scholarly journals Trans-neuronal transduction of spinal neurons following cortical injection and anterograde axonal transport of a bicistronic AAV1 vector

Gene Therapy ◽  
2015 ◽  
Vol 23 (2) ◽  
pp. 231-236 ◽  
Author(s):  
T H Hutson ◽  
C Kathe ◽  
L D F Moon
Keyword(s):  
Axonal Transport ◽  
Spinal Neurons ◽  
Anterograde Axonal Transport ◽  
Aav1 Vector

The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport

2011 ◽  
Vol 152 (3-4) ◽  
pp. 270-279 ◽  
Author(s):  
S.I. Chowdhury ◽  
M.C.S. Brum ◽  
C. Coats ◽  
A. Doster ◽  
Huiyong Wei ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Envelope Protein ◽  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
Acidic Domain ◽  
Anterograde Axonal Transport ◽  
Bovine Herpesvirus Type 1

scholarly journals Anterograde Axonal Transport in Neuronal Homeostasis and Disease

2020 ◽  
Vol 13 ◽  
Author(s):  
Laurent Guillaud ◽  
Sara Emad El-Agamy ◽  
Miki Otsuki ◽  
Marco Terenzio
Keyword(s):  
Axonal Transport ◽  
Neuronal Homeostasis ◽  
Anterograde Axonal Transport

scholarly journals The Carboxyl Terminus of Tegument Protein pUL21 Contributes to Pseudorabies Virus Neuroinvasion

2019 ◽  
Vol 93 (7) ◽  
Author(s):  
Kai Yan ◽  
Jie Liu ◽  
Xiang Guan ◽  
Yi-Xin Yin ◽  
Hui Peng ◽  
...  
Keyword(s):  
Axonal Transport ◽  
Pseudorabies Virus ◽  
Motor Proteins ◽  
Retrograde Transport ◽  
Cytoplasmic Dynein ◽  
Bimolecular Fluorescence Complementation ◽  
Tegument Protein ◽  
Carboxyl Terminus ◽  
Anterograde Axonal Transport

ABSTRACTFollowing its entry into cells, pseudorabies virus (PRV) utilizes microtubules to deliver its nucleocapsid to the nucleus. Previous studies have shown that PRV VP1/2 is an effector of dynein-mediated capsid transport. However, the mechanism of PRV for recruiting microtubule motor proteins for successful neuroinvasion and neurovirulence is not well understood. Here, we provide evidence that PRV pUL21 is an inner tegument protein. We tested its interaction with the cytoplasmic light chains using a bimolecular fluorescence complementation (BiFC) assay and observed that PRV pUL21 interacts with Roadblock-1. This interaction was confirmed by coimmunoprecipitation (co-IP) assays. We also determined the efficiency of retrograde and anterograde axonal transport of PRV strains in explanted neurons using a microfluidic chamber system and investigated pUL21’s contribution to PRV neuroinvasionin vivo. Further data showed that the carboxyl terminus of pUL21 is essential for its interaction with Roadblock-1, and this domain contributes to PRV retrograde axonal transportin vitroandin vivo. Our findings suggest that the carboxyl terminus of pUL21 contributes to PRV neuroinvasion.IMPORTANCEHerpesviruses are a group of DNA viruses that infect both humans and animals. Alphaherpesviruses are distinguished by their ability to establish latent infection in peripheral neurons. After entering neurons, the herpesvirus capsid interacts with cellular motor proteins and undergoes retrograde transport on axon microtubules. This elaborate process is vital to the herpesvirus lifecycle, but the underlying mechanism remains poorly understood. Here, we determined that pUL21 is an inner tegument protein of pseudorabies virus (PRV) and that it interacts with the cytoplasmic dynein light chain Roadblock-1. We also observed that pUL21 promotes retrograde transport of PRV in neuronal cells. Furthermore, our findings confirm that pUL21 contributes to PRV neuroinvasionin vivo. Importantly, the carboxyl terminus of pUL21 is responsible for interaction with Roadblock-1, and this domain contributes to PRV neuroinvasion. This study offers fresh insights into alphaherpesvirus neuroinvasion and the interaction between virus and host during PRV infection.

Slow anterograde axonal transport of calpain I and II

1990 ◽  
Vol 17 (3) ◽  
pp. 487-494 ◽  
Author(s):  
Elisabeth Nilsson ◽  
Jan-Olof Karlsson
Keyword(s):  
Axonal Transport ◽  
Anterograde Axonal Transport
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