scholarly journals Diversity of environmental single-stranded DNA phages revealed by PCR amplification of the partial major capsid protein

2014 ◽  
Vol 8 (10) ◽  
pp. 2093-2103 ◽  
Author(s):  
Max Hopkins ◽  
Shweta Kailasan ◽  
Allison Cohen ◽  
Simon Roux ◽  
Kimberly Pause Tucker ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
Pcr Amplification ◽  
Single Stranded Dna

scholarly journals ICTV Virus Taxonomy Profile: Finnlakeviridae

2020 ◽  
Vol 101 (9) ◽  
pp. 894-895
Author(s):  
Sari Mäntynen ◽  
Elina Laanto ◽  
Lotta-Riina Sundberg ◽  
Minna M. Poranen ◽  
Hanna M. Oksanen ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
Sequence Similarity ◽  
International Committee ◽  
Host Bacterium ◽  
Protein Fold ◽  
Single Stranded Dna ◽  
Double Stranded Dna ◽  
Internal Membrane ◽  
The Family

Finnlakeviridae is a family of icosahedral, internal membrane-containing bacterial viruses with circular, single-stranded DNA genomes. The family includes the genus, Finnlakevirus, with the species, Flavobacterium virus FLiP. Flavobacterium phage FLiP was isolated with its Gram-negative host bacterium from a boreal freshwater habitat in Central Finland in 2010. It is the first described single-stranded DNA virus with an internal membrane and shares minimal sequence similarity with other known viruses. The virion organization (pseudo T=21 dextro) and major capsid protein fold (double-β-barrel) resemble those of Pseudoalteromonas phage PM2 (family Corticoviridae), which has a double-stranded DNA genome. A similar major capsid protein fold is also found in other double-stranded DNA viruses in the kingdom Bamfordvirae. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) report on the family Finnlakeviridae, which is available at ictv.global/report/finnlakeviridae.

scholarly journals Identification of a nonconventional motif necessary for the nuclear import of the human parvovirus B19 major capsid protein (VP2)

Virology ◽  
2003 ◽  
Vol 306 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Sylvie Pillet ◽  
Zeinab Annan ◽  
Serge Fichelson ◽  
F.rédéric Morinet
Keyword(s):  
Capsid Protein ◽  
Nuclear Import ◽  
Major Capsid Protein ◽  
Parvovirus B19 ◽  
Human Parvovirus B19

scholarly journals Polinton-like viruses are abundant in aquatic ecosystems

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Christopher M. Bellas ◽  
Ruben Sommaruga
Keyword(s):  
Capsid Protein ◽  
Major Capsid Protein ◽  
Virus Detection ◽  
Virus Genome ◽  
Alpine Lake ◽  
Virus Particles ◽  
Wide Range ◽  
Extracellular Form ◽  
Eukaryotic Organisms ◽  
Dsdna Viruses

Abstract Background Polintons are large mobile genetic elements found in the genomes of eukaryotic organisms that are considered the ancient ancestors of most eukaryotic dsDNA viruses. Originally considered as transposons, they have been found to encode virus capsid genes, suggesting they may actually be integrated viruses; however, an extracellular form has yet to be detected. Recently, circa 25 Polinton-like viruses have been discovered in environmental metagenomes and algal genomes, which shared distantly related genes to both Polintons and virophages (Lavidaviridae). These entities could be the first members of a major class of ancient eukaryotic viruses; however, owing to the lack of available genomes for analysis, information on their global diversity, evolutionary relationships, eukaryotic hosts, and status as free virus particles is limited. Results Here, we analysed the metaviromes of an alpine lake to show that Polinton-like virus genome sequences are abundant in the water column. We identify major capsid protein genes belonging to 82 new Polinton-like viruses and use these to interrogate publicly available metagenomic datasets, identifying 543 genomes and a further 16 integrated into eukaryotic genomes. Using an analysis of shared gene content and major capsid protein phylogeny, we define large groups of Polinton-like viruses and link them to diverse eukaryotic hosts, including a new group of viruses, which possess all the core genes of virophages and infect oomycetes and Chrysophyceae. Conclusions Our study increased the number of known Polinton-like viruses by 25-fold, identifying five major new groups of eukaryotic viruses, which until now have been hidden in metagenomic datasets. The large enrichment (> 100-fold) of Polinton-like virus sequences in the virus-sized fraction of this alpine lake and the fact that their viral major capsid proteins are found in eukaryotic host transcriptomes support the hypothesis that Polintons in unicellular eukaryotes are viruses. In summary, our data reveals a diverse assemblage of globally distributed viruses, associated with a wide range of unicellular eukaryotic hosts. We anticipate that the methods we have developed for Polinton-like virus detection and the database of over 20,000 genes we present will allow for continued discovery and analysis of these new viral groups.

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