scholarly journals Identification of the biosynthetic gene cluster of A-500359s in Streptomyces griseus SANK60196

2009 ◽  
Vol 62 (6) ◽  
pp. 325-332 ◽  
Author(s):  
Masanori Funabashi ◽  
Koichi Nonaka ◽  
Chieko Yada ◽  
Masahiko Hosobuchi ◽  
Nobuhisa Masuda ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Streptomyces Griseus ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Gene Cluster Involved in the Biosynthesis of Griseobactin, a Catechol-Peptide Siderophore of Streptomyces sp. ATCC 700974

2009 ◽  
Vol 192 (2) ◽  
pp. 426-435 ◽  
Author(s):  
Silke I. Patzer ◽  
Volkmar Braun
Keyword(s):  
Gene Cluster ◽  
Linear Form ◽  
Streptomyces Griseus ◽  
Biosynthetic Gene Cluster ◽  
Peptide Structure ◽  
Biosynthetic Gene ◽  
Biosynthesis Gene Cluster ◽  
Streptomyces Sp ◽  
Peptide Synthetase ◽  
Biosynthesis Gene

ABSTRACT The main siderophores produced by streptomycetes are desferrioxamines. Here we show that Streptomyces sp. ATCC 700974 and several Streptomyces griseus strains, in addition, synthesize a hitherto unknown siderophore with a catechol-peptide structure, named griseobactin. The production is repressed by iron. We sequenced a 26-kb DNA region comprising a siderophore biosynthetic gene cluster encoding proteins similar to DhbABCEFG, which are involved in the biosynthesis of 2,3-dihydroxybenzoate (DHBA) and in the incorporation of DHBA into siderophores via a nonribosomal peptide synthetase. Adjacent to the biosynthesis genes are genes that encode proteins for the secretion, uptake, and degradation of siderophores. To correlate the gene cluster with griseobactin synthesis, the dhb genes in ATCC 700974 were disrupted. The resulting mutants no longer synthesized DHBA and griseobactin; production of both was restored by complementation with the dhb genes. Heterologous expression of the dhb genes or of the entire griseobactin biosynthesis gene cluster in the catechol-negative strain Streptomyces lividans TK23 resulted in the synthesis and secretion of DHBA or griseobactin, respectively, suggesting that these genes are sufficient for DHBA and griseobactin biosynthesis. Griseobactin was purified and characterized; its structure is consistent with a cyclic and, to a lesser extent, linear form of the trimeric ester of 2,3-dihydroxybenzoyl-arginyl-threonine complexed with aluminum under iron-limiting conditions. This is the first report identifying the gene cluster for the biosynthesis of DHBA and a catechol siderophore in Streptomyces.

Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus

1991 ◽  
Vol 229 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Dusica Vujaklija ◽  
Kenji Ueda ◽  
Soon-Kwang Hong ◽  
Teruhiko Beppu ◽  
Sueharu Horinouchi
Keyword(s):  
Gene Cluster ◽  
Streptomyces Griseus ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Identification and analysis of the biosynthetic gene cluster for the indolizidine alkaloid iminimycin in Streptomyces griseus

ChemBioChem ◽  
2021 ◽  
Author(s):  
Yohei Katsuyama ◽  
Hayama Tsutsumi ◽  
Takeaki Tezuka ◽  
Rei Miyano ◽  
Yuki Inahashi ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Streptomyces Griseus ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97

2019 ◽  
Vol 83 (11) ◽  
pp. 2082-2089 ◽  
Author(s):  
Jian Mo ◽  
Jiang Ye ◽  
Haozhe Chen ◽  
Bingbing Hou ◽  
Haizhen Wu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Streptomyces Griseus ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Anacyclamide D8P, a prenylated cyanobactin from a Sphaerospermopsis sp. cyanobacterium

2017 ◽  
Author(s):  
Joana Martins ◽  
Niina Leikoski ◽  
Matti Wahlsten ◽  
Joana Azevedo ◽  
Jorge Antunes ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Cyclic Peptides ◽  
Biosynthetic Gene Cluster ◽  
The Novel ◽  
Tyrosine Residue ◽  
Biosynthetic Gene ◽  
Post Translational Modification ◽  
Biological Assays ◽  
Mass Spectrometric Data ◽  
Spectrometric Data

Cyanobactins are a family of linear and cyclic peptides produced through the post-translational modification of short precursor peptides. Anacyclamides are macrocyclic cyanobactins with a highly diverse sequence that are common in the genus <i>Anabaena</i>. A mass spectrometry-based screening of potential cyanobactin producers led to the discovery of a new prenylated member of this family of compounds, anacyclamide D8P (<b>1</b>), from <i>Sphaerospermopsis</i> sp. LEGE 00249. The anacyclamide biosynthetic gene cluster (<i>acy</i>) encoding the novel macrocyclic prenylated cyanobactin, was sequenced. Heterologous expression of the acy gene cluster in <i>Escherichia</i> <i>coli</i> established the connection between genomic and mass spectrometric data. Unambiguous establishment of the type and site of prenylation required the full structural elucidation of <b>1</b> using Nuclear Magnetic Resonance (NMR), which demonstrated that a forward prenylation occurred on the tyrosine residue. Compound <b>1</b> was tested in pharmacologically or ecologically relevant biological assays and revealed moderate antimicrobial activity towards the fouling bacterium <i>Halomonas aquamarina</i> CECT 5000.<br>

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