Bacterial agglutination: Stick-to-nonstick switch

2010 ◽  
Author(s):  
Heather Buschman
Keyword(s):  
Bacterial Agglutination

scholarly journals OBSERVATIONS ON THE AGGLUTINATION OF POLYSACCHARIDE-TREATED ERYTHROCYTES BY TULAREMIA ANTISERA

1950 ◽  
Vol 91 (6) ◽  
pp. 561-566 ◽  
Author(s):  
Mary M. Alexander ◽  
George G. Wright ◽  
Agnes C. Baldwin
Keyword(s):  
Agglutination Titer ◽  
Hemagglutination Titer ◽  
Bacterial Agglutination

Erythrocytes treated with polysaccharide from Past. tularensis are specifically agglutinated by the sera of persons recovered from tularemia or vaccinated against it, and by the sera of animals immunized with living cultures. The serum hemagglutination titer is correlated with the amount of antibody as measured by the quantitative precipitin method with polysaccharide, but not with the bacterial agglutination titer. The meaning of the results is discussed.

scholarly journals Dimerisation and structural integrity of Heparin Binding Hemagglutinin A from Mycobacterium tuberculosis : Implications for bacterial agglutination

FEBS Letters ◽  
2010 ◽  
Vol 584 (6) ◽  
pp. 1091-1096 ◽  
Author(s):  
Carla Esposito ◽  
Paola Carullo ◽  
Emilia Pedone ◽  
Giuseppe Graziano ◽  
Pompea Del Vecchio ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Structural Integrity ◽  
Heparin Binding ◽  
Bacterial Agglutination

scholarly journals BACTERIAL AGGLUTINATION BY A LECTIN FROM THE LEAVES OF THE MEDICINAL PLANT, PIMENTA DIOICA (L.) MERR

2018 ◽  
Vol 10 (4) ◽  
pp. 35 ◽  
Author(s):  
Surya P H ◽  
Elyas K K ◽  
Deepti Madayi
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plant ◽  
Biochemical Characterization ◽  
Exchange Chromatography ◽  
Bacterial Typing ◽  
Gram Negative ◽  
Sds Page ◽  
Bacterial Filters ◽  
Pimenta Dioica ◽  
Bacterial Agglutination

Objective: The current investigation involves the purification, characterization of the lectin from the leaves of Pimenta dioica (L.) Merr. (Myrtaceae) a medicinal plant, and its application in bacterial typing.Methods: A lectin was purified from the leaves by cation exchange chromatography. SDS PAGE revealed the molecular weight of the purified lectin. Biochemical characterization was carried out by performing various tests. Hemagglutination inhibition was conducted to detect the sugar specificity. Additionally, bacterial agglutination was performed to predict whether the purified lectin was able to agglutinate the bacterial strains.Results: SDS PAGE analysis revealed the lectin to be a tetramer in the range of 43-66 kDa. The purified lectin agglutinated human, avian, and mouse erythrocytes, and was inhibited by 125 mmol of mannose and xylose. The lectin was stable at 0-60 ° C for 30 min and was unaffected by either 2-Mercaptoethanol (2-ME) or Dithiothreitol (DTT) (50-250µM). A pH of 6.0–8.0 was found optimum for its activity and was nearly independent of metal ions. The purified lectin contained about 20% carbohydrate as estimated by Anthrone method. Purified lectin agglutinated the Gram-negative Escherichia coli and Proteus vulgaris.Conclusion: The isolated lectin was found to possess significant hemagglutinating activity. Due to its ability to agglutinate Gram negative bacteria such as Escherichia coli and Proteus vulgaris, it could be used for bacterial typing and for the design of bacterial filters.

scholarly journals Characterization of a C-Type Lectin Domain-Containing Protein with Antibacterial Activity from Pacific Abalone (Haliotis discus hannai)

2022 ◽  
Vol 23 (2) ◽  
pp. 698
Author(s):  
Mi-Jin Choi ◽  
Yeo Reum Kim ◽  
Nam Gyu Park ◽  
Cheorl-Ho Kim ◽  
Young Dae Oh ◽  
...  
Keyword(s):  
Defense Mechanism ◽  
Developmental Stages ◽  
Haliotis Discus Hannai ◽  
Gene Encoding ◽  
Pacific Abalone ◽  
Reading Frame ◽  
Lectin Domain ◽  
Haliotis Discus ◽  
Abalone Haliotis Discus ◽  
Bacterial Agglutination

Genes that influence the growth of Pacific abalone (Haliotis discus hannai) may improve the productivity of the aquaculture industry. Previous research demonstrated that the differential expression of a gene encoding a C-type lectin domain-containing protein (CTLD) was associated with a faster growth in Pacific abalone. We analyzed this gene and identified an open reading frame that consisted of 145 amino acids. The sequence showed a significant homology to other genes that encode CTLDs in the genus Haliotis. Expression profiling analysis at different developmental stages and from various tissues showed that the gene was first expressed at approximately 50 days after fertilization (shell length of 2.47 ± 0.13 mm). In adult Pacific abalone, the gene was strongly expressed in the epipodium, gill, and mantle. Recombinant Pacific abalone CTLD purified from Escherichia coli exhibited antimicrobial activity against several Gram-positive bacteria (Bacillus subtilis, Streptococcus iniae, and Lactococcus garvieae) and Gram-negative bacteria (Vibrio alginolyticus and Vibrio harveyi). We also performed bacterial agglutination assays in the presence of Ca2+, as well as bacterial binding assays in the presence of the detergent dodecyl maltoside. Incubation with E. coli and B. subtilis cells suggested that the CTLD stimulated Ca2+-dependent bacterial agglutination. Our results suggest that this novel Pacific abalone CTLD is important for the pathogen recognition in the gastropod host defense mechanism.

Bacterial Agglutination and Polyacrylamide Gel Electrophoresis for Typing Clostridium difficile

1986 ◽  
Vol 153 (2) ◽  
pp. 267-271 ◽  
Author(s):  
M. E. Mulligan ◽  
S. Halebian ◽  
R. Y. Y. Kwok ◽  
W. C. Cheng ◽  
S. M. Finegold ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Bacterial Agglutination

scholarly journals Cover Picture: Tunable Bacterial Agglutination and Motility Inhibition by Self-Assembled Glyco-Nanoribbons (Chem. Asian J. 11/2007)

2007 ◽  
Vol 2 (11) ◽  
pp. 1331-1331
Author(s):  
Yong-beom Lim ◽  
Somi Park ◽  
Eunji Lee ◽  
Ja-Hyoung Ryu ◽  
You-Rim Yoon ◽  
...  
Keyword(s):  
Self Assembled ◽  
Bacterial Agglutination ◽  
Motility Inhibition

Letters to the Editor

PEDIATRICS ◽  
1966 ◽  
Vol 38 (3) ◽  
pp. 522-523
Author(s):  
SAMUEL P. GOTOFF ◽  
WILLIAM D. COCHRAN
Keyword(s):  
Molecular Level ◽  
Protein Antigens ◽  
Letters To The Editor ◽  
Antibody Synthesis ◽  
Immunological Tests ◽  
Bacterial Agglutination ◽  
Primary Interaction

Doctors Minden and Rothberg properly point out that certain immunological tests are more efficient in detecting γM than γG antibody, and we concur that, when one is examining antibody synthesis on a molecular level, tests which detect the primary interaction between antigen and antibody are more desirable. Until very recently the latter tests have been restricted to highly purified protein antigens, and only tests which detect secondary manifestations such as bacterial agglutination, hemagglutination, bacteriolysis, opsonization, and mouse protection tests have been used to study antibodies to the complex antigens of Gramnegative bacteria.

scholarly journals Surfactant Protein D Inhibition of Human Macrophage Uptake ofMycobacterium tuberculosisIs Independent of Bacterial Agglutination

2002 ◽  
Vol 168 (3) ◽  
pp. 1309-1314 ◽  
Author(s):  
J. Scott Ferguson ◽  
Dennis R. Voelker ◽  
Jennifer A. Ufnar ◽  
Amanda J. Dawson ◽  
Larry S. Schlesinger
Keyword(s):  
Surfactant Protein ◽  
Surfactant Protein D ◽  
Human Macrophage ◽  
Macrophage Uptake ◽  
Bacterial Agglutination
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