Changes in Brain Monoaminergic Neurotransmitter Concentrations in Rat after Intracerebroventricular Injection of Streptozotocin

1992 ◽  
Vol 12 (1) ◽  
pp. 103-109 ◽  
Author(s):  
Andreas Ding ◽  
Roger Nitsch ◽  
Siegfried Hoyer
Keyword(s):  
Frontal Cortex ◽  
Electrochemical Detection ◽  
Entorhinal Cortex ◽  
Turnover Rate ◽  
Brain Metabolism ◽  
Intracerebroventricular Injection ◽  
Brain Areas ◽  
Tissue Concentrations ◽  
Hydroxyindoleacetic Acid ◽  
Monoaminergic Neurotransmitter

The tissue concentrations of the monoaminergic neurotransmitters noradrenaline (NA), dopamine, and serotonin (5-HT) and of their major metabolites were measured by HPLC and electrochemical detection in several rat brain areas after intracerebroventricular injection of streptozotocin (STZ). NA levels were found to be decreased in the frontal cortex by 14%, in the entorhinal cortex by 18%, and in the striatum by 38%. In the entorhinal cortex, 5-HT levels were decreased by 19% and the 5-HT turnover rate, measured as the 5-hydroxyindoleacetic acid/5-HT ratio, was found to be increased by 48%. These results may be indicative of a distinct susceptibility of some neurotransmitters in certain brain areas after a more general impairment of brain metabolism by means of intracerebroventricular application of the diabetogenic compound STZ.

Serotonin and 5-hydroxyindoleacetic acid concentrations in individual hypothalamic nuclei and other brain areas of rat

1984 ◽  
Vol 40 (11) ◽  
pp. 1288-1290 ◽  
Author(s):  
K. Oomagari ◽  
H. Uchimura ◽  
T. Matsumoto ◽  
H. Yokoo ◽  
M. Hirano ◽  
...  
Keyword(s):  
Brain Areas ◽  
Hypothalamic Nuclei ◽  
Hydroxyindoleacetic Acid

Catabolism of intracerebroventricularly injected 5-hydroxytryptamine in mouse: effect of coinjection of tryptamine and several pretreatments

1993 ◽  
Vol 71 (3-4) ◽  
pp. 201-204 ◽  
Author(s):  
B. Duff Sloley ◽  
Shuzo Orikasa ◽  
Alan A. Boulton
Keyword(s):  
Monoamine Oxidase ◽  
Receptor Antagonist ◽  
Mouse Brain ◽  
Monoamine Oxidase Inhibitor ◽  
Oxidase Inhibitor ◽  
Nerve Terminals ◽  
Intracerebroventricular Injection ◽  
Hydroxyindoleacetic Acid

The catabolism of intracerebroventricularly injected 5-hydroxytryptamine in mouse brain was investigated. Pretreatment of animals with the 5-hydroxytryptamine type 1 receptor antagonist metergoline, the 5-hydroxytryptamine type 2 receptor antagonist ketanserin, the 5-hydroxytryptamine reuptake inhibitor fluoxetine, or the selective 5-hydroxytryptamine neurotoxin 5,7-dihydroxytryptamine failed to alter the rate of catabolism of intracerebroventricularly administered 5-hydroxytryptamine. The monoamine oxidase inhibitor tranylcypromine effectively blocked degradation of injected 5-hydroxytryptamine and accumulation of 5-hydroxyindoleacetic acid. Coinjection of tryptamine with 5-hydroxytryptamine reduced the rate of conversion of 5-hydroxytryptamine to 5-hydroxyindoleacetic acid. These results indicate that intracerebroventricularly administered 5-hydroxytryptamine is removed by a monoamine oxidase dependent system. This catabolism is not affected by inhibition of presynaptic uptake, 5-hydroxytryptamine receptor type 1 or type 2 blockade, or destruction of serotonergic nerve terminals. The coadministration of tryptamine may prolong the residence period of 5-hydroxytryptamine through competition for monoamine oxidase.Key words: 5-hydroxytryptamine, tryptamine, monoamine oxidase, intracerebroventricular injection, catabolism.

Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection.

1984 ◽  
Vol 30 (1) ◽  
pp. 131-135 ◽  
Author(s):  
P C Tagari ◽  
D J Boullin ◽  
C L Davies
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Electrochemical Detection ◽  
High Performance ◽  
Carcinoid Syndrome ◽  
Quantitative Detection ◽  
Chromatographic Purification ◽  
Hydroxyindoleacetic Acid

Abstract This simplified determination of serotonin in human plasma involves "high-performance" liquid chromatography with electrochemical detection. Deproteinated samples of platelet-poor plasma are injected directly into the chromatograph without preliminary chromatographic purification. The limit of quantitative detection of serotonin in plasma is 5 nmol/L (equivalent to 250 fmol injected). Intra- and inter-assay CVs ranged from 5.6% to 7.7% and 3.5% to 14.9%, respectively, for concentrations of serotonin from 0.02 to 10.0 mumol/L. Results agreed well with those by spectrofluorometry, both for plasma standards (r = 0.985, range 100 nmol/L to 100 mumol/L) and for pathological samples obtained from four carcinoid syndrome patients (r = 0.964). The method is also applicable to the concurrent measurement of 5-hydroxytryptophan and 5-hydroxyindoleacetic acid.

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