Response of peripheral blood leucocytes to mitogenic factor(s) in porcine seminal plasma

1994 ◽  
Vol 72 (2) ◽  
pp. 129-135 ◽  
Author(s):  
D. PAUL R. KOVACS ◽  
FRANCIS R. TEKPETEY ◽  
DAVID T. ARMSTRONG
Keyword(s):  
Seminal Plasma ◽  
Peripheral Blood ◽  
Mitogenic Factor ◽  
Peripheral Blood Leucocytes

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Effects of the antiprotease Trasylol® on peripheral blood leucocytes

1978 ◽  
Vol 34 (4) ◽  
pp. 528-530 ◽  
Author(s):  
A. W. Thomson ◽  
R. G. P. Pugh-Humphreys ◽  
D. J. Tweedie ◽  
C. H. W. Horne
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Leucocytes

An improved immunocytochemical method for the detection of human cytomegalovirus antigens in peripheral blood leucocytes

1989 ◽  
Vol 91 (4) ◽  
pp. 345-349 ◽  
Author(s):  
N. M. Jiwa ◽  
F. M. van de Rijke ◽  
A. Mulder ◽  
W. van der Bij ◽  
T. H. The ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Peripheral Blood ◽  
Immunocytochemical Method ◽  
Peripheral Blood Leucocytes

scholarly journals Zinc supplementation has no effect on circulating levels of peripheral blood leucocytes and lymphocyte subsets in healthy adult men

2003 ◽  
Vol 89 (5) ◽  
pp. 695-703 ◽  
Author(s):  
Maxine Bonham ◽  
Jacqueline M. O'Connor ◽  
H. Denis Alexander ◽  
James Coulter ◽  
Paula M. Walsh ◽  
...  
Keyword(s):  
T Cells ◽  
Adverse Effects ◽  
Seasonal Variations ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Immune Status ◽  
Control Group ◽  
Dietary Intakes ◽  
Circulating Levels ◽  
Peripheral Blood Leucocytes

As a result of evidence documenting harmful effects of Zn supplementation on immune function and Cu status, thirty-eight men were recruited onto a Zn supplementation trial. The aim was to examine the effects of chronic Zn supplementation on circulating levels of peripheral blood leucocytes and lymphocyte subsets. Subjects (n 19) took 30 mg Zn/d for 14 weeks followed by 3 mg Cu/d for 8 weeks to counteract adverse effects, if any, of Zn supplementation on immune status resulting from lowered Cu status. A control group (n 19) took placebo supplements for the duration of the trial. Dietary intakes of Zn approximated 10 mg/d. Blood samples, taken throughout the trial, were assessed for full blood profiles and flow cytometric analyses of lymphocyte subsets. Putative indices of Cu status were also examined. Results indicate that there was no effect of Zn supplementation on circulating levels of peripheral blood leucocytes or on lymphocyte subsets. Cu status was also unaltered. Independent of supplement, there appeared to be seasonal variations in selected lymphocyte subsets in both placebo and supplemented groups. Alterations in circulating levels of B cells (cluster of differentiation (CD) 19), memory T cells (CD45RO) and expression of the intracellular adhesion molecule-1 (CD54) on T cells were observed. Findings indicated no adverse effects of Zn supplementation on immune status or Cu status and support the US upper level of Zn tolerance of 40 mg/d. The seasonal variations observed in lymphocyte subsets in the group as a whole could have implications for seasonal variability in the incidence of infectious diseases.

Failure to Detect Measles Virus Rna, by Reverse Transcription-Polymerase Chain Reaction, in Peripheral Blood Leucocytes of Patients with Multiple Sclerosis

1996 ◽  
Vol 1 (4) ◽  
pp. 204-206 ◽  
Author(s):  
B. Brankin ◽  
M. Osman ◽  
L. Herlihy ◽  
S.A. Hawkins ◽  
S.L. Cosby
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Peripheral Blood ◽  
Measles Virus ◽  
Chain Reaction ◽  
Specific Primers ◽  
Polymerase Chain ◽  
Multiple Sclerosis Patients ◽  
Peripheral Blood Leucocytes

We have examined peripheral blood leucocytes (PBLs) from 17 multiple sclerosis patients, two patients with rheumatoid arthritis, one case of acute childhood measles and one case of subacute sclerosing panencephalitis, as well as 19 healthy adult controls for measles virus (MV) RNA, by the technique of reverse transcription-polymerase chain reaction. MV nucleocapsid gene specific primers were used to amplify all PBL-derived cDNA samples. These proved to be negative with the exception of the sample derived from the acute measles case. Selected cases were examined further, using fusion gene and matrix gene specific primers. MV RNA could not be detected.

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