scholarly journals Local complement activation in aqueous humor in patients with age-related macular degeneration

Eye ◽  
2017 ◽  
Vol 31 (5) ◽  
pp. 810-813 ◽  
Author(s):  
T Schick ◽  
M Steinhauer ◽  
A Aslanidis ◽  
L Altay ◽  
M Karlstetter ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Aqueous Humor ◽  
Complement Activation ◽  
Age Related Macular Degeneration ◽  
Age Related

scholarly journals Local complement activation in aqueous humor in patients with age-related macular degeneration

2017 ◽  
Vol 89 ◽  
pp. 163 ◽  
Author(s):  
Michael Kirschfink ◽  
Tina Schick ◽  
Marlin Steinhauer ◽  
Alexander Aslanidis ◽  
Lebriz Altay ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Aqueous Humor ◽  
Complement Activation ◽  
Age Related Macular Degeneration ◽  
Age Related

scholarly journals Association of imaging biomarkers and local activation of complement in aqueous humor of patients with early forms of age-related macular degeneration

2020 ◽  
Author(s):  
Vasilena Sitnilska ◽  
Philip Enders ◽  
Claus Cursiefen ◽  
Sascha Fauser ◽  
Lebriz Altay
Keyword(s):  
Macular Degeneration ◽  
Aqueous Humor ◽  
Complement Activation ◽  
Rank Correlation ◽  
Age Related Macular Degeneration ◽  
Imaging Biomarkers ◽  
Complement Factor ◽  
Complement Protein ◽  
Complement Factors ◽  
Age Related

Abstract Purpose To investigate a possible correlation between established imaging biomarkers for age-related macular degeneration and local complement system activation, measured in aqueous humor (AH) of patients with early stages of age-related macular degeneration (AMD) and controls. Methods This analysis included prospectively acquired AH samples of 106 eyes (35 with early/intermediate AMD, 71 controls). The levels of complement protein 3 (C3), 4 (C4), 5 (C5); activation products of complement factor 3a (C3a) and Ba, C3b/iC3b; complement factors B, D, H, I (CFB, CFD, CFH, CFI); and total protein concentration were analyzed. Quantitative levels of complement factors were correlated to the presence of reticular pseudodrusen (RPD), the presence of hyperreflective foci (HRF), and total drusen volume (DV) graded on imaging by spectral-domain optical coherence tomography and using Spearman’s rank correlation test. Results DV correlated with C3b/iC3b (r = 0.285; P = 0.034), C3a (r = 0.200; P = 0.047), Ba (r = 0.262; P = 0.009), and C5 (r = 430; P = 0.005), and showed a tendency towards correlation with C3a (r = 0.198; P = 0.057). HRF correlated significantly with C5 (r = 0.388; P = 0.011) and RPD showed a tendency towards correlation with CFB (r = 0.196; P = 0.050). Conclusion In patients with early AMD, HRF and drusen parameters but not RPD show low to fair levels of correlation with local complement activation in patients’ AH. Better understanding of complement activation could provide some insights into the pathogenesis of AMD. Imaging biomarkers could be useful to identify suitable patients for future clinical trials with complement-modulating therapies.

scholarly journals LRG1 Expression Is Elevated in the Eyes of Patients with Neovascular Age-Related Macular Degeneration

2021 ◽  
Vol 22 (16) ◽  
pp. 8879
Author(s):  
Lucia Mundo ◽  
Gian Marco Tosi ◽  
Stefano Lazzi ◽  
Grazia Pertile ◽  
Barbara Parolini ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Aqueous Humor ◽  
Smooth Muscle Actin ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Average Concentration ◽  
Age Related Macular Degeneration ◽  
Surrounding Tissue ◽  
Anti Vegf ◽  
Age Related

Leucine-rich a-2-glycoprotein 1 (LRG1) is a candidate therapeutic target for treating the neovascular form of age-related macular degeneration (nvAMD). In this study we examined the expression of LRG1 in eyes of nvAMD patients. Choroidal neovascular membranes (CNVMs) from patients who underwent submacular surgery for retinal pigment epithelium–choroid graft transplantation were collected from 5 nvAMD patients without any prior intravitreal anti-VEGF injection, and from six patients who received intravitreal anti-VEGF injections before surgery. As controls free of nvAMD, retina sections were obtained from the eyes resected from a patient with lacrimal sac tumor and from a patient with neuroblastoma. CNVMs were immunostained for CD34, LRG1, and α-smooth muscle actin (α-SMA). Aqueous humor samples were collected from 58 untreated-naïve nvAMD patients prior to the intravitreal injection of anti-VEGF and 51 age-matched cataract control patients, and LRG1 concentration was measured by ELISA. The level of LRG1 immunostaining is frequently high in both the endothelial cells of the blood vessels, and myofibroblasts in the surrounding tissue of CNVMs of treatment-naïve nvAMD patients. Furthermore, the average concentration of LRG1 was significantly higher in the aqueous humor of nvAMD patients than in controls. These observations provide a strong experimental basis and scientific rationale for the progression of a therapeutic anti-LRG1 monoclonal antibody into clinical trials with patients with nvAMD.

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