scholarly journals DNA yield and quality of saliva samples and suitability for large-scale epidemiological studies in children

2011 ◽  
Vol 35 (S1) ◽  
pp. S113-S118 ◽  
Author(s):  
A C Koni ◽  
◽  
R A Scott ◽  
G Wang ◽  
M E S Bailey ◽  
...  
Keyword(s):  
Large Scale ◽  
Epidemiological Studies ◽  
Dna Yield ◽  
Yield And Quality

scholarly journals Scientific and practical fundamentals of growing cabbage (Brassica capitata lizg.) in Uzbekistan

2021 ◽  
Vol 939 (1) ◽  
pp. 012044
Author(s):  
A J Shokirov ◽  
S S Lapasov ◽  
K J Shokirov
Keyword(s):  
Large Scale ◽  
Scientific Research ◽  
Mineral Fertilizers ◽  
Biological Productivity ◽  
High Quality ◽  
Planting Time ◽  
Yield And Quality ◽  
White Cabbage ◽  
Urgent Task

Abstract At present, scientific research is underway to further develop vegetable growing in the secondary crop, in particular to further increase the yield and quality of white cabbage, to select a system of planting time-sowing scheme that maximizes the biological productivity of varieties, and to apply the most optimal standards of fertilization and irrigation. In this regard, the urgent task remains to determine the optimal varieties of cabbage that can be grown in repeated crops, their optimal planting scheme, timing, development and implementation of optimal standards for each variety of mineral fertilizers and irrigation, and its solution is large-scale throughout the country. Besides that a number of problematic issues are addressed, which could allow to get high and high-quality harvest of white cabbage in repeated sowing in grain-free areas.

scholarly journals The EMBARC European Bronchiectasis Registry: protocol for an international observational study

2016 ◽  
Vol 2 (1) ◽  
pp. 00081-2015 ◽  
Author(s):  
James D. Chalmers ◽  
Stefano Aliberti ◽  
Eva Polverino ◽  
Montserrat Vendrell ◽  
Megan Crichton ◽  
...  
Keyword(s):  
Observational Study ◽  
Large Scale ◽  
Research Collaboration ◽  
Clinical History ◽  
Epidemiological Studies ◽  
Annual Reports ◽  
Neglected Diseases ◽  
History Of ◽  
Randomised Controlled

Bronchiectasis is one of the most neglected diseases in respiratory medicine. There are no approved therapies and few large-scale, representative epidemiological studies.The EMBARC (European Multicentre Bronchiectasis Audit and Research Collaboration) registry is a prospective, pan-European observational study of patients with bronchiectasis. The inclusion criterion is a primary clinical diagnosis of bronchiectasis consisting of: 1) a clinical history consistent with bronchiectasis; and 2) computed tomography demonstrating bronchiectasis. Core exclusion criteria are: 1) bronchiectasis due to known cystic fibrosis; 2) age <18 years; and 3) patients who are unable or unwilling to provide informed consent.The study aims to enrol 1000 patients by April 2016 across at least 20 European countries, and 10 000 patients by March 2020. Patients will undergo a comprehensive baseline assessment and will be followed up annually for up to 5 years with the goal of providing high-quality longitudinal data on outcomes, treatment patterns and quality of life. Data from the registry will be available in the form of annual reports. and will be disseminated in conference presentations and peer-reviewed publications.The European Bronchiectasis Registry aims to make a major contribution to understanding the natural history of the disease, as well as guiding evidence-based decision making and facilitating large randomised controlled trials.

scholarly journals Role of biobanking in managing large-scale epidemiological studies

2021 ◽  
Vol 20 (5) ◽  
pp. 2958
Author(s):  
M. S. Pokrovskaya ◽  
A. L. Borisova ◽  
V. A. Metelskaya ◽  
I. A. Efimova ◽  
Yu. V. Doludin ◽  
...  
Keyword(s):  
Blood Serum ◽  
Whole Blood ◽  
Large Scale ◽  
Venous Blood ◽  
Epidemiological Studies ◽  
National Medical ◽  
And Storage ◽  
Large Scale Epidemiological Study

The success and quality of large-scale epidemiological studies depends entirely on biomaterial quality. Therefore, when arranging the third Epidemiology of Cardiovascular Diseases and their Risk Factors in Regions of Russian Federation (ESSE-RF-3) study, increased attention was paid to specifics of collection, processing and further transportation of biological samples and related clinical and anthropometric data of participants from regional collection centers to Biobank.Aim. To develop a methodology for collection of high-quality biomaterials within the large-scale epidemiological study, involving the sampling, processing, freezing of blood and its derivatives (serum, plasma) in the regions, followed by transportation and storage of obtained biomaterial in the Biobank of National Medical Research Center for Therapy and Preventive Medicine (Moscow).Material and methods. To conduct the ESSE-RF-3 study, a design was developed, according to which the collection of venous blood samples in a total volume of 29,5 ml from each participant is planned in all participating regions in order to obtain and store samples of whole blood, serum and two types of plasma.Results. On the basis of international biobanking standards, ethical norms, experience from ESSE-RF and ESSE-RF-2, and literature data, a protocol for biobanking of blood and its derivatives was developed. The type and number of serum and plasma aliquots obtained, the required standard technical means and consumables, as well as logistic biomaterial requirements were determined. Training programs for regional participants were developed. By the beginning of August 2021, 180 thousand samples of whole blood, serum and plasma from more than 23 thousand participants from 28 Russian regions were collected, processed and stored.Conclusion. The presented work made it possible to assess and confirm the compliance of developed biobanking protocol with quality requirements. However, due to the coronavirus disease 2019 pandemic, by August 2021, the Biobank did not reach the maximum effectiveness predicted for the ESSE-RF-3 project.

scholarly journals EVALUATION OF BIVOLTINE P1 SEED COCOONS GENERATION AND PRODUCED COMMERCIAL BIVOLTINE HYBRID DFLS AT SSPC VIJAYAPURA

2020 ◽  
pp. 1-2
Author(s):  
Dr. P. M. Muniswamy Reddy* ◽  
M. Venkatesh ◽  
R.k. Mishra
Keyword(s):  
Large Scale ◽  
Intervention Strategy ◽  
Total Production ◽  
Mulberry Leaf ◽  
Yield And Quality ◽  
Leaf Yield ◽  
Adoption Level ◽  
Rural Industries ◽  
Selection Of

Selection of P1 seed farmers (ASRs) for different combination of P1 seed cocoon generation and supply to SSPC Vijayapura to produced large scale commercial double hybrid and SK hybrid ds. Sericulture is one of the most important rural industries practiced since several decades. It has certain inherent level to educate Sericulturists, still a wide gap exists between there commended technology and actual adoption by Sericulturists. To plan a suitable intervention strategy, to bridge this gap, it is necessary to understand the present knowledge and adoption level for improved technologies, so also existing mulberry leaf yield and quality seed cocoon generation. Therefore, present study was conducted to know the extent of adoption of improved practices at farmer's level in selected areas of ASR farmers Doddaballapura, Koratagere, Nelamangala and Siddlaghatta covering ve locations with purpose of good quality of seed cocoons generation and to produce quality ds. During the year 2018-19 signicant achievement of SSPC Vijayapura Production of Bivoltine F1 Silkworm Eggs on large scale as per the annual targets of 25 lakhs this centre could record a total production a quantity of 38,40,050 lakhs Bivoltine F1 ds was produced in different combination month wise and ds recovery Table-1&2.

scholarly journals Collection of Human Genomic DNA From Buccal Cells for Genetics Studies: Comparison Between Cytobrush, Mouthwash, and Treated Card

2005 ◽  
Vol 2005 (3) ◽  
pp. 291-296 ◽  
Author(s):  
Claire Mulot ◽  
Isabelle Stücker ◽  
Jacqueline Clavel ◽  
Philippe Beaune ◽  
Marie-Anne Loriot
Keyword(s):  
Large Scale ◽  
Storage Conditions ◽  
Sample Collection ◽  
Buccal Cells ◽  
Genetic Studies ◽  
Dna Yield ◽  
Dna Collection ◽  
Alternative Sources ◽  
And Storage

Alternative sources such as buccal cells have already been tested for genetic studies and epidemiological investigations. Thirty-seven volunteers participated in this study to compare cytology brushes, mouthwash, and treated cards for DNA collection. Quantity and quality of DNA and cost and feasibility were assessed. The mean DNA yield at 260 nm was found to be3.5,4, and2.6μg for cytobrushes, mouthwashes, and treated cards, respectively. A second quantification technique by fluorescence showed differences in the DNA yield with1.1and5.2μg for cytobrushes and mouthwash, respectively. All buccal samples allowed isolation of DNA suitable for polymerase chain reaction. According to the procedure of sample collection, the yield and purity of collected DNA, and storage conditions, the use of cytobrush appears to be the more appropriate method for DNA collection. This protocol has been validated and is currently applied in three large-scale multicentric studies including adults or children.

scholarly journals Vitamin D deficiency in Russia: the first results of a registered, non-interventional study of the frequency of vitamin D deficiency and insufficiency in various geographic regions of the country

2021 ◽  
Vol 67 (2) ◽  
pp. 84-92
Author(s):  
V. A. Avdeeva ◽  
L. A. Suplotova ◽  
E. A. Pigarova ◽  
L. Y. Rozhinskaya ◽  
E. A. Troshina
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Russian Federation ◽  
Large Scale ◽  
Epidemiological Studies ◽  
Cross Sectional ◽  
First Results ◽  
Wide Scale ◽  
The Russian Federation

BACKGROUND. In Russian Federation, there are no large-scale cross-sectional multicenter epidemiological studies assessing the prevalence of vitamin D deficiency and insufficiency in different geographical latitudes. Insufficient solar insolation and inadequate vitamin D content in food dictate the need to study the epidemiological structure of low vitamin D status in Russia.AIM. To assess the incidence of vitamin D deficiency and insufficiency among the population living in the regions of the Russian Federation located at latitudes from 45 ° to 70 °.MATERIALS AND METHODS. The first stage of the Russian multicenter non-interventional registry study using the «crosssectional» method was carried out from March 2020 to May 2020.RESULTS. In regions that represent a geographically representative sample of regions of the Russian Federation with a high risk of developing low levels of vitamin D, it’s deficiency was noted in 55.96%, and the level of deficiency and insufficiency was recorded in 84.01%.CONCLUSION. Close attention to the wide scale of the problem of vitamin D deficiency and insufficiency in the Russian Federation will contribute to the progressive formation of various educational and preventive programs necessary to strengthen health and improve the quality of life of the population.

Evolution of yield and quality of sainfoin (Onobrychis viciifolia Scop.) in the spring growth cycle

Agronomie ◽  
2003 ◽  
Vol 23 (3) ◽  
pp. 193-201 ◽  
Author(s):  
Giorgio Borreani ◽  
Pier Giorgio Peiretti ◽  
Ernesto Tabacco
Keyword(s):  
Growth Cycle ◽  
Onobrychis Viciifolia ◽  
Yield And Quality

Assessment of quality of life in patients with the effects of transient ischemic stroke

2016 ◽  
pp. 37-39
Author(s):  
A. Babirad
Keyword(s):  
Quality Of Life ◽  
Ischemic Stroke ◽  
Large Scale ◽  
Cerebrovascular Diseases ◽  
Control Measures ◽  
Sociological Research ◽  
Cerebrovascular Events ◽  
Sf 36 ◽  
Angioplasty And Stenting

Cerebrovascular diseases are a problem of the world today, and according to the forecast, the problem of the near future arises. The main risk factors for the development of ischemic disorders of the cerebral circulation include oblique and aging, arterial hypertension, smoking, diabetes mellitus and heart disease. An effective strategy for the prevention of cerebrovascular events is based on the implementation of large-scale risk control measures, including the use of antiagregant and anticoagulant therapy, invasive interventions such as atheromectomy, angioplasty and stenting. In this connection, the efforts of neurologists, cardiologists, angiosurgery, endocrinologists and other specialists are the basis for achieving an acceptable clinical outcome. A review of the SF-36 method for assessing the quality of life in patients with the effects of transient ischemic stroke is presented. The assessment of quality of life is recognized in world medical practice and research, an indicator that is also used to assess the quality of the health system and in general sociological research.

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