scholarly journals Intranasal administration of plasmid DNA nanoparticles yields successful transfection and expression of a reporter protein in rat brain

Gene Therapy ◽  
2014 ◽  
Vol 21 (5) ◽  
pp. 514-521 ◽  
Author(s):  
B T Harmon ◽  
A E Aly ◽  
L Padegimas ◽  
O Sesenoglu-Laird ◽  
M J Cooper ◽  
...  
Keyword(s):  
Rat Brain ◽  
Plasmid Dna ◽  
Intranasal Administration ◽  
Reporter Protein ◽  
Dna Nanoparticles

Intranasal delivery of hGDNF plasmid DNA nanoparticles results in long-term and widespread transfection of perivascular cells in rat brain

2019 ◽  
Vol 16 ◽  
pp. 20-33 ◽  
Author(s):  
Amirah E-E. Aly ◽  
Brendan Harmon ◽  
Linas Padegimas ◽  
Ozge Sesenoglu-Laird ◽  
Mark J. Cooper ◽  
...  
Keyword(s):  
Rat Brain ◽  
Plasmid Dna ◽  
Intranasal Delivery ◽  
Perivascular Cells ◽  
Dna Nanoparticles

Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy

2014 ◽  
Vol 121 ◽  
pp. 129-140 ◽  
Author(s):  
João Santos ◽  
Fani Sousa ◽  
João Queiroz ◽  
Diana Costa
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Mitochondrial Gene ◽  
Dna Nanoparticles

scholarly journals Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Christian Jreyssaty ◽  
Qin Shi ◽  
Huijie Wang ◽  
Xingping Qiu ◽  
Françoise M. Winnik ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Plasmid Dna ◽  
Intracellular Trafficking ◽  
Cationic Polymers ◽  
Cell Nuclei ◽  
Nonviral Gene Therapy ◽  
Dna Nanoparticles ◽  
Acid Ligand ◽  
Human Epidermoid Carcinoma

Nonviral cationic polymers like chitosan can be combined with DNA to protect it from degradation. The chitosan is a biocompatible, biodegradable, nontoxic, and cheap polycationic polymer with low immunogenicity. The objective of this study was to synthesize and then assess different chitosan-DNA nanoparticles and to select the best ones for selective in vitro transfection in human epidermoid carcinoma (KB) cell lines. It revealed that different combinations of molecular weight, the presence or absence of folic acid ligand, and different plasmid DNA sizes can lead to nanoparticles with various diameters and diverse transfection efficiencies. The intracellular trafficking, nuclear uptake, and localization are also studied by confocal microscopy, which confirmed that DNA was delivered to cell nuclei to be expressed.

Persistence of plasmid DNA and expression in rat brain cells in vivo

1992 ◽  
Vol 115 (3) ◽  
pp. 400-413 ◽  
Author(s):  
Shoushu Jiao ◽  
Gyula Acsadi ◽  
Agnes Jani ◽  
Philip L. Felgner ◽  
Jon A. Wolff
Keyword(s):  
Rat Brain ◽  
Plasmid Dna ◽  
Brain Cells

Development of plasmid DNA nanoparticles for mitochondrial gene therapy

2019 ◽  
pp. 297-327
Author(s):  
Diana Rita Barata Costa ◽  
Fani Pereira de Sousa ◽  
João António de Sampaio Rodrigues Queiroz
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Mitochondrial Gene ◽  
Dna Nanoparticles

Plasmid DNA Nanoparticles for Nonviral Oral Gene Therapy

Nano Letters ◽  
2021 ◽  
Author(s):  
S. M. Shatil Shahriar ◽  
Jeong Man An ◽  
Mohammad Nazmul Hasan ◽  
Sachin S. Surwase ◽  
Yeu-Chun Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Dna Nanoparticles

Preparation of chitosan-plasmid DNA nanoparticles encoding zona pellucida glycoprotein-3? and its expression in mouse

2004 ◽  
Vol 68 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Cai-Jun Sun ◽  
Shan-Pei Pan ◽  
Qi-Xuan Xie ◽  
Luan-Juan Xiao
Keyword(s):  
Zona Pellucida ◽  
Plasmid Dna ◽  
Dna Nanoparticles
Sign in / Sign up

Export Citation Format

Share Document