scholarly journals Distinct Gene Expression Profiles of Viral- and Nonviral-Associated Merkel Cell Carcinoma Revealed by Transcriptome Analysis

2013 ◽  
Vol 133 (4) ◽  
pp. 936-945 ◽  
Author(s):  
Paul W. Harms ◽  
Rajiv M. Patel ◽  
Monique E. Verhaegen ◽  
Thomas J. Giordano ◽  
Kevin T. Nash ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Carcinoma ◽  
Merkel Cell Carcinoma ◽  
Transcriptome Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Merkel Cell ◽  
Distinct Gene

scholarly journals Merkel Cell Polyomavirus Downregulates N-myc Downstream-Regulated Gene 1, Leading to Cellular Proliferation and Migration

2019 ◽  
Vol 94 (3) ◽  
Author(s):  
Purnima Gupta ◽  
Naveed Shahzad ◽  
Alexis Harold ◽  
Masahiro Shuda ◽  
Assunta Venuti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Carcinoma ◽  
Cellular Proliferation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
T Antigen ◽  
Merkel Cell Polyomavirus ◽  
Early Gene ◽  
Merkel Cell ◽  
Gene Expressing

ABSTRACT Merkel cell polyomavirus (MCPyV) is the first human polyomavirus etiologically associated with Merkel cell carcinoma (MCC), a rare and aggressive form of skin cancer. Similar to other polyomaviruses, MCPyV encodes early T antigen genes, viral oncogenes required for MCC tumor growth. To identify the unique oncogenic properties of MCPyV, we analyzed the gene expression profiles in human spontaneously immortalized keratinocytes (NIKs) expressing the early genes from six distinct human polyomaviruses (PyVs), including MCPyV. A comparison of the gene expression profiles revealed 28 genes specifically deregulated by MCPyV. In particular, the MCPyV early gene downregulated the expression of the tumor suppressor gene N-myc downstream-regulated gene 1 (NDRG1) in MCPyV gene-expressing NIKs and hTERT-MCPyV gene-expressing human keratinocytes (HK) compared to their expression in the controls. In MCPyV-positive MCC cells, the expression of NDRG1 was downregulated by the MCPyV early gene, as T antigen knockdown rescued the level of NDRG1. In addition, NDRG1 overexpression in hTERT-MCPyV gene-expressing HK or MCC cells resulted in a decrease in the number of cells in S phase and cell proliferation inhibition. Moreover, a decrease in wound healing capacity in hTERT-MCPyV gene-expressing HK was observed. Further analysis revealed that NDRG1 exerts its biological effect in Merkel cell lines by regulating the expression of the cyclin-dependent kinase 2 (CDK2) and cyclin D1 proteins. Overall, NDRG1 plays an important role in MCPyV-induced cellular proliferation. IMPORTANCE Merkel cell carcinoma was first described in 1972 as a neuroendocrine tumor of skin, most cases of which were reported in 2008 to be caused by a PyV named Merkel cell polyomavirus (MCPyV), the first PyV linked to human cancer. Thereafter, numerous studies have been conducted to understand the etiology of this virus-induced carcinogenesis. However, it is still a new field, and much work is needed to understand the molecular pathogenesis of MCC. In the current work, we sought to identify the host genes specifically deregulated by MCPyV, as opposed to other PyVs, in order to better understand the relevance of the genes analyzed on the biological impact and progression of the disease. These findings open newer avenues for targeted drug therapies, thereby providing hope for the management of patients suffering from this highly aggressive cancer.

scholarly journals Transcriptome analysis of controlled and therapy-resistant childhood asthma reveals distinct gene expression profiles

2015 ◽  
Vol 136 (3) ◽  
pp. 638-648 ◽  
Author(s):  
Helena Persson ◽  
Andrew T. Kwon ◽  
Jordan A. Ramilowski ◽  
Gilad Silberberg ◽  
Cilla Söderhäll ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptome Analysis ◽  
Childhood Asthma ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Distinct Gene

scholarly journals Transcriptome analysis reveals distinct gene expression profiles in astrocytoma grades II-IV

2017 ◽  
Vol 161 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Nato Narsia ◽  
Pradeep Ramagiri ◽  
Jiri Ehrmann ◽  
Zdenek Kolar
Keyword(s):  
Gene Expression ◽  
Transcriptome Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Distinct Gene

scholarly journals Transcriptome analysis of gravitational effects on mouse skeletal muscles under microgravity and artificial 1 g onboard environment

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Risa Okada ◽  
Shin-ichiro Fujita ◽  
Riku Suzuki ◽  
Takuto Hayashi ◽  
Hirona Tsubouchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Muscle Mass ◽  
Muscle Atrophy ◽  
Transcriptome Analysis ◽  
Fiber Type ◽  
Expression Profiles ◽  
Space Station ◽  
Gene Expression Profiles

AbstractSpaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups in orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g; AG) and microgravity (μg; MG), to investigate whether artificial 1 g exposure prevents muscle atrophy at the molecular level. Our main findings indicated that AG onboard environment prevented changes under microgravity in soleus muscle not only in muscle mass and fiber type composition but also in the alteration of gene expression profiles. In particular, transcriptome analysis suggested that AG condition could prevent the alterations of some atrophy-related genes. We further screened novel candidate genes to reveal the muscle atrophy mechanism from these gene expression profiles. We suggest the potential role of Cacng1 in the atrophy of myotubes using in vitro and in vivo gene transductions. This critical project may accelerate the elucidation of muscle atrophy mechanisms.

Sign in / Sign up

Export Citation Format

Share Document