scholarly journals In situ regeneration of retinal pigment epithelium by gene transfer of E2F2: a potential strategy for treatment of macular degenerations

Gene Therapy ◽  
2017 ◽  
Vol 24 (12) ◽  
pp. 810-818 ◽  
Author(s):  
D Kampik ◽  
M Basche ◽  
U F O Luhmann ◽  
K M Nishiguchi ◽  
J A E Williams ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Gene Transfer ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
In Situ Regeneration ◽  
Potential Strategy

Gene transfer of E2F2 induces in situ regeneration of retinal pigment epithelium

2016 ◽  
Vol 94 ◽  
Author(s):  
U. Luhmann ◽  
D. Kampik ◽  
K. Nishiguchi ◽  
M. Basche ◽  
A.J. Smith ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Gene Transfer ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
In Situ Regeneration

The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo

1988 ◽  
Vol 91 (2) ◽  
pp. 303-312
Author(s):  
N.M. McKechnie ◽  
M. Boulton ◽  
H.L. Robey ◽  
F.J. Savage ◽  
I. Grierson
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Cytokeratin 18 ◽  
Rpe Cells ◽  
Human Retinal Pigment Epithelium ◽  
Cytoskeletal Elements

The cytoskeletal elements of normal (in situ) and cultured human retinal pigment epithelium (RPE) were studied by a variety of immunocytochemical techniques. Primary antibodies to vimentin and cytokeratins were used. Positive immunoreactivity for vimentin was obtained with in situ and cultured material. The pattern of reactivity obtained with antisera and monoclonals to cytokeratins was more complex. Cytokeratin immunoreactivity could be demonstrated in situ and in cultured cells. The pattern of cytokeratin expression was similar to that of simple or glandular epithelia. A monoclonal antibody that specifically recognizes cytokeratin 18 identified a population of cultured RPE cells that had particularly well-defined filamentous networks within their cytoplasm. Freshly isolated RPE was cytokeratin 18 negative by immunofluorescence, but upon culture cytokeratin 18 positive cells were identifiable. Cytokeratin 18 positive cells were identified in all RPE cultures (other than early primaries), regardless of passage number, age or sex of the donor. In post-confluent cultures cytokeratin 18 cells were identified growing over cytokeratin 18 negative cells, suggesting an association of cytokeratin 18 immunoreactivity with cell proliferation. Immunofluorescence studies of retinal scar tissue from two individuals revealed the presence of numerous cytokeratin 18 positive cells. These findings indicate that RPE cells can be identified by their cytokeratin immunoreactivity and that the overt expression of cytokeratin 18 may be associated with proliferation of human RPE both in vitro and in vivo.

Five Fluorouracil Buckles and Retinal Pigment Epithelium (RPE) Proliferation In Situ

1988 ◽  
pp. 134-139 ◽  
Author(s):  
Lory C. Snady-McCoy ◽  
Sheldon M. Buzney ◽  
Salim A. Bishara ◽  
Michael William Gaynon
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment

scholarly journals Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina.

1993 ◽  
Vol 121 (2) ◽  
pp. 335-343 ◽  
Author(s):  
D Gundersen ◽  
S K Powell ◽  
E Rodriguez-Boulan
Keyword(s):  
Retinal Pigment Epithelium ◽  
Cultured Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Neural Retina ◽  
Apical Surface ◽  
Neural Cell Adhesion ◽  
Adhesion Role

The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. Strikingly, culture of RPE results in rapid redistribution of N-CAM to the basolateral surface. This is not due to an isoform shift, since the N-CAM expressed by cultured cells (140 kD) is the same as that expressed by RPE in vivo. Rather, the reversed polarity of N-CAM appears to result from the disruption of the contact between the RPE and the photoreceptors of the neural retina. We suggest that N-CAM in RPE and photoreceptors participate in these interactions.

Controlled exosome release from the retinal pigment epithelium in situ

2014 ◽  
Vol 129 ◽  
pp. 1-4 ◽  
Author(s):  
Christina J. Locke ◽  
Nicole R. Congrove ◽  
W. Michael Dismuke ◽  
Trent J. Bowen ◽  
W. Daniel Stamer ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment
Sign in / Sign up

Export Citation Format

Share Document