scholarly journals Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair

Gene Therapy ◽  
2010 ◽  
Vol 17 (9) ◽  
pp. 1175-1180 ◽  
Author(s):  
M L Hirsch ◽  
L Green ◽  
M H Porteus ◽  
R J Samulski
Keyword(s):  
Gene Targeting ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Break Repair

scholarly journals Incorporation of Large Heterologies Into Heteroduplex DNA During Double-Strand-Break Repair in Mouse Cells

Genetics ◽  
2002 ◽  
Vol 162 (2) ◽  
pp. 977-985
Author(s):  
Steven J Raynard ◽  
Mark D Baker
Keyword(s):  
Dna Replication ◽  
Gene Targeting ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Hybridoma Cells ◽  
Heteroduplex Dna ◽  
Mouse Cells ◽  
Break Repair ◽  
Efficient Recovery

AbstractIn this study, the formation and repair of large (>1 kb) insertion/deletion (I/D) heterologies during double-strand-break repair (DSBR) was investigated using a gene-targeting assay that permits efficient recovery of sequence insertion events at the haploid chromosomal immunoglobulin (Ig) μ-locus in mouse hybridoma cells. The results revealed that (i) large I/D heterologies were generated on one or both sides of the DSB and, in some cases, formed symmetrically in both homology regions; (ii) large I/D heterologies did not negatively affect the gene targeting frequency; and (iii) prior to DNA replication, the large I/D heterologies were rectified.

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