scholarly journals High-throughput isotopic analysis of RNA microarrays to quantify microbial resource use

2011 ◽  
Vol 6 (6) ◽  
pp. 1210-1221 ◽  
Author(s):  
Xavier Mayali ◽  
Peter K Weber ◽  
Eoin L Brodie ◽  
Shalini Mabery ◽  
Paul D Hoeprich ◽  
...  
Keyword(s):  
High Throughput ◽  
Resource Use ◽  
Isotopic Analysis ◽  
Rna Microarrays

scholarly journals Non-invasive approaches for phenotyping of enhanced performance traits in bean

2011 ◽  
Vol 38 (12) ◽  
pp. 968 ◽  
Author(s):  
Uwe Rascher ◽  
Stephan Blossfeld ◽  
Fabio Fiorani ◽  
Siegfried Jahnke ◽  
Marcus Jansen ◽  
...  
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Resource Use ◽  
High Throughput Screening ◽  
Resource Competition ◽  
Plant Phenotyping ◽  
Plant Structure ◽  
Resource Use Efficiency ◽  
Use Efficiency ◽  
Structural Traits

Plant phenotyping is an emerging discipline in plant biology. Quantitative measurements of functional and structural traits help to better understand gene–environment interactions and support breeding for improved resource use efficiency of important crops such as bean (Phaseolus vulgaris L.). Here we provide an overview of state-of-the-art phenotyping approaches addressing three aspects of resource use efficiency in plants: belowground roots, aboveground shoots and transport/allocation processes. We demonstrate the capacity of high-precision methods to measure plant function or structural traits non-invasively, stating examples wherever possible. Ideally, high-precision methods are complemented by fast and high-throughput technologies. High-throughput phenotyping can be applied in the laboratory using automated data acquisition, as well as in the field, where imaging spectroscopy opens a new path to understand plant function non-invasively. For example, we demonstrate how magnetic resonance imaging (MRI) can resolve root structure and separate root systems under resource competition, how automated fluorescence imaging (PAM fluorometry) in combination with automated shape detection allows for high-throughput screening of photosynthetic traits and how imaging spectrometers can be used to quantify pigment concentration, sun-induced fluorescence and potentially photosynthetic quantum yield. We propose that these phenotyping techniques, combined with mechanistic knowledge on plant structure–function relationships, will open new research directions in whole-plant ecophysiology and may assist breeding for varieties with enhanced resource use efficiency varieties.

scholarly journals Female dietary bias towards large migratory moths in the European free-tailed bat ( Tadarida teniotis )

2016 ◽  
Vol 12 (3) ◽  
pp. 20150988 ◽  
Author(s):  
Vanessa A. Mata ◽  
Francisco Amorim ◽  
Martin F. V. Corley ◽  
Gary F. McCracken ◽  
Hugo Rebelo ◽  
...  
Keyword(s):  
High Throughput ◽  
Resource Use ◽  
Diet Composition ◽  
High Throughput Sequencing ◽  
Food Resource ◽  
Migratory Behaviour ◽  
Molecular Tools ◽  
Autographa Gamma ◽  
Males And Females ◽  
Dietary Variation

In bats, sexual segregation has been described in relation to differential use of roosting and foraging habitats. It is possible that variation may also exist between genders in the use of different prey types. However, until recently this idea was difficult to test owing to poorly resolved taxonomy of dietary studies. Here, we use high-throughput sequencing to describe gender-related variation in diet composition of the European free-tailed bat ( Tadarida teniotis ), while controlling for effects of age and season. We analysed guano pellets collected from 143 individuals mist-netted from April to October 2012 and 2013, in northeast Portugal. Moths (Lepidoptera; mainly Noctuidae and Geometridae) were by far the most frequently recorded prey, occurring in nearly all samples and accounting for 96 out of 115 prey taxa. There were significant dietary differences between males and females, irrespective of age and season. Compared to males, females tended to consume larger moths and more moths of migratory behaviour (e.g. Autographa gamma ). Our study provides the first example of gender-related dietary variation in bats, illustrating the value of novel molecular tools for revealing intraspecific variation in food resource use in bats and other insectivores.

Functional RNA microarrays for high-throughput screening of antiprotein aptamers

2005 ◽  
Vol 338 (1) ◽  
pp. 113-123 ◽  
Author(s):  
James R. Collett ◽  
Eun Jeong Cho ◽  
Jennifer F. Lee ◽  
Matthew Levy ◽  
Allysia J. Hood ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Rna Microarrays ◽  
Functional Rna

LA-ICP-TOF-MS for rapid, all-elemental and quantitative bioimaging, isotopic analysis and the investigation of plasma processes

2019 ◽  
Vol 34 (4) ◽  
pp. 694-701 ◽  
Author(s):  
Oliver Bolle Bauer ◽  
Oliver Hachmöller ◽  
Olga Borovinskaya ◽  
Michael Sperling ◽  
Hans-Joachim Schurek ◽  
...  
Keyword(s):  
High Throughput ◽  
Spectral Imaging ◽  
Isotopic Analysis ◽  
Imaging Approach ◽  
Tof Ms ◽  
Rat Kidneys

This work presents a high-throughput and quasisimultaneous, full-spectral imaging approach for the analysis of cisplatin perfused rat kidneys using LA-ICP-TOF-MS.

Laboratory and in-situ analysis of comet dust

1988 ◽  
Vol 46 ◽  
pp. 8-9
Author(s):  
D.E. Brownlee ◽  
A.L. Albee
Keyword(s):  
Electron Microscopy ◽  
Solar System ◽  
Laboratory Study ◽  
Isotopic Analysis ◽  
Building Blocks ◽  
Sem Analysis ◽  
Early Solar System ◽  
Cometary Material ◽  
Comet Dust

Comets are primitive, kilometer-sized bodies that formed in the outer regions of the solar system. Composed of ice and dust, comets are generally believed to be relic building blocks of the outer solar system that have been preserved at cryogenic temperatures since the formation of the Sun and planets. The analysis of cometary material is particularly important because the properties of cometary material provide direct information on the processes and environments that formed and influenced solid matter both in the early solar system and in the interstellar environments that preceded it.The first direct analyses of proven comet dust were made during the Soviet and European spacecraft encounters with Comet Halley in 1986. These missions carried time-of-flight mass spectrometers that measured mass spectra of individual micron and smaller particles. The Halley measurements were semi-quantitative but they showed that comet dust is a complex fine-grained mixture of silicates and organic material. A full understanding of comet dust will require detailed morphological, mineralogical, elemental and isotopic analysis at the finest possible scale. Electron microscopy and related microbeam techniques will play key roles in the analysis. The present and future of electron microscopy of comet samples involves laboratory study of micrometeorites collected in the stratosphere, in-situ SEM analysis of particles collected at a comet and laboratory study of samples collected from a comet and returned to the Earth for detailed study.

Differences in productivity QOL, and resource use in newly diagnosed and established irritable bowel syndrome (IBS) patients

2001 ◽  
Vol 120 (5) ◽  
pp. A634-A634
Author(s):  
P JHINGRAN ◽  
J RICCI ◽  
M MARKOWITZ ◽  
S GORDON ◽  
A ASGHARIAN ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Resource Use ◽  
Newly Diagnosed ◽  
Irritable Bowel

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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