scholarly journals Molecular beacons to identify gifted microbes for genome mining

2017 ◽  
Vol 70 (5) ◽  
pp. 639-646 ◽  
Author(s):  
Richard H Baltz
Keyword(s):  
Genome Mining ◽  
Molecular Beacons ◽  
Gifted Microbes

Photo-tethered molecular beacons for superior light-induction

2021 ◽  
Author(s):  
Robin Klimek ◽  
Mantian Wang ◽  
Vivien R. McKenney ◽  
Erin M. Schuman ◽  
Alexander Heckel
Keyword(s):  
Molecular Beacons ◽  
Light Induction ◽  
In Cells

Photolabile circularization of molecular beacons via backbone phosphates leads to superior probes to study spatiotemporal aspects of RNA in cells.

Discovery of fatty acid synthase inhibitors and their biosynthetic pathways by a novel target-directed genome mining strategy

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
J Li ◽  
X Tang ◽  
JJ Zhang ◽  
EC O'Neill ◽  
SM Mantovani ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Genome Mining ◽  
Biosynthetic Pathways ◽  
Novel Target

Genome mining and characterisation of multiple bioactive compounds from a Burkholderia gladioli isolate collection

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
C Jones ◽  
MJ Bull ◽  
M Jenner ◽  
L Song ◽  
Y Dashti ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Genome Mining ◽  
Burkholderia Gladioli

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

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