scholarly journals Chondrogenesis of periodontal ligament stem cells by transforming growth factor-β3 and bone morphogenetic protein-6 in a normal healthy impacted third molar

2013 ◽  
Vol 5 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Sunyoung Choi ◽  
Tae-Jun Cho ◽  
Soon-Keun Kwon ◽  
Gene Lee ◽  
Jaejin Cho
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Bone Morphogenetic Protein ◽  
Periodontal Ligament ◽  
Transforming Growth Factor ◽  
Third Molar ◽  
Periodontal Ligament Stem Cells ◽  
Impacted Third Molar ◽  
Morphogenetic Protein ◽  
Bone Morphogenetic Protein 6

scholarly journals Transforming Growth Factor-β3/Chitosan Sponge (TGF-β3/CS) Facilitates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

2019 ◽  
Vol 20 (20) ◽  
pp. 4982 ◽  
Author(s):  
Yangfan Li ◽  
Zhifen Qiao ◽  
Fenglin Yu ◽  
Huiting Hu ◽  
Yadong Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Periodontal Disease ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Transforming Growth Factor ◽  
Calcium Content ◽  
Hard Tissue ◽  
Periodontal Ligament Stem Cells ◽  
Tissue Defects

Periodontal disease is the main reason for tooth loss in adults. Tissue engineering and regenerative medicine are advanced technologies used to manage soft and hard tissue defects caused by periodontal disease. We developed a transforming growth factor-β3/chitosan sponge (TGF-β3/CS) to repair periodontal soft and hard tissue defects. We investigated the proliferation and osteogenic differentiation behaviors of primary human periodontal ligament stem cells (hPDLSCs) to determine the bioactivity and potential application of TGF-β3 in periodontal disease. We employed calcein-AM/propidium iodide (PI) double labeling or cell membranes (CM)-Dil labeling coupled with fluorescence microscopy to trace the survival and function of cells after implantation in vitro and in vivo. The mineralization of osteogenically differentiated hPDLSCs was confirmed by measuring alkaline phosphatase (ALP) activity and calcium content. The levels of COL I, ALP, TGF-βRI, TGF-βRII, and Pp38/t-p38 were assessed by western blotting to explore the mechanism of bone repair prompted by TGF-β3. When hPDLSCs were implanted with various concentrations of TGF-β3/CS (62.5–500 ng/mL), ALP activity was the highest in the TGF-β3 (250 ng/mL) group after 7 d (p < 0.05 vs. control). The calcium content in each group was increased significantly after 21 and 28 d (p < 0.001 vs. control). The optimal result was achieved by the TGF-β3 (500 ng/mL) group. These results showed that TGF-β3/CS promotes osteogenic differentiation of hPDLSCs, which may involve the p38 mitogen-activated protein kinase (MAPK) signaling pathway. TGF-β3/CS has the potential for application in the repair of incomplete alveolar bone defects.

scholarly journals Transforming Growth Factor-β3/Recombinant Human-like Collagen/Chitosan Freeze-Dried Sponge Primed With Human Periodontal Ligament Stem Cells Promotes Bone Regeneration in Calvarial Defect Rats

2021 ◽  
Vol 12 ◽  
Author(s):  
Shiyi Huang ◽  
Fenglin Yu ◽  
Yating Cheng ◽  
Yangfan Li ◽  
Yini Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Transforming Growth Factor ◽  
Bone Volume ◽  
Calvarial Defect ◽  
Skull Defect ◽  
Periodontal Ligament Stem Cells ◽  
Freeze Dried

Patients with a skull defect are at risk of developing cerebrospinal fluid leakage and ascending bacterial meningitis at &gt;10% per year. However, treatment with stem cells has brought great hope to large-area cranial defects. Having found that transforming growth factor (TGF)-β3 can promote the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs), we designed a hybrid TGF-β3/recombinant human-like collagen recombinant human collagen/chitosan (CS) freeze-dried sponge (TRFS) loading hPDLSCs (TRFS-h) to repair skull defects in rats. CFS with 2% CS was selected based on the swelling degree, water absorption, and moisture retention. The CS freeze-dried sponge (CFS) formed a porous three-dimensional structure, as observed by scanning electron microscopy. In addition, cytotoxicity experiments and calcein-AM/PI staining showed that TRFS had a good cellular compatibility and could be degraded completely at 90 days in the implantation site. Furthermore, bone healing was evaluated using micro-computed tomography in rat skull defect models. The bone volume and bone volume fraction were higher in TRFS loaded with hPDLSCs (TRFS-h) group than in the controls (p &lt; 0.01, vs. CFS or TRFS alone). The immunohistochemical results indicated that the expression of Runx2, BMP-2, and collagen-1 (COL Ⅰ) in cells surrounding bone defects in the experimental group was higher than those in the other groups (p &lt; 0.01, vs. CFS or TRFS alone). Taken together, hPDLSCs could proliferate and undergo osteogenic differentiation in TRFS (p &lt; 0.05), and TRFS-h accelerated bone repair in calvarial defect rats. Our research revealed that hPDLSCs could function as seeded cells for skull injury, and their osteogenic differentiation could be accelerated by TGF-β3. This represents an effective therapeutic strategy for restoring traumatic defects of the skull.

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