scholarly journals Gene Expression in Peripheral Blood Differs after Cardioembolic Compared with Large-Vessel Atherosclerotic Stroke: Biomarkers for the Etiology of Ischemic Stroke

2008 ◽  
Vol 28 (7) ◽  
pp. 1320-1328 ◽  
Author(s):  
Huichun Xu ◽  
Yang Tang ◽  
Da-Zhi Liu ◽  
Ruiqiong Ran ◽  
Bradley P Ander ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ischemic Stroke ◽  
Peripheral Blood ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Large Vessel ◽  
Cardioembolic Stroke ◽  
Unknown Etiology ◽  
Stroke Patients ◽  
Clinical Criteria

There are no biomarkers that differentiate cardioembolic from large-vessel atherosclerotic stroke, although the treatments differ for each and ~30% of strokes and transient ischemic attacks have undetermined etiologies using current clinical criteria. We aimed to define gene expression profiles in blood that differentiate cardioembolic from large-vessel atherosclerotic stroke. Peripheral blood samples were obtained from healthy controls and acute ischemic stroke patients (< 3, 5, and 24 h). RNA was purified, labeled, and applied to Affymetrix Human U133 Plus 2.0 Arrays. Expression profiles in the blood of cardioembolic stroke patients are distinctive from those of large-vessel atherosclerotic stroke patients. Seventy-seven genes differ at least 1.5-fold between them, and a minimum number of 23 genes differentiate the two types of stroke with at least 95.2% specificity and 95.2% sensitivity for each. Genes regulated in large-vessel atherosclerotic stroke are expressed in platelets and monocytes and modulate hemostasis. Genes regulated in cardioembolic stroke are expressed in neutrophils and modulate immune responses to infectious stimuli. This new method can be used to predict whether a stroke of unknown etiology was because of cardioembolism or large-vessel atherosclerosis that would lead to different therapy. These results have wide ranging implications for similar disorders.

scholarly journals Brief Focal Cerebral Ischemia That Simulates Transient Ischemic Attacks in Humans Regulates Gene Expression in Rat Peripheral Blood

2009 ◽  
Vol 30 (1) ◽  
pp. 110-118 ◽  
Author(s):  
Xinhua Zhan ◽  
Bradley P Ander ◽  
Glen Jickling ◽  
Renée Turner ◽  
Boryana Stamova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Ischemic Stroke ◽  
Cerebral Ischemia ◽  
Peripheral Blood ◽  
Focal Cerebral Ischemia ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Focal Ischemia ◽  
Transient Ischemic Attacks

Blood gene expression profiles of very brief (5 and 10 mins) focal ischemia that simulates transient ischemic attacks in humans were compared with ischemic stroke (120 mins focal ischemia), sham, and naïve controls. The number of significantly regulated genes after 5 and 10 mins of cerebral ischemia was 39 and 160, respectively (fold change ⩾∣1.5∣ and P<0.05). There were 103 genes common to brief focal ischemia and ischemic stroke. Ingenuity pathway analysis showed that genes regulated in the 5 mins group were mainly involved in small molecule biochemistry. Genes regulated in the 10 mins group were involved in cell death, development, growth, and proliferation. Such genes were also regulated in the ischemic stroke group. Genes common to ischemia were involved in the inflammatory response, immune response, and cell death—indicating that these pathways are a feature of focal ischemia, regardless of the duration. These results provide evidence that brief focal ischemia differentially regulates gene expression in the peripheral blood in a manner that could distinguish brief focal ischemia from ischemic stroke and controls in rats. We postulate that this will also occur in humans.

Comparison of gene expression profiles of T cells in porcine colostrum and peripheral blood

2016 ◽  
Vol 77 (9) ◽  
pp. 961-968 ◽  
Author(s):  
Shohei Ogawa ◽  
Mie Okutani ◽  
Takamitsu Tsukahara ◽  
Nobuo Nakanishi ◽  
Yoshihiro Kato ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Peripheral Blood ◽  
Expression Profiles ◽  
Gene Expression Profiles

scholarly journals Differences in metabolic biomarkers in the blood and gene expression profiles of peripheral blood mononuclear cells among normal weight, mildly obese and moderately obese subjects

2016 ◽  
Vol 116 (6) ◽  
pp. 1022-1032 ◽  
Author(s):  
Un Ju Jung ◽  
Yu Ri Seo ◽  
Ri Ryu ◽  
Myung-Sook Choi
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Peripheral Blood ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Hdl Cholesterol ◽  
Normal Weight ◽  
Peripheral Blood Mononuclear ◽  
Obese Subjects ◽  
Metabolic Biomarkers

AbstractWe compared metabolic biomarkers in the blood and peripheral blood mononuclear cell (PBMC) gene expression profiles among normal weight (BMI, 18·5–23 kg/m2), mildly obese (BMI, 25–27·5 kg/m2) and moderately obese Korean adult men (BMI, 27·5–30 kg/m2). High leptin, lipids (except LDL- and HDL-cholesterol) and apoB levels and low adiponectin and HDL-cholesterol levels were present in the plasma of both mildly and moderately obese subjects. Circulating levels of inflammatory cytokines and markers of insulin resistance, oxidative stress and liver damage were altered in moderately obese subjects but not in mildly obese subjects. PBMC transcriptome data showed enrichment of pathways involved in energy metabolism, insulin resistance, bone metabolism, cancer, inflammation and fibrosis in both mildly and moderately obese subjects. Signalling pathways involved in oxidative phosphorylation, TAG synthesis, carbohydrate metabolism and insulin production; mammalian target of rapamycin, forkhead box O, ras-proximate-1, RAS and transforming growth factor-β signalling; as well as extracellular matrix–receptor interaction were enriched only in moderately obese subjects, indicating that changes in PBMC gene expression profiles, according to metabolic disturbances, were associated with the development and/or aggravation of obesity. In particular, fourteen and fifteen genes differentially expressed only in mildly obese subjects and in both mildly and moderately obese subjects, respectively, could be used as early or stable biomarkers for diagnosing and treating obesity-associated metabolic disturbance. We characterised BMI-associated metabolic and molecular biomarkers in the blood and provided clues about potential blood-based targets for preventing or treating obesity-related complications.

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