scholarly journals D5 dopamine receptor decreases NADPH oxidase, reactive oxygen species and blood pressure via heme oxygenase-1

2013 ◽  
Vol 36 (8) ◽  
pp. 684-690 ◽  
Author(s):  
Quansheng Lu ◽  
Yu Yang ◽  
Van Anthony Villar ◽  
Laureano Asico ◽  
John E Jones ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Dopamine Receptor ◽  
Heme Oxygenase ◽  
Reactive Oxygen ◽  
Heme Oxygenase 1 ◽  
Oxygen Species

Abstract 369: D5 Dopamine Receptor Decreases Reactive Oxygen Species via Heme Oxygenase-1

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Quansheng Lu ◽  
Yu Yang ◽  
Van A Villar ◽  
Laureano D Asico ◽  
John E Jones ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Reactive Oxygen Species ◽  
Protein Expression ◽  
Dopamine Receptor ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Ros Production ◽  
Oxygen Species ◽  
Wild Type ◽  
Expression And Activity

D5 dopamine receptor (D5R) knock-out mice (D5-/-) have higher blood pressure and reactive oxygen species (ROS) production than their D5R wild-type littermates (D5+/+), related in part to increased NADPH oxidase expression and activity. We tested the hypothesis that the high blood pressure and increased ROS production in D5-/- mice may also be related to decreased heme oxygenase-1 (HO-1) expression and activity. We found that renal HO-1 protein expression and HO enzyme activity were decreased (by 65% and 50%, respectively) in D5-/- relative to D5+/+ mice. A 24 h of administration of hemin, an HO-1 inducer, increased HO-1 protein expression and HO activity (6.8-fold and 1.9-fold, respectively) in D5-/- but not D5+/+ mice and normalized the increased blood pressure and ROS production in D5-/- mice. Expression of HO-1 protein and HO activity were increased (2.3-fold and 1.5-fold, respectively) in HEK cells which heterologously expressed human wild-type D5R (HEK-hD5R) but not empty vector-transfected HEK-293 cells. Fenoldopam (Fen) treatment increased HO-1 protein expression and HO activity and HO-1 and D5R co-localization and co-immunoprecipitation in HEK-hD5R cells. Basal cellular ROS production was decreased by 35% in HEK-hD5R that was abrogated with silencing of the HO-1 gene. HO-1 siRNA also decreased the ability of Fen to decrease ROS production in HEK-hD5R cells. In summary, the D5R positively regulates HO-1 activity through direct protein/protein interaction in the short-term and by increasing HO-1 protein expression, in the long-term. Impaired D5R regulation of HO-1 may be involved in the pathogenesis and maintenance of hypertension in D5-/- mice.

Cytotoxic effects of metal protoporphyrins in glioblastoma cells: Roles of albumin, reactive oxygen species, and heme oxygenase-1

2008 ◽  
Vol 177 (2) ◽  
pp. 97-107 ◽  
Author(s):  
Jyh-Ming Chow ◽  
Guan-Cheng Huang ◽  
Hui-Yi Lin ◽  
Shing-Chuan Shen ◽  
Liang-Yo Yang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Heme Oxygenase ◽  
Reactive Oxygen ◽  
Heme Oxygenase 1 ◽  
Oxygen Species ◽  
Glioblastoma Cells ◽  
Cytotoxic Effects

scholarly journals 15-Deoxy-Δ12,14-prostaglandin J2Induces Heme Oxygenase-1 Gene Expression in a Reactive Oxygen Species-dependent Manner in Human Lymphocytes

2004 ◽  
Vol 279 (21) ◽  
pp. 21929-21937 ◽  
Author(s):  
Moisés Álvarez-Maqueda ◽  
Rajaa El Bekay ◽  
Gonzalo Alba ◽  
Javier Monteseirín ◽  
Pedro Chacón ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Heme Oxygenase ◽  
Human Lymphocytes ◽  
Reactive Oxygen ◽  
Heme Oxygenase 1 ◽  
Dependent Manner ◽  
Oxygen Species

P-39 Anti-thrombotic properties of heme oxygenase-1 via the suppression of reactive oxygen species

2002 ◽  
Vol 34 (10) ◽  
pp. A34
Author(s):  
Hayato Matsumoto ◽  
Kazunobu Ishikawa ◽  
Keiichi Kawamura ◽  
Yasuyuki Watanabe ◽  
Yukio Maruyama
Keyword(s):  
Reactive Oxygen Species ◽  
Heme Oxygenase ◽  
Reactive Oxygen ◽  
Heme Oxygenase 1 ◽  
Oxygen Species
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