scholarly journals Artemis splice defects cause atypical SCID and can be restored in vitro by an antisense oligonucleotide

2011 ◽  
Vol 12 (6) ◽  
pp. 434-444 ◽  
Author(s):  
H IJspeert ◽  
A C Lankester ◽  
J M van den Berg ◽  
W Wiegant ◽  
M C van Zelm ◽  
...  
Keyword(s):  
Antisense Oligonucleotide ◽  
Atypical Scid

scholarly journals Ethylcellulose nanoparticles as a new “in vitro” transfection tool for antisense oligonucleotide delivery

2020 ◽  
Vol 229 ◽  
pp. 115451 ◽  
Author(s):  
S. Leitner ◽  
S. Grijalvo ◽  
C. Solans ◽  
R. Eritja ◽  
M.J. García-Celma ◽  
...  
Keyword(s):  
Antisense Oligonucleotide ◽  
Oligonucleotide Delivery ◽  
In Vitro Transfection

scholarly journals Muscle-specific microRNA miR-206 promotes muscle differentiation

2006 ◽  
Vol 174 (5) ◽  
pp. 677-687 ◽  
Author(s):  
Hak Kyun Kim ◽  
Yong Sun Lee ◽  
Umasundari Sivaprasad ◽  
Ankit Malhotra ◽  
Anindya Dutta
Keyword(s):  
Antisense Oligonucleotide ◽  
Small Interfering Rna ◽  
Degradation Process ◽  
Microrna Target ◽  
C2c12 Myoblasts ◽  
Target Sites ◽  
The Many ◽  
Interfering Rna ◽  
In Vitro Transfection

Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase α and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA–directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.

scholarly journals Novel Disulfide-Bridged Bioresponsive Antisense Oligonucleotide Induces Efficient Splice Modulation in Muscle Myotubes in Vitro

ACS Omega ◽  
2020 ◽  
Vol 5 (29) ◽  
pp. 18035-18039
Author(s):  
Bao T. Le ◽  
Tamer R. Kosbar ◽  
Rakesh N. Veedu
Keyword(s):  
Antisense Oligonucleotide

The uptake of a fluorescently labelled antisense oligonucleotide in vitro and in vivo

2005 ◽  
Vol 147 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Emma S.J. Robinson ◽  
David J. Nutt ◽  
Helen C. Jackson ◽  
Alan L. Hudson
Keyword(s):  
Antisense Oligonucleotide

scholarly journals Magnetofection—A highly efficient tool for antisense oligonucleotide delivery in vitro and in vivo

2003 ◽  
Vol 7 (5) ◽  
pp. 700-710 ◽  
Author(s):  
Florian Krötz ◽  
Cor de Wit ◽  
Hae-Young Sohn ◽  
Stefan Zahler ◽  
Torsten Gloe ◽  
...  
Keyword(s):  
Antisense Oligonucleotide ◽  
Efficient Tool ◽  
Highly Efficient ◽  
Oligonucleotide Delivery

An anionic long-circulating liposome that improves radioiodinated antisense oligonucleotide delivery in vitro and in vivo

2011 ◽  
Vol 31 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Jiawei Xie ◽  
Chao Ma ◽  
Jizheng Lin ◽  
Guoming Wang ◽  
Anren Kuang ◽  
...  
Keyword(s):  
Antisense Oligonucleotide ◽  
Oligonucleotide Delivery
Sign in / Sign up

Export Citation Format

Share Document