scholarly journals A novel gene delivery method transduces porcine pancreatic duct epithelial cells

Gene Therapy ◽  
2013 ◽  
Vol 21 (2) ◽  
pp. 123-130 ◽  
Author(s):  
M A Griffin ◽  
M S Restrepo ◽  
M Abu-El-Haija ◽  
T Wallen ◽  
E Buchanan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Delivery Method ◽  
Novel Gene

scholarly journals Magnetic Nanoparticles Mediated-Gene Delivery for Simpler and More Effective Transformation of Pichia pastoris

2021 ◽  
Author(s):  
Seyda Yildiz ◽  
Kubra Solak ◽  
Melek Acar ◽  
Ahmet Mavi ◽  
Yagmur Unver
Keyword(s):  
Magnetic Nanoparticles ◽  
Pichia Pastoris ◽  
Gene Delivery ◽  
Delivery Method ◽  
Exogenous Dna ◽  
Novel Gene ◽  
A Cell

The introduction of exogenous DNA into a cell can be used to produce large quantities of protein. Here, we describe a novel gene delivery method into Pichia pastoris based on...

scholarly journals A human cancer xenograft model utilizing normal pancreatic duct epithelial cells conditionally transformed with defined oncogenes

2014 ◽  
Vol 35 (8) ◽  
pp. 1840-1846 ◽  
Author(s):  
Yuki Inagawa ◽  
Kenji Yamada ◽  
Takashi Yugawa ◽  
Shin-ichi Ohno ◽  
Nobuyoshi Hiraoka ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Human Cancer ◽  
Xenograft Model

The Effect of N-Methyl-N′-nitro-N-nitrosoguanidine (MNNG) on Cultured Dog Pancreatic Duct Epithelial Cells

Pancreas ◽  
1996 ◽  
Vol 12 (2) ◽  
pp. 109-116 ◽  
Author(s):  
Dolphine Oda ◽  
Christopher E. Savard ◽  
Lydia Eng ◽  
Sum P. Lee
Keyword(s):  
Epithelial Cells ◽  
Pancreatic Duct

Bioreducible branched poly(modified nona-arginine) cell-penetrating peptide as a novel gene delivery platform

2017 ◽  
Vol 246 ◽  
pp. 142-154 ◽  
Author(s):  
Jisang Yoo ◽  
DaeYong Lee ◽  
Vipul Gujrati ◽  
N. Sanoj Rejinold ◽  
Kamali Manickavasagam Lekshmi ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cell Penetrating Peptide ◽  
Novel Gene ◽  
Cell Penetrating ◽  
Delivery Platform

scholarly journals The Crosstalk between Nrf2 and TGF-β1 in the Epithelial-Mesenchymal Transition of Pancreatic Duct Epithelial Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132978 ◽  
Author(s):  
Sarah Arfmann-Knübel ◽  
Birte Struck ◽  
Geeske Genrich ◽  
Ole Helm ◽  
Bence Sipos ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Epithelial Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Tgf Β1

scholarly journals Long-Term PEDF Release in Rat Iris and Retinal Epithelial Cells after Sleeping Beauty Transposon-Mediated Gene Delivery

2017 ◽  
Vol 9 ◽  
pp. 1-11 ◽  
Author(s):  
Laura Garcia-Garcia ◽  
Sergio Recalde ◽  
Maria Hernandez ◽  
Jaione Bezunartea ◽  
Juan Roberto Rodriguez-Madoz ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Epithelial Cells ◽  
Sleeping Beauty ◽  
Sleeping Beauty Transposon ◽  
Rat Iris

scholarly journals Regulation of Exocytosis by Protein Kinases and Ca2+ in Pancreatic Duct Epithelial Cells

2000 ◽  
Vol 116 (4) ◽  
pp. 507-520 ◽  
Author(s):  
Duk-Su Koh ◽  
Mark W. Moody ◽  
Toan D. Nguyen ◽  
Bertil Hille
Keyword(s):  
Protein Kinase ◽  
Epithelial Cells ◽  
Pancreatic Duct ◽  
Endocrine Cells ◽  
Cell Types ◽  
Rapid Onset ◽  
Fusion Pore ◽  
Intracellular Camp ◽  
Downstream Effector ◽  
Adrenal Chromaffin

We asked if the mechanisms of exocytosis and its regulation in epithelial cells share features with those in excitable cells. Cultured dog pancreatic duct epithelial cells were loaded with an oxidizable neurotransmitter, dopamine or serotonin, and the subsequent release of these exogenous molecules during exocytosis was detected by carbon-fiber amperometry. Loaded cells displayed spontaneous exocytosis that may represent constitutive membrane transport. The quantal amperometric events induced by fusion of single vesicles had a rapid onset and decay, resembling those in adrenal chromaffin cells and serotonin-secreting leech neurons. Quantal events were frequently preceded by a “foot,” assumed to be leak of transmitters through a transient fusion pore, suggesting that those cell types share a common fusion mechanism. As in neurons and endocrine cells, exocytosis in the epithelial cells could be evoked by elevating cytoplasmic Ca2+ using ionomycin. Unlike in neurons, hyperosmotic solutions decreased exocytosis in the epithelial cells, and giant amperometric events composed of many concurrent quantal events were observed occasionally. Agents known to increase intracellular cAMP in the cells, such as forskolin, epinephrine, vasoactive intestinal peptide, or 8-Br-cAMP, increased the rate of exocytosis. The forskolin effect was inhibited by the Rp-isomer of cAMPS, a specific antagonist of protein kinase A, whereas the Sp-isomer, a specific agonist of PKA, evoked exocytosis. Thus, PKA is a downstream effector of cAMP. Finally, activation of protein kinase C by phorbol-12-myristate-13-acetate also increased exocytosis. The PMA effect was not mimicked by the inactive analogue, 4α-phorbol-12,13-didecanoate, and it was blocked by the PKC antagonist, bisindolylmaleimide I. Elevation of intracellular Ca2+ was not needed for the actions of forskolin or PMA. In summary, exocytosis in epithelial cells can be stimulated directly by Ca2+, PKA, or PKC, and is mediated by physical mechanisms similar to those in neurons and endocrine cells.

scholarly journals Pattern of Ca2+increase determines the type of secretory mechanism activated in dog pancreatic duct epithelial cells

2006 ◽  
Vol 576 (1) ◽  
pp. 163-178 ◽  
Author(s):  
Seung-Ryoung Jung ◽  
Kyungjin Kim ◽  
Bertil Hille ◽  
Toan D. Nguyen ◽  
Duk-Su Koh
Keyword(s):  
Epithelial Cells ◽  
Pancreatic Duct

Bovine pancreatic duct cells express cAMP- and Ca(2+)-activated apical membrane Cl- conductances

1997 ◽  
Vol 273 (1) ◽  
pp. G204-G216 ◽  
Author(s):  
L. al-Nakkash ◽  
C. U. Cotton
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Pancreatic Duct ◽  
Apical Membrane ◽  
Apical Cell ◽  
Primary Cultures ◽  
Apical Cell Membrane ◽  
Anion Secretion ◽  
Duct Epithelium ◽  
Cl Permeability

Secretion of salt and water by the epithelial cells that line pancreatic ducts depends on activation of apical membrane Cl- conductance. In the present study, we characterized two types of Cl- conductances present in the apical cell membrane of bovine pancreatic duct epithelial cells. Primary cultures of bovine main pancreatic duct epithelium and an immortalized cell line (BPD1) derived from primary cultures were used. Elevation of intracellular adenosine 3',5'-cyclic monophosphate (cAMP) or Ca2+ in intact monolayers of duct epithelium induced sustained anion secretion. Agonist-induced changes in plasma membrane Cl- permeability were accessed by 36 Cl- efflux, whole cell current recording, and measurements of transepithelial Cl- current across permeabilized epithelial monolayers. Elevation of intracellular cAMP elicited a sustained increase in Cl- permeability, whereas elevation of intracellular Ca2+ induced only a transient increase in Cl- permeability. Ca(2+)- but not cAMP-induced increases in Cl- permeability were abolished by preincubation of cells with the Ca2+ buffer 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetra(acetoxymethyl) ester (BAPTA-AM). N-phenylanthranilic acid (DPC; 1 mM) and glibenclamide (100 microM), but not 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; 500 microM), inhibited the cAMP-induced increase in Cl- permeability. In contrast, DPC and DIDS, but not glibenclamide, inhibited the Ca(2+)-induced increase in Cl- permeability. We conclude from these experiments that bovine pancreatic duct epithelial cells express at least two types of Cl- channels, cAMP and Ca2+ activated, in the apical cell membrane. Because the Ca(2+)-activated increase in Cl- permeability is transient, the extent to which this pathway contributes to sustained anion secretion by the ductal epithelium remains to be determined.

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