◽  
Alexander M. Menzies ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Tumor Cells ◽  
Close Proximity ◽  
Melanoma Patients

Evaluation of the liquid biopsy for the detection of BRAFV600E mutation in metastatic melanoma patients

2019 ◽  
Vol 26 (3) ◽  
pp. 271-279 ◽  
Author(s):  
Francesca Salvianti ◽  
Daniela Massi ◽  
Vincenzo De Giorgi ◽  
Alessia Gori ◽  
Mario Pazzagli ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Liquid Biopsy ◽  
Brafv600e Mutation ◽  
Melanoma Patients

Photoacoustic detection of circulating tumor cells in blood samples of stage III melanoma patients.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. 9555-9555
Author(s):  
John Andrew Viator ◽  
Benjamin Goldschmidt ◽  
Ahmad A. Tarhini ◽  
Cindy Sander ◽  
Martin Sanders
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Stage Iii ◽  
Photoacoustic Detection ◽  
Blood Samples ◽  
Melanoma Patients ◽  
Stage Iii Melanoma

Abstract 3159: BRAFV600E mutation analysis in circulating tumor cells

2011 ◽  
Author(s):  
Margaret Pace ◽  
Vladislava O. Melnikova ◽  
Kerry Brandl ◽  
Larry Kahn ◽  
Jacky Woo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Mutation Analysis ◽  
Brafv600e Mutation
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