scholarly journals Inhibitory effect of ETB receptor on Na+–K+ ATPase activity by extracellular Ca2+ entry and Ca2+ release from the endoplasmic reticulum in renal proximal tubule cells

2009 ◽  
Vol 32 (10) ◽  
pp. 846-852 ◽  
Author(s):  
Yan Liu ◽  
Jian Yang ◽  
Hongmei Ren ◽  
Duofen He ◽  
Annabelle Pascua ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Atpase Activity ◽  
Proximal Tubule ◽  
Inhibitory Effect ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Etb Receptor ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells

Abstract 221: Lost Adiponectin-induced Renal Sodium Excretion in Hypertension

Hypertension ◽  
2014 ◽  
Vol 64 (suppl_1) ◽  
Author(s):  
hefei Huang ◽  
Yu Han ◽  
Zhen Wang ◽  
chunyu Zeng
Keyword(s):  
Atpase Activity ◽  
Proximal Tubule ◽  
Sodium Excretion ◽  
Inhibitory Effect ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Wistar Kyoto Rats ◽  
Tubule Cells ◽  
Wistar Kyoto ◽  
Renal Proximal Tubule Cells

Obesity related hypertensive patients are often with impaired sodium excretion. However,the mechanisms are not clear. Adipocytes secrete numerous hormones, among which adiponectin is an important one. Adiponectin KO mice developed hypertension when maintained on a high-salt diet. In hypertensive patients, the plasma adiponectin levels are lower; there is relationship between lower serum adiponectin and new-onset hypertension.We hypothesize that adiponectin induces natriuresis and diuresis, the impaired adiponectin-induced sodium excretion might be involved in hypertension. Our present study found the expressions of both adiponectin (AdipoR1 and AdipoR2) receptor in kidney from Wistar-Kyoto rats. Infusion of adiponectin via supra-renal artery induces natriuresis and diuresis in Wistar-Kyoto rats. Treatment with adiponectin inhibited Na + -K + -ATPase activity in renal proximal tubule cells of Wistar-Kyoto rats. The inhibitory effect was mainly via AdipoR2 receptor, because the siRNA of AdipoR2, not AdipoR1,completely blocked the effect of adiponectin. In the presence of inhibitor for AMPK (compound C) or eNOS (L-NAME), the inhibitory effect of adiponectin on Na+-K+-ATPase activity was blocked, indicating AMPK-NO pathway is involved in the signaling pathway. In spontaneous hypertension rats, adiponectin-induced natriuresis and diuresis were lost;similarly, the inhibitory effect on Na+-K+-ATPase activity was also lost in renal proximal tubule cells of spontaneously hypertension rats. The impaired adiponectin effect might be ascribed to lower AdipoR2 expression in renal proximal tubule cells od spontaneously hypertension rats, because transfected with AdipoR2 reversed the inhibitory effect on Na+-K+-ATPase activity. These datas suggested that adiponection, via AdipoR2,induces natriuresis and diuresis; the impaired adiponectin function might be involved in the pathogenesis of hypertension.

scholarly journals Signaling pathways utilized by PTH and dopamine to inhibit phosphate transport in mouse renal proximal tubule cells

2009 ◽  
Vol 296 (2) ◽  
pp. F355-F361 ◽  
Author(s):  
Rochelle Cunningham ◽  
Rajatsubhra Biswas ◽  
Marc Brazie ◽  
Deborah Steplock ◽  
Shirish Shenolikar ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Signaling Pathways ◽  
Phosphate Transport ◽  
Inhibitory Effect ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Phosphate Cotransport ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Sodium Phosphate Cotransport

The present experiments were designed to detail factors regulating phosphate transport in cultured mouse proximal tubule cells by determining the response to parathyroid hormone (PTH), dopamine, and second messenger agonists and inhibitors. Both PTH and dopamine inhibited phosphate transport by over 30%. The inhibitory effect of PTH was completely abolished in the presence of chelerythrine, a PKC inhibitor, but not by Rp-cAMP, a PKA inhibitor. By contrast, both chelerythrine and Rp-cAMP blocked the inhibitory effect of dopamine. Chelerythrine inhibited PTH-mediated cAMP accumulation but also blocked the inhibitory effect of 8-bromo-cAMP on phosphate transport. On the other hand, Rp-cAMP had no effect on the ability of DOG, a PKC activator, to inhibit phosphate transport. PD98059, an inhibitor of MAPK, had no effect on PTH- or dopamine-mediated inhibition of sodium-phosphate cotransport. Finally, compared with 8-bromo-cAMP, 8-pCPT-2′- O-Me-cAMP, an activator of EPAC, had no effect on phosphate transport. These results outline significant differences in the signaling pathways utilized by PTH and dopamine to inhibit renal phosphate transport. Our results also suggest that activation of MAPK is not critically involved in PTH- or dopamine-mediated inhibition of phosphate transport in mouse renal proximal tubule cells in culture.

Modulation of albumin-induced endoplasmic reticulum stress in renal proximal tubule cells by upregulation of mapk phosphatase-1

2013 ◽  
Vol 206 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Alejandra Gorostizaga ◽  
Maria Mercedes Mori Sequeiros García ◽  
Andrea Acquier ◽  
Natalia V. Gomez ◽  
Paula M. Maloberti ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Mapk Phosphatase ◽  
Renal Proximal Tubule Cells

PKB is a central molecule in the modulation of Na+-ATPase activity by albumin in renal proximal tubule cells

2019 ◽  
Vol 674 ◽  
pp. 108115 ◽  
Author(s):  
Diogo B. Peruchetti ◽  
Andreson C. Freitas ◽  
Vitor C. Pereira ◽  
Juliana V. Lopes ◽  
Christina M. Takiya ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Central Molecule ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells

Effects of TCDD and estradiol-17β on the proliferation and Na+/glucose cotransporter in renal proximal tubule cells

2005 ◽  
Vol 19 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ho Jae Han ◽  
Min Jin Lim ◽  
Yun Jung Lee ◽  
Eun Jung Kim ◽  
Young Jin Jeon ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Estradiol 17Β
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