Clonal analysis of the effect of iron on human cytotoxic and proliferating T lymphocytes

1990 ◽  
Vol 68 (5) ◽  
pp. 317-324 ◽  
Author(s):  
Michael J. Rudd ◽  
Michael F. Good ◽  
D. E. Chapman ◽  
L. W. Powell ◽  
J. W. Halliday
Keyword(s):  
T Lymphocytes ◽  
Clonal Analysis

scholarly journals Phenotypic and clonal analysis of T lymphocytes in childhood immune thrombocytopenic purpura

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2137-2142 ◽  
Author(s):  
RE Ware ◽  
TA Howard
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Immune Thrombocytopenic Purpura ◽  
Clonal Analysis ◽  
In Vitro Proliferation ◽  
Cell Reactivity ◽  
Thrombocytopenic Purpura ◽  
Chronic Itp ◽  
Age Related

Abstract In an attempt to identify and characterize T-lymphocyte immunoregulatory abnormalities in immune thrombocytopenic purpura (ITP), we have performed phenotypic and clonal analysis on peripheral T lymphocytes from 23 children with ITP. Quantitation of lymphocyte subpopulations showed that children with acute ITP had higher numbers of CD45RA+ and lower numbers of CD45RO+ T cells than children with chronic ITP or controls, but these differences may be age related. Analysis of T-cell receptor variable beta gene usage identified 2 boys with chronic ITP and elevated numbers of V beta 8+ T cells. Eight T- cell clones were established (6 CD4+, 4B4+ helper-inducer lines and 2 CD8+ lines) that showed in vitro proliferation against allogeneic platelets. The addition of autologous antigen-presenting cells enhanced the proliferation of six clones, but not for two clones that coexpressed natural killer (NK) markers. Four of seven positive clones also had measurable interleukin (IL)-2 secretion following platelet stimulation, providing further evidence for T-cell reactivity. Our results provide the first evidence that patients with ITP may have platelet-reactive T lymphocytes identifiable at the clonal level, supporting the hypothesis that autoreactive peripheral T lymphocytes may mediate or participate in the pathogenesis of this disorder.

Clonal analysis of cytotoxic T lymphocytes (CTL) against autologous melanoma

1986 ◽  
Vol 22 (1) ◽  
Author(s):  
Peter Hersey ◽  
Malcolm MacDonald ◽  
Stephen Schibeci ◽  
Christine Burns
Keyword(s):  
T Lymphocytes ◽  
Cytotoxic T Lymphocytes ◽  
Clonal Analysis

scholarly journals Phenotypic and clonal analysis of T lymphocytes in childhood immune thrombocytopenic purpura

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2137-2142 ◽  
Author(s):  
RE Ware ◽  
TA Howard
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Lymphocytes ◽  
Immune Thrombocytopenic Purpura ◽  
Clonal Analysis ◽  
In Vitro Proliferation ◽  
Cell Reactivity ◽  
Thrombocytopenic Purpura ◽  
Chronic Itp ◽  
Age Related

In an attempt to identify and characterize T-lymphocyte immunoregulatory abnormalities in immune thrombocytopenic purpura (ITP), we have performed phenotypic and clonal analysis on peripheral T lymphocytes from 23 children with ITP. Quantitation of lymphocyte subpopulations showed that children with acute ITP had higher numbers of CD45RA+ and lower numbers of CD45RO+ T cells than children with chronic ITP or controls, but these differences may be age related. Analysis of T-cell receptor variable beta gene usage identified 2 boys with chronic ITP and elevated numbers of V beta 8+ T cells. Eight T- cell clones were established (6 CD4+, 4B4+ helper-inducer lines and 2 CD8+ lines) that showed in vitro proliferation against allogeneic platelets. The addition of autologous antigen-presenting cells enhanced the proliferation of six clones, but not for two clones that coexpressed natural killer (NK) markers. Four of seven positive clones also had measurable interleukin (IL)-2 secretion following platelet stimulation, providing further evidence for T-cell reactivity. Our results provide the first evidence that patients with ITP may have platelet-reactive T lymphocytes identifiable at the clonal level, supporting the hypothesis that autoreactive peripheral T lymphocytes may mediate or participate in the pathogenesis of this disorder.

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