scholarly journals TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells

2016 ◽  
Vol 8 (2) ◽  
pp. 110-116 ◽  
Author(s):  
Li-Jia Rao ◽  
Bai-Cheng Yi ◽  
Qi-Meng Li ◽  
Qiong Xu
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals Odontogenic differentiation potential of human dental pulp cells cultured on a calcium-aluminate enriched chitosan-collagen scaffold

2017 ◽  
Vol 21 (9) ◽  
pp. 2827-2839 ◽  
Author(s):  
Diana Gabriela Soares ◽  
Hebert Luís Rosseto ◽  
Débora Salles Scheffel ◽  
Fernanda Gonçalves Basso ◽  
Claudia Huck ◽  
...  
Keyword(s):  
Calcium Aluminate ◽  
Dental Pulp ◽  
Collagen Scaffold ◽  
Dental Pulp Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured

scholarly journals Sclerostin promotes human dental pulp cells senescence

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5808 ◽  
Author(s):  
Yanjing Ou ◽  
Yi Zhou ◽  
Shanshan Liang ◽  
Yining Wang
Keyword(s):  
Dental Pulp ◽  
Wnt Signaling Pathway ◽  
Dental Pulp Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp Cells ◽  
Qrt Pcr ◽  
Odontoblastic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Background Senescence-related impairment of proliferation and differentiation limits the use of dental pulp cells for tissue regeneration. Deletion of sclerostin improves the dentinogenesis regeneration, while its role in dental pulp senescence is unclear. We investigated the role of sclerostin in subculture-induced senescence of human dental pulp cells (HDPCs) and in the senescence-related decline of proliferation and odontoblastic differentiation. Methods Immunohistochemical staining and qRT-PCR analyses were performed to examine the expression pattern of sclerostin in young (20–30-year-old) and senescent (45–80-year-old) dental pulps. HDPCs were serially subcultured until senescence, and the expression of sclerostin was examined by qRT-PCR analysis. HDPCs with sclerostin overexpression and knockdown were constructed to investigate the role of sclerostin in HDPCs senescence and senescence-related impairment of odontoblastic differentiation potential. Results By immunohistochemistry and qRT-PCR, we found a significantly increased expression level of sclerostin in senescent human dental pulp compared with that of young human dental pulp. Additionally, elevated sclerostin expression was found in subculture-induced senescent HDPCs in vitro. By sclerostin overexpression and knockdown, we found that sclerostin promoted HDPCs senescence-related decline of proliferation and odontoblastic differentiation potential with increased expression of p16, p53 and p21 and downregulation of the Wnt signaling pathway. Discussion The increased expression of sclerostin is responsible for the decline of proliferation and odontoblastic differentiation potential of HDPCs during cellular senescence. Anti-sclerostin treatment may be beneficial for the maintenance of the proliferation and odontoblastic differentiation potentials of HDPCs.

scholarly journals Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

2020 ◽  
Vol 2020 ◽  
pp. 1-18 ◽  
Author(s):  
Xiangfen Li ◽  
Liu Wang ◽  
Qin Su ◽  
Ling Ye ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Dental Pulp ◽  
Biological Behavior ◽  
Dental Pulp Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp Cells ◽  
Beta Tricalcium Phosphate ◽  
Human Dental Pulp ◽  
Pulp Cells

Human dental pulp cells (HDPCs) play a vital role in dentin formation and reparative dentinogenesis, which indicated their potential application in regenerative medicine. However, HDPCs, which can only be obtained from scarce human pulp tissues, also have a limited lifespan in vitro, and stem cells usually lose their original characteristics over a large number of passages. To overcome these challenges, we successfully immortalized human dental pulp cells using the piggyBac system which was employed to efficiently overexpress the SV40 T-Ag, and we then comprehensively described the cell biological behavior. The immortalized human dental pulp cells (iHDPCs) acquired long-term proliferative activity and expressed most HDPC markers. The iHDPCs maintained multiple differentiation potential and could be induced to differentiate into chondrogenic, osteogenic, and adipogenic cells in vitro. We also proved that the iHDPCs gained a stronger ability to migrate than the primary cells, while apoptosis was inhibited. Furthermore, highly proliferative iHDPCs displayed no oncogenicity when subcutaneously implanted into athymic nude mice. Finally, iHDPCs exhibited odontogenic differentiation ability and secreted dentin sialophosphoprotein (DSPP) when combined with a beta-tricalcium phosphate scaffold and bone morphogenetic protein-2 (BMP2) in vivo. Conclusively, the established iHDPCs are a valuable resource for mechanistic study of dental pulp cell differentiation and dental pulp injury repair, as well as for applications in tooth regeneration.

scholarly journals Melatonin-induced suppression of DNA methylation promotes odontogenic differentiation in human dental pulp cells

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 829-840
Author(s):  
Jingzhou Li ◽  
Qianyi Deng ◽  
Wenguo Fan ◽  
Qi Zeng ◽  
Hongwen He ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Biosilica Porous Microspheres Promote the Osteogenic/Odontogenic Differentiation of Human Dental Pulp Cells

2018 ◽  
Vol 8 (2) ◽  
pp. 258-266 ◽  
Author(s):  
Wenhao Zhu ◽  
Xuejun Gao ◽  
Xiaoying Zou ◽  
Werner E. G. Müller ◽  
Shunfeng Wang ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Porous Microspheres ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

Effect of 5-Aza-2′-deoxycytidine on Odontogenic Differentiation of Human Dental Pulp Cells

2015 ◽  
Vol 41 (5) ◽  
pp. 640-645 ◽  
Author(s):  
Deqian Zhang ◽  
Qimeng Li ◽  
Lijia Rao ◽  
Baicheng Yi ◽  
Qiong Xu
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals Effect of histone demethylase KDM5A on the odontogenic differentiation of human dental pulp cells

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 449-462 ◽  
Author(s):  
Qi-Meng Li ◽  
Jin-Ling Li ◽  
Zhi-Hui Feng ◽  
Huan-Cai Lin ◽  
Qiong Xu
Keyword(s):  
Dental Pulp ◽  
Histone Demethylase ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells
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