scholarly journals New natural products isolated from Metarhizium robertsii ARSEF 23 by chemical screening and identification of the gene cluster through engineered biosynthesis in Aspergillus nidulans A1145

2016 ◽  
Vol 69 (7) ◽  
pp. 561-566 ◽  
Author(s):  
Hiroki Kato ◽  
Yuta Tsunematsu ◽  
Tsuyoshi Yamamoto ◽  
Takuya Namiki ◽  
Shinji Kishimoto ◽  
...  
Keyword(s):  
Natural Products ◽  
Aspergillus Nidulans ◽  
Gene Cluster ◽  
Metarhizium Robertsii ◽  
Chemical Screening ◽  
Screening And Identification

Unzipping Natural Products: Improved Natural Product Structure Predictions by Ensemble Modeling and Fingerprint Matching

2018 ◽  
Author(s):  
William A. Shirley ◽  
Brian P. Kelley ◽  
Yohann Potier ◽  
John H. Koschwanez ◽  
Robert Bruccoleri ◽  
...  
Keyword(s):  
Natural Products ◽  
Open Source ◽  
Natural Product ◽  
Gene Cluster ◽  
Public Domain ◽  
Product Structure ◽  
Fingerprint Matching ◽  
Ensemble Modeling ◽  
Chemical Structures ◽  
Source Gene

This pre-print explores ensemble modeling of natural product targets to match chemical structures to precursors found in large open-source gene cluster repository antiSMASH. Commentary on method, effectiveness, and limitations are enclosed. All structures are public domain molecules and have been reviewed for release.

Characterization and analysis of an industrial strain of Streptomyces bingchenggensis by genome sequencing and gene microarray

Genome ◽  
2013 ◽  
Vol 56 (11) ◽  
pp. 677-689 ◽  
Author(s):  
Xiang-Jing Wang ◽  
Bo Zhang ◽  
Yi-Jun Yan ◽  
Jing An ◽  
Ji Zhang ◽  
...  
Keyword(s):  
Natural Products ◽  
Gene Cluster ◽  
Crop Protection ◽  
Gc Content ◽  
Gene Clusters ◽  
High Expression ◽  
Expression Level ◽  
Transcriptional Analysis ◽  
Streptomyces Bingchenggensis ◽  
High Expression Level

Streptomyces bingchenggensis is a soil bacterium that produces milbemycins, a family of macrolide antibiotics that are commercially important in crop protection and veterinary medicine. In addition, S. bingchenggensis produces many other natural products including the polyether nanchangmycin and novel cyclic pentapeptides. To identify the gene clusters involved in the biosynthesis of these compounds, and better clarify the biochemical pathways of these gene clusters, the whole genome of S. bingchenggensis was sequenced, and the transcriptome profile was subsequently investigated by microarray. In comparison with other sequenced genomes in Streptomyces, S. bingchenggensis has the largest linear chromosome consisting of 11 936 683 base pairs (bp), with an average GC content of 70.8%. The 10 023 predicted protein-coding sequences include at least 47 gene clusters correlated with the biosynthesis of known or predicted secondary metabolites. Transcriptional analysis demonstrated an extremely high expression level of the milbemycin gene cluster during the entire growth period and a moderately high expression level of the nanchangmycin gene cluster during the initial hours that subsequently decreased. However, other gene clusters appear to be silent. The genome-wide analysis of the secondary metabolite gene clusters in S. bingchenggensis, coupled with transcriptional analysis, will facilitate the rational development of high milbemycins-producing strains as well as the discovery of new natural products.

scholarly journals mSphere of Influence: Synthetic Biology of Natural Product Biosynthesis

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Mark C. Walker
Keyword(s):  
Natural Products ◽  
Synthetic Biology ◽  
Natural Product ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Natural Product Biosynthesis ◽  
Antibiotic Compounds ◽  
Approaches To Study

ABSTRACT Mark Walker studies the biosynthesis and engineering of bacterial natural products with the long-term goal of identifying new antibiotic compounds. In this mSphere of Influence, he reflects on how “Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A” by K. Yamanaka, K. A. Reynolds, R. D. Kersten, K. S. Ryan, et al. (Proc Natl Acad Sci USA 111:1957–1962, 2014, https://doi.org/10.1073/pnas.1319584111) impacted his thinking on using synthetic biology approaches to study natural product biosynthesis.

A Chemical Screening Strategy for the Dereplication and Prioritization of HIV-Inhibitory Aqueous Natural Products Extracts

1993 ◽  
Vol 56 (7) ◽  
pp. 1123-1129 ◽  
Author(s):  
John H. Cardellina ◽  
Murray H. G. Munro ◽  
Richard W. Fuller ◽  
Kirk P. Manfredi ◽  
Tawnya C. McKee ◽  
...  
Keyword(s):  
Natural Products ◽  
Screening Strategy ◽  
Chemical Screening

Deletion of a Histone Acetyltransferase Leads to the Pleiotropic Activation of Natural Products in Metarhizium robertsii

2017 ◽  
Vol 19 (7) ◽  
pp. 1686-1689 ◽  
Author(s):  
Aili Fan ◽  
Wubin Mi ◽  
Zhiguo Liu ◽  
Guohong Zeng ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Natural Products ◽  
Histone Acetyltransferase ◽  
Metarhizium Robertsii
Sign in / Sign up

Export Citation Format

Share Document