scholarly journals Application of an Indoleamine 2,3-Dioxygenase–Expressing Skin Substitute Improves Scar Formation in a Fibrotic Animal Model

2012 ◽  
Vol 132 (5) ◽  
pp. 1501-1505 ◽  
Author(s):  
Claudia Chavez-Munoz ◽  
Ryan Hartwell ◽  
Reza B. Jalili ◽  
Matthew Carr ◽  
Ruhangiz T. Kilani ◽  
...  
Keyword(s):  
Animal Model ◽  
Scar Formation ◽  
Skin Substitute ◽  
Indoleamine 2,3 Dioxygenase

Exploration of an Animal Model for Hypertrophic Scar Formation

1999 ◽  
Author(s):  
Erik S. Sojka ◽  
Ulysses Balis ◽  
Robert Sheridan ◽  
Alex Fowler
Keyword(s):  
Animal Model ◽  
Human Skin ◽  
Human Tissue ◽  
Hypertrophic Scar ◽  
Scar Formation ◽  
Mouse Tissue ◽  
Athymic Nude Mice ◽  
Human Dermis ◽  
Tissue Grafts ◽  
Positive Results

Abstract In this paper we investigate the possibility of creating an animal model for hypertrophic scar formation by grafting healthy split thickness human skin onto the backs of athymic nude mice and then burning the grafts. We were able to show that the human tissue grafts do remain stable over extended periods and prove that the human dermis does not get infiltrated by mouse tissue. By burning the grafts for ten seconds at 70°C we found that we could not reproduce results previously reported; but by changing the burn parameters we were able to produce regions of dermal disorder that may indicate the formation of hypertrophic scar. These results represent the first replication of positive results using this animal model since it was first proposed 10 years ago.

scholarly journals Local Expression of Indoleamine 2,3-Dioxygenase Protects Engraftment of Xenogeneic Skin Substitute

2006 ◽  
Vol 126 (1) ◽  
pp. 128-136 ◽  
Author(s):  
Yunyuan Li ◽  
Edward E. Tredget ◽  
Abdi Ghaffari ◽  
Xiaoyue Lin ◽  
Ruhangiz T. Kilani ◽  
...  
Keyword(s):  
Skin Substitute ◽  
Indoleamine 2,3 Dioxygenase ◽  
Local Expression

scholarly journals Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2584
Author(s):  
Won Seok Choi ◽  
Joo Hyun Kim ◽  
Chi Bum Ahn ◽  
Ji Hyun Lee ◽  
Yu Jin Kim ◽  
...  
Keyword(s):  
3D Printing ◽  
Scar Formation ◽  
Skin Substitute ◽  
Reconstruction Method ◽  
Skin Substitutes ◽  
Nanofiber Scaffold ◽  
Tissue Reconstruction ◽  
Multiple Cells ◽  
Cell Adhesion And Proliferation ◽  
New Skin

Large-sized or deep skin wounds require skin substitutes for proper healing without scar formation. Therefore, multi-layered skin substitutes that mimic the genuine skin anatomy of multiple layers have attracted attention as suitable skin substitutes. In this study, a novel skin substitute was developed by combining the multi-layer skin tissue reconstruction method with the combination of a human-derived keratinic extract-loaded nano- and micro-fiber using electrospinning and a support structure using 3D printing. A polycaprolactone PCL/keratin electrospun scaffold showed better cell adhesion and proliferation than the keratin-free PCL scaffold, and keratinocytes and fibroblasts showed better survival, adhesion, and proliferation in the PCL/keratin electrospun nanofiber scaffold and microfiber scaffold, respectively. In a co-culture of keratinocytes and fibroblasts using a multi-layered scaffold, the two cells formed the epidermis and dermal layer on the PCL/keratin scaffold without territorial invasion. In the animal study, the PCL/keratin scaffold caused a faster regeneration of new skin without scar formation compared to the PCL scaffold. Our study showed that PCL/keratin scaffolds co-cultured with keratinocytes and fibroblasts promoted the regeneration of the epidermal and dermal layers in deep skin defects. Such finding suggests a new possibility for artificial skin production using multiple cells.

Local Expression of Indoleamine 2,3-dioxygenase (IDO) Protects Engraftment of Xenogeneic Skin Substitute in Rat

2006 ◽  
Vol 27 (Supplement) ◽  
pp. S113
Author(s):  
Y Li ◽  
E E. Tredget ◽  
A Ghaffari ◽  
X Lin ◽  
R T. Kilani ◽  
...  
Keyword(s):  
Skin Substitute ◽  
Indoleamine 2,3 Dioxygenase ◽  
Local Expression

Immune Regulatory Function of Dendritic Cells Expressing Indoleamine 2,3-Dioxygenase in Orally Tolerance to Type II Collagen-induced Animal Model

2005 ◽  
Vol 5 (4) ◽  
pp. 221
Author(s):  
Min Jung Park ◽  
So Youn Min ◽  
Kyoung Su Park ◽  
Mi La Cho ◽  
Young-Gyu Cho ◽  
...  
Keyword(s):  
Animal Model ◽  
Dendritic Cells ◽  
Type Ii Collagen ◽  
Regulatory Function ◽  
Type Ii ◽  
Indoleamine 2,3 Dioxygenase

Local expression of indoleamine 2,3-dioxygenase suppresses T-cell-mediated rejection of an engineered bilayer skin substitute

2010 ◽  
Vol 18 (6) ◽  
pp. 614-623 ◽  
Author(s):  
Farshad Forouzandeh ◽  
Reza B. Jalili ◽  
Ryan V. Hartwell ◽  
Sarah E. Allan ◽  
Steven Boyce ◽  
...  
Keyword(s):  
T Cell ◽  
Skin Substitute ◽  
Indoleamine 2,3 Dioxygenase ◽  
Local Expression
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