Alzheimer disease and protein turnover: A GlcNAc trick

Hydroxynonenal-Generated Crosslinking Fluorophore and Mitochondria-derived Lipoic Acid Accumulation in Alzheimer Disease Reveal a Dichotomy of Protein Turnover

Microscopy and Microanalysis ◽

10.1017/s1431927610059350 ◽

2010 ◽

Vol 16 (S2) ◽

pp. 1014-1015

Author(s):

RJ Castellani ◽

P Moreira ◽

G Aliev ◽

JC Shenk ◽

SL Siedlak ◽

...

Keyword(s):

Alzheimer Disease ◽

Lipoic Acid ◽

Protein Turnover ◽

Acid Accumulation

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

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Hydroxynonenal-generated crosslinking fluorophore accumulation in Alzheimer disease reveals a dichotomy of protein turnover

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2011.11.004 ◽

2012 ◽

Vol 52 (3) ◽

pp. 699-704 ◽

Cited By ~ 23

Author(s):

Xiongwei Zhu ◽

Rudy J. Castellani ◽

Paula I. Moreira ◽

Gjumrakch Aliev ◽

Justin C. Shenk ◽

...

Keyword(s):

Alzheimer Disease ◽

Protein Turnover

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Structural and Chemical Studies of Pathological Fibers in Human Neurofibrillary Degeneration

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012031x ◽

1985 ◽

Vol 43 ◽

pp. 726-729

Author(s):

K.S. Kosik ◽

L.K. Duffy ◽

S. Bakalis ◽

C. Abraham ◽

D.J. Selkoe

Keyword(s):

Monoclonal Antibodies ◽

Alzheimer Disease ◽

Human Brain ◽

Neurofibrillary Tangles ◽

Morphological Analysis ◽

High Specificity ◽

Cytoskeletal Proteins ◽

Neuritic Plaque ◽

Protein Chemistry ◽

Chemical Studies

The major structural lesions of the human brain during aging and in Alzheimer disease (AD) are the neurofibrillary tangles (NFT) and the senile (neuritic) plaque. Although these fibrous alterations have been recognized by light microscopists for almost a century, detailed biochemical and morphological analysis of the lesions has been undertaken only recently. Because the intraneuronal deposits in the NFT and the plaque neurites and the extraneuronal amyloid cores of the plaques have a filamentous ultrastructure, the neuronal cytoskeleton has played a prominent role in most pathogenetic hypotheses.The approach of our laboratory toward elucidating the origin of plaques and tangles in AD has been two-fold: the use of analytical protein chemistry to purify and then characterize the pathological fibers comprising the tangles and plaques, and the use of certain monoclonal antibodies to neuronal cytoskeletal proteins that, despite high specificity, cross-react with NFT and thus implicate epitopes of these proteins as constituents of the tangles.

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