The Mode of Action of 2-(Thiazol-2-ylthio)-1β-methylcarbapenems against Pseudomonas aeruginosa: The Impact of Outer Membrane Permeability and the Contribution of MexAB-OprM Efflux System

2007 ◽  
Vol 60 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Ken Eguchi ◽  
Yutaka Ueda ◽  
Katsunori Kanazawa ◽  
Makoto Sunagawa ◽  
Naomasa Gotoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Permeability ◽  
Outer Membrane ◽  
Mode Of Action ◽  
Efflux System ◽  
Outer Membrane Permeability ◽  
The Impact

scholarly journals Low-frequency ultrasound increases outer membrane permeability of Pseudomonas aeruginosa

2006 ◽  
Vol 52 (5) ◽  
pp. 295-301 ◽  
Author(s):  
Christopher M. Runyan ◽  
John C. Carmen ◽  
Benjamin L. Beckstead ◽  
Jared L. Nelson ◽  
Richard A. Robison ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Permeability ◽  
Outer Membrane ◽  
Low Frequency ◽  
Low Frequency Ultrasound ◽  
Outer Membrane Permeability ◽  
Frequency Ultrasound

Estimation of outer membrane permeability of carbapenem antibiotics to Pseudomonas aeruginosa

1999 ◽  
Vol 5 (3) ◽  
pp. 168-170 ◽  
Author(s):  
Shizuko Iyobe ◽  
Matsuhisa Inoue ◽  
Masato Watanabe ◽  
Susumu Mitsuhashi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Permeability ◽  
Outer Membrane ◽  
Outer Membrane Permeability

scholarly journals Investigation of the Antibacterial Activity and Efflux Pump Inhibitory Effect of Cycas thouarsii R.Br. Extract against Klebsiella pneumoniae Clinical Isolates

2021 ◽  
Vol 14 (8) ◽  
pp. 756
Author(s):  
Walaa A. Negm ◽  
Mona El-Aasr ◽  
Amal Abo Kamer ◽  
Engy Elekhnawy
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Membrane Permeability ◽  
Outer Membrane ◽  
Membrane Integrity ◽  
Membrane Depolarization ◽  
Clinical Isolates ◽  
Resistant Bacteria ◽  
Outer Membrane Permeability ◽  
The Impact

The vast spread of multidrug-resistant bacteria has encouraged researchers to explore new antimicrobial compounds. This study aimed to investigate the phytochemistry and antibacterial activity of Cycas thouarsii R.Br. leaves extract against Klebsiella pneumoniae clinical isolates. The minimum inhibitory concentration (MIC) values of C. thouarsii extract ranged from 4 to 32 µg/mL. The impact of the treatment of the isolates with sub-inhibitory concentrations of C. thouarsii extract was investigated on the bacterial growth, membrane integrity, inner and outer membrane permeability, membrane depolarization, and bacterial morphology using a scanning electron microscope (SEM) and on the efflux activity using qRT-PCR. Interestingly, most K. pneumoniae isolates treated with C. thouarsii extract showed growth inhibition—a decrease in membrane integrity. In addition, we observed various morphological changes, a significant increase in inner and outer membrane permeability, a non-significant change in membrane depolarization, and a decrease in efflux activity after treatment. The phytochemical investigation of C. thouarsii extract revealed the isolation of one new biflavonoid, 5,7,7”,4”’-tetra-O-methyl-hinokiflavone (3), and five known compounds, stigmasterol (1), naringenin (2), 2,3-dihydrobilobetin (4), 4’,4’’’-O-dimethyl amentoflavone (5), and hinokiflavone (6), for the first time. Moreover, the pure compounds’ MICs’ ranged from 0.25 to 2 µg/mL. Thus, C. thouarsii could be a potential source for new antimicrobials.

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