scholarly journals Inhibition of γ ray-induced apoptosis by stimulatory heterotrimeric GTP binding protein involves Bcl-xL down-regulation in SH-SY5Y human neuroblastoma cells

2007 ◽  
Vol 39 (5) ◽  
pp. 583-593 ◽  
Author(s):  
So-Young Kim ◽  
Miran Seo ◽  
Jung-Min Oh ◽  
Eun-Ah Cho ◽  
Yong-Sung Juhnn
Keyword(s):  
Binding Protein ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Gtp Binding Protein ◽  
Down Regulation ◽  
Human Neuroblastoma ◽  
Gtp Binding ◽  
Γ Ray ◽  
Induced Apoptosis

Expression and Down-Regulation by Retinoic Acid of IGF Binding Protein-2 and -4 in Medium from Human Neuroblastoma Cells

1994 ◽  
Vol 6 (4) ◽  
pp. 409-413 ◽  
Author(s):  
Sergio Bernardini ◽  
Stefano Cianfarani ◽  
Anna Spagnoli ◽  
Margherita Annicchiarico-Petruzzelli ◽  
Gerry Melino ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Binding Protein ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Down Regulation ◽  
Human Neuroblastoma ◽  
Igf Binding ◽  
Igf Binding Protein

scholarly journals Stimulatory Heterotrimeric GTP-binding Protein Inhibits Hydrogen Peroxide-induced Apoptosis by Repressing BAK Induction in SH-SY5Y Human Neuroblastoma Cells

2007 ◽  
Vol 283 (3) ◽  
pp. 1350-1361 ◽  
Author(s):  
So-Young Kim ◽  
MiRan Seo ◽  
Yeni Kim ◽  
Yun-Il Lee ◽  
Jung-Min Oh ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Neuroblastoma Cells ◽  
Camp Signaling ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Gtp Binding ◽  
Camp Signaling Pathway ◽  
Induced Apoptosis

Heterotrimeric stimulatory GTP-binding protein (Gs) stimulates adenylate cyclases to activate the cAMP signaling pathway. Although the cAMP pathway has been reported to be involved in apoptosis, the role of the Gs-cAMP signaling pathway during reactive oxygen species (ROS)-mediated apoptosis, which is involved in the resistance of cancer cells to chemotherapy and radiation, is not clearly understood. Thus, in this study we aimed to investigate the role of the α subunit of Gs (Gαs) in the ROS-induced apoptosis of cancer cells. The stable expression of constitutively active Gαs (GαsQL) inhibited the hydrogen peroxide-induced apoptosis of SH-SY5Y human neuroblastoma cells and reduced the hydrogen peroxide-induced increase in Bak and the decrease in Bcl-xL protein expression. Exogenous Bak expression abolished these inhibitory effects of GαsQL, but Bak small interfering RNA decreased hydrogen peroxide-induced apoptosis. Gαs repressed hydrogen peroxide-induced Bak expression by inhibiting the transcription of Bak mRNA, which resulted from the inhibition of the hydrogen peroxide-induced activation of transcription factors such as AP1, NF-κB, and NFAT. Moreover, Gαs also inhibited the hydrogen peroxide-induced binding of AP1, NF-κB, and NFAT to the Bak promoter. Furthermore, hydrogen peroxide-induced apoptosis was reduced by treating cells with prostaglandin E2, which activates Gαs, but this was augmented by CCPA, which activates Gαi causing a decrease in cAMP levels. From the results, we conclude that Gαs protects neuroblastoma cells from hydrogen peroxide-induced apoptosis by repressing Bak induction, which is mediated by the inhibition of the hydrogen peroxide-induced activations of AP1, NF-κB, and NFAT through cAMP-PKA-CREB signaling system.

Bax-activation during lidocaine-induced apoptosis in human neuroblastoma cells

2007 ◽  
Vol 32 (Suppl. 1) ◽  
pp. 186
Author(s):  
M. F. Stevens ◽  
S. Braun ◽  
H. Hermanns ◽  
P. Lipfert ◽  
F. Essmann ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Induced Apoptosis

Morroniside Prevents Peroxide-induced Apoptosis by Induction of Endogenous Glutathione in Human Neuroblastoma Cells

2007 ◽  
Vol 28 (2) ◽  
pp. 293-305 ◽  
Author(s):  
Wen Wang ◽  
Wenting Huang ◽  
Lin Li ◽  
Houxi Ai ◽  
Fangling Sun ◽  
...  
Keyword(s):  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Induced Apoptosis

Fraxetin prevents rotenone-induced apoptosis by induction of endogenous glutathione in human neuroblastoma cells

2005 ◽  
Vol 53 (1) ◽  
pp. 48-56 ◽  
Author(s):  
María Isabel Sánchez-Reus ◽  
Irene Iglesias Peinado ◽  
María Francisca Molina-Jiménez ◽  
Juana Benedí
Keyword(s):  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Induced Apoptosis
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