scholarly journals Erratum: Excision of HIV-1 DNA by gene editing: a proof-of-concept in vivo study

Gene Therapy ◽  
2016 ◽  
Vol 23 (8-9) ◽  
pp. 696-696 ◽  
Author(s):  
R Kaminski ◽  
R Bella ◽  
C Yin ◽  
J Otte ◽  
P Ferrante ◽  
...  
Keyword(s):  
Gene Editing ◽  
In Vivo Study ◽  
Proof Of Concept ◽  
Hiv 1

scholarly journals Excision of HIV-1 DNA by gene editing: a proof-of-concept in vivo study

Gene Therapy ◽  
2016 ◽  
Vol 23 (8-9) ◽  
pp. 690-695 ◽  
Author(s):  
R Kaminski ◽  
R Bella ◽  
C Yin ◽  
J Otte ◽  
P Ferrante ◽  
...  
Keyword(s):  
Gene Editing ◽  
In Vivo Study ◽  
Proof Of Concept ◽  
Hiv 1

scholarly journals Triple-Gene Therapy for Stroke: A Proof-of-Concept in Vivo Study in Rats

2018 ◽  
Vol 9 ◽  
Author(s):  
Mikhail E. Sokolov ◽  
Farid V. Bashirov ◽  
Vage A. Markosyan ◽  
Tatyana V. Povysheva ◽  
Filip O. Fadeev ◽  
...  
Keyword(s):  
Gene Therapy ◽  
In Vivo Study ◽  
Proof Of Concept

In Vivo Study on Vertical Transmission of the HIV-1 gag Gene via Mouse Oocytes

2009 ◽  
Vol 7 (5) ◽  
pp. 562-568 ◽  
Author(s):  
Yu-Sen Gao ◽  
Tian-Hua Huang ◽  
Dian Wang ◽  
Qing-Dong Xie ◽  
Xiang-Jin Kang
Keyword(s):  
Vertical Transmission ◽  
In Vivo Study ◽  
Mouse Oocytes ◽  
Hiv 1

scholarly journals Hyperpolarized 13 C Magnetic Resonance Imaging of Fumarate Metabolism by Parahydrogen‐induced Polarization: A Proof‐of‐Concept in vivo Study

ChemPhysChem ◽  
2021 ◽  
Vol 22 (10) ◽  
pp. 905-905
Author(s):  
Neil J. Stewart ◽  
Hitomi Nakano ◽  
Shuto Sugai ◽  
Mitsushi Tomohiro ◽  
Yuki Kase ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Induced Polarization ◽  
In Vivo Study ◽  
Proof Of Concept ◽  
Resonance Imaging

An in vivo study on active cytomegalovirus infection in relation to active HIV replication in HIV-1 infected drug addicts

1994 ◽  
Vol 28 (3) ◽  
pp. 287-291 ◽  
Author(s):  
T. Lazzarotto ◽  
B. Campisi ◽  
M.C. Re ◽  
F. Albertini ◽  
G. Furlini ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
In Vivo Study ◽  
Drug Addicts ◽  
Hiv Replication ◽  
Hiv 1

scholarly journals Hyperpolarized 13C MRI of Fumarate Metabolism by Parahydrogen‐induced Polarization: A Proof‐of‐Concept In Vivo Study

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Neil J. Stewart ◽  
Hitomi Nakano ◽  
Shuto Sugai ◽  
Mitsushi Tomohiro ◽  
Yuki Kase ◽  
...  
Keyword(s):  
Induced Polarization ◽  
In Vivo Study ◽  
Proof Of Concept ◽  
Hyperpolarized 13C

scholarly journals Humanized Mice for the Evaluation of Novel HIV-1 Therapies

2021 ◽  
Vol 12 ◽  
Author(s):  
Shawn Abeynaike ◽  
Silke Paust
Keyword(s):  
Gene Editing ◽  
Efficacy And Safety ◽  
Therapeutic Approaches ◽  
Humanized Mouse ◽  
The Past ◽  
Suitable Animal Model ◽  
Humanized Mouse Models ◽  
Hiv 1 ◽  
Specific Contribution

With the discovery of antiretroviral therapy, HIV-1 infection has transitioned into a manageable but chronic illness, which requires lifelong treatment. Nevertheless, complete eradication of the virus has still eluded us. This is partly due to the virus’s ability to remain in a dormant state in tissue reservoirs, ‘hidden’ from the host’s immune system. Also, the high mutation rate of HIV-1 results in escape mutations in response to many therapeutics. Regardless, the development of novel cures for HIV-1 continues to move forward with a range of approaches from immunotherapy to gene editing. However, to evaluate in vivo pathogenesis and the efficacy and safety of therapeutic approaches, a suitable animal model is necessary. To this end, the humanized mouse was developed by McCune in 1988 and has continued to be improved on over the past 30 years. Here, we review the variety of humanized mouse models that have been utilized through the years and describe their specific contribution in translating HIV-1 cure strategies to the clinic.

scholarly journals CRISPR-Cas9 as a curative drug for chronic viral infection

2021 ◽  
Vol 41 ◽  
pp. 02010
Author(s):  
Youdiil Ophinni
Keyword(s):  
Human Genome ◽  
Gene Editing ◽  
Proviral Genome ◽  
Delivery Methods ◽  
Immunodeficiency Virus ◽  
Pathogenic Viruses ◽  
Hiv 1

The innovation of CRISPR-Cas9 has single-handedly revolutionized biotechnology by enabling efficient and specific cutting of DNA. CRISPR-Cas9 approaches are promising not only to targetthe human genome but also DNA of pathogenic viruses, which coincidentally is the canonical function in its bacterial origin. Since 2014, a myriad of studies has proven the efficacy of CRISPR-Cas9 treatment to cleave viral DNA intermediates in vitro. One of the most widely targeted is theproviral genome of human immunodeficiency virus type-1 (HIV-1). The disease burden of HIV-1 is massive—the infection is incurable and has remained a pandemic for over four decades. Integrated HIV-1 provirus inside the human genome causes viral persistence inside latent cellular reservoirs, eluding antiretroviral therapy (ART) and sterilizing cure. Specific targeting anddisruption of HIV-1 proviral genome is necessary to achieve viral clearance, which can be achieved with CRISPR-Cas9. Here, we review the features and up to date evidence of CRISPRCas9 to target the HIV-1 proviral genome and suppress viral replication. We will also discuss potential CRISPR/Cas9 delivery methods in vivo, combination with other gene editing modalities and other therapeutic approaches, to bring gene editing-based HIV-1 cure closer into clinical use.

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