scholarly journals TAP-deficient human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen-presenting cells

Gene Therapy ◽  
2012 ◽  
Vol 20 (5) ◽  
pp. 504-513 ◽  
Author(s):  
M Haruta ◽  
Y Tomita ◽  
A Yuno ◽  
K Matsumura ◽  
T Ikeda ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Cell Lines ◽  
Myeloid Cell ◽  
Antigen Presenting Cells ◽  
Ips Cell ◽  
Cell Source ◽  
Antigen Presenting

540 Transcriptionally defined immune landscape in human gliomas

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A576-A576
Author(s):  
Pravesh Gupta ◽  
Minghao Dang ◽  
Krishna Bojja ◽  
Huma Shehwana ◽  
Tuan Tran ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Single Cell ◽  
Rna Sequencing ◽  
Immune Cell ◽  
Myeloid Cell ◽  
Wild Type ◽  
Cell Clusters ◽  
Md Anderson ◽  
Antigen Presenting

BackgroundBrain immunity is largely myeloid cell dominated rather than lymphoid cells in healthy and diseased state including malignancies of glial origins called as gliomas. Despite this skewed myeloid centric immune contexture, immune checkpoint and T cell based therapeutic modalities are generalizably pursued in gliomas ignoring the following facts i) T cells are sparse in tumor brain ii) glioma patients are lymphopenic iii) gliomas harbor abundant and highly complex myeloid cell repertoire. We recognized these paradoxes pertaining to fundamental understanding of constituent immune cells and their functional states in the tumor immune microenvironment (TIME) of gliomas, which remains elusive beyond a priori cell types and/or states.MethodsTo dissect the TIME in gliomas, we performed single-cell RNA-sequencing on ~123,000 tumor-derived sorted CD45+ leukocytes from fifteen genomically classified patients comprising IDH-mutant primary (IMP; n=4), IDH-mutant recurrent (IMR; n=4), IDH-wild type primary (IWP; n=3), or IDH-wild type recurrent (IWR; n=4) gliomas (hereafter referred as glioma subtypes) and two non-glioma brains (NGBs) as controls.ResultsUnsupervised clustering analyses delineated predominant 34-myeloid cell clusters (~75%) over 28-lymphoid cell clusters (~25%) reflecting enormous heterogeneity within and across glioma subtypes. The glioma immune diversity spanned functionally imprinted phagocytic, antigen-presenting, hypoxia, angiogenesis and, tumoricidal myeloid to classical cytotoxic lymphoid subpopulations. Specifically, IDH-mutant gliomas were predominantly enriched for brain-resident microglial subpopulations in contrast to enriched bone barrow-derived infiltrates in IDH-wild type especially in a recurrent setting. Microglia attrition in IWP and IWR gliomas were concomitant with invading monocyte-derived cells with semblance to dendritic cell and macrophage like transcriptomic features. Additionally, microglial functional diversification was noted with disease severity and mostly converged to inflammatory states in IWR gliomas. Beyond dendritic cells, multiple antigen-presenting cellular states expanded with glioma severity especially in IWP and IWR gliomas. Furthermore, we noted differential microglia and dendritic cell inherent antigen presentation axis viz, osteopontin, and classical HLAs in IDH subtypes and, glioma-wide non-PD1 checkpoints associations in T cells like Galectin9 and Tim-3. As a general utility, our immune cell deconvolution approach with single-cell-matched bulk RNA sequencing data faithfully resolved 58-cell states which provides glioma specific immune reference for digital cytometry application to genomics datasets.ConclusionsAltogether, we identified prognosticator immune cell-signatures from TCGA cohorts as one of many potential immune responsiveness applications of the curated signatures for basic and translational immune-genomics efforts. Thus, we not only provide an unprecedented insight of glioma TIME but also present an immune data resource that can be exploited for immunotherapy applications.Ethics ApprovalThe brain tumor/tissue samples were collected as per MD Anderson internal review board (IRB)-approved protocol numbers LAB03-0687 and, LAB04-0001. One non-tumor brain tissue sample was collected from patient undergoing neurosurgery for epilepsy as per Baylor College of Medicine IRB-approved protocol number H-13798. All experiments were compliant with the review board of MD Anderson Cancer Center, USA.ConsentWritten informed consent was obtained from the patient for publication of this abstract and any accompanying images. A copy of the written consent is available for review by the Editor of this journal

The accumulation of [35S]methimazole by monocytes and macrophages

1984 ◽  
Vol 107 (3) ◽  
pp. 366-370 ◽  
Author(s):  
A. P. Weetman ◽  
Catherine Gunn ◽  
R. Hall ◽  
A. M. McGregor
Keyword(s):  
Cell Lines ◽  
Antithyroid Drug ◽  
Antigen Presenting Cells ◽  
Leukaemic Cell ◽  
Graves Disease ◽  
Temperature Dependent ◽  
Culture Techniques ◽  
Monocytes And Macrophages ◽  
Significant Temperature ◽  
Antigen Presenting

Abstract. We have used an automatic cell harvester and micro culture techniques to examine the accumulation of [35S]methimazole by monocytes, macrophages and lymphocytes. Significant temperature-dependent accumulation of the drug was found in resting monocytes and macrophages; this was increased up to 4-fold by phagocytosis. Lymphocytes accumulated little or no drug and myeloma and leukaemic cell lines accumulated none. These results show that two interrelated cells with endogenous peroxidatic activity take up the antithyroid drug methimazole providing further support for the concept that immunosuppression by this drug in Graves' disease is mediated via an action on antigen-presenting cells.

scholarly journals Spleen as a Site for Hematopoiesis of a Distinct Antigen Presenting Cell Type

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Helen C. O'Neill ◽  
Kristin L. Griffiths ◽  
Pravin Periasamy ◽  
Rebecca A. Hinton ◽  
Ying-Ying Hey ◽  
...  
Keyword(s):  
Specific Antigen ◽  
Myeloid Cell ◽  
Antigen Presenting Cells ◽  
Cell Types ◽  
The Novel ◽  
Cell Type ◽  
Hematopoietic Stem ◽  
Secondary Tissue ◽  
A Site ◽  
Antigen Presenting

While spleen and other secondary tissue sites contribute to hematopoiesis, the nature of cells produced and the environment under which this happens are not fully defined. Evidence is reviewed here for hematopoiesis occurring in the spleen microenvironment leading to the production of tissue-specific antigen presenting cells. The novel dendritic-like cell identified in spleen is phenotypically and functionally distinct from other described antigen presenting cells. In order to identify these cells as distinct, it has been necessary to show that their lineage origin and progenitors differ from that of other known dendritic and myeloid cell types. The spleen therefore represents a distinct microenvironment for hematopoiesis of a novel myeloid cell arising from self-renewing hematopoietic stem cells (HSC) or progenitors endogenous to spleen.

Immunotherapy against Metastatic Melanoma with Human iPS Cell–Derived Myeloid Cell Lines Producing Type I Interferons

2015 ◽  
Vol 4 (3) ◽  
pp. 248-258 ◽  
Author(s):  
Azusa Miyashita ◽  
Satoshi Fukushima ◽  
Satoshi Nakahara ◽  
Yosuke Kubo ◽  
Aki Tokuzumi ◽  
...  
Keyword(s):  
Metastatic Melanoma ◽  
Cell Lines ◽  
Myeloid Cell ◽  
Type I Interferons ◽  
Type I ◽  
Ips Cell

Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors

2009 ◽  
Vol 154 (7) ◽  
pp. 1129-1138 ◽  
Author(s):  
John C. Martyn ◽  
Anthony J. Cardin ◽  
Bruce D. Wines ◽  
Angela Cendron ◽  
Shuo Li ◽  
...  
Keyword(s):  
Cell Lines ◽  
Surface Display ◽  
Fc Receptors ◽  
Antigen Presenting Cells ◽  
Antigen Presenting

Generation of antigen-specific cytotoxic T lymphocytes using a leukemic plasmacytoid dendritic cell line as antigen presenting cells

2011 ◽  
Vol 35 (6) ◽  
pp. 793-799 ◽  
Author(s):  
Akie Yamahira ◽  
Miwako Narita ◽  
Takeshi Nakamura ◽  
Norihiro Watanabe ◽  
Masami Kaji ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
T Lymphocytes ◽  
Cell Line ◽  
Cytotoxic T Lymphocytes ◽  
Plasmacytoid Dendritic Cell ◽  
Antigen Presenting Cells ◽  
Antigen Presenting
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