scholarly journals The proteins of synaptic vesicle membranes are affected during ageing of rat brain

2001 ◽  
Vol 33 (4) ◽  
pp. 220-225 ◽  
Author(s):  
Sae-Ra Lee ◽  
Ah-Ram Kim ◽  
Jun-Sub Kim ◽  
Jaebong Kim ◽  
Jae-Yong Lee ◽  
...  
Keyword(s):  
Rat Brain ◽  
Synaptic Vesicle ◽  
Vesicle Membranes

scholarly journals The AP-3 Complex Required for Endosomal Synaptic Vesicle Biogenesis is Associated with a Casein Kinase Ια-Like Isoform

2000 ◽  
Vol 11 (8) ◽  
pp. 2591-2604 ◽  
Author(s):  
Victor V. Faundez ◽  
Regis B. Kelly
Keyword(s):  
Synaptic Vesicle ◽  
Synaptic Vesicles ◽  
Enzymatic Reaction ◽  
Casein Kinase ◽  
Small Gtpase ◽  
Casein Kinase I ◽  
Vesicle Membranes ◽  
Vesicle Biogenesis ◽  
Hinge Domain

The formation of small vesicles is mediated by cytoplasmic coats the assembly of which is regulated by the activity of GTPases, kinases, and phosphatases. A heterotetrameric AP-3 adaptor complex has been implicated in the formation of synaptic vesicles from PC12 endosomes ( Faundez et al., 1998 ). When the small GTPase ARF1 is prevented from hydrolyzing GTP, we can reconstitute AP-3 recruitment to synaptic vesicle membranes in an assembly reaction that requires temperatures above 15°C and the presence of ATP suggesting that an enzymatic step is involved in the coat assembly. We have now found an enzymatic reaction, the phosphorylation of the AP-3 adaptor complex, that is linked with synaptic vesicle coating. Phosphorylation occurs in the β3 subunit of the complex by a kinase similar to casein kinase 1α. The kinase copurifies with neuronal-specific AP-3. In vitro, purified casein kinase I selectively phosphorylates the β3A and β3B subunit at its hinge domain. Inhibiting the kinase hinders the recruitment of AP-3 to synaptic vesicles. The same inhibitors that prevent coat assembly in vitro also inhibit the formation of synaptic vesicles in PC12 cells. The data suggest, therefore, that the mechanism of AP-3-mediated vesiculation from neuroendocrine endosomes requires the phosphorylation of the adaptor complex at a step during or after AP-3 recruitment to membranes.

scholarly journals β-Hydroxybutyrate supports synaptic vesicle cycling but reduces endocytosis and exocytosis in rat brain synaptosomes

2016 ◽  
Vol 93 ◽  
pp. 73-81 ◽  
Author(s):  
Sviatlana V. Hrynevich ◽  
Tatyana V. Waseem ◽  
Audrey Hébert ◽  
Luc Pellerin ◽  
Sergei V. Fedorovich
Keyword(s):  
Rat Brain ◽  
Synaptic Vesicle ◽  
Rat Brain Synaptosomes ◽  
Vesicle Cycling ◽  
Brain Synaptosomes
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