scholarly journals Diallyl sulfide down-regulates polycyclic aromatic hydrocarbon-induced cytochrome P450 1A1 in mouse liver and lung

1996 ◽  
Vol 28 (4) ◽  
pp. 167-172 ◽  
Author(s):  
Young-Sook Hong ◽  
Hae-Young Park ◽  
Sang-Shin Park
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Mouse Liver ◽  
Cytochrome P450 1A1 ◽  
Diallyl Sulfide ◽  
Polycyclic Aromatic

scholarly journals Mechanistic Role of Cytochrome P450 (CYP) 1 Enzymes in Polycyclic Aromatic Hydrocarbon (PAH)‐Mediated Carcinogenesis

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Bhagavatula Moorthy ◽  
Grady Gastelum ◽  
Alex Veith ◽  
Lihua Wang ◽  
Guodong Zhou ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Polycyclic Aromatic

Microsomal cytochrome P450 1A1 dependent monooxygenase activity in guinea pig heart: induction, inhibition, and increased activity by addition of exogenous NADPH–cytochrome P450 reductase

1993 ◽  
Vol 71 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Gordon P. McCallum ◽  
Jennifer E. Horton ◽  
K. Cameron Falkner ◽  
John R. Bend
Keyword(s):  
Cytochrome P450 ◽  
Guinea Pig ◽  
Aromatic Hydrocarbon ◽  
Cytochrome P450 Reductase ◽  
Cytochrome P450 1A1 ◽  
Guinea Pig Heart ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Polycyclic Aromatic ◽  
Nadph Cytochrome P450 Reductase

Characterization of cytochrome P450 1A1 dependent monooxygenases in guinea pig heart revealed low rates of 7-ethoxyresorufin O-deethylation, which are markedly increased (20-fold) by treatment with β-naphthoflavone, a poly cyclic aromatic hydrocarbon. Both 7-ethoxyresorufin O-deethylation and 7-methoxyresorufin O-demethylation were found to be approximately 4-fold higher in microsomes prepared from the ventricle than the atrium of β-naphthoflavone-induced guinea pigs. The low rates of 7-ethoxyresorufin O-deethylation in cardiac microsomes were due, at least in part, to a deficiency of the flavoprotein NADPH–cytochrome P450 reductase; addition of exogenous NADPH–cytochrome P450 reductase dramatically increased 7-ethoxyresorufin O-deethylation in cardiac microsomes of guinea pigs, before and after treatment with β-naphthoflavone. N-Benzyl-1-aminobenzotriazole, a suicide substrate of cytochrome P450 1A1 in guinea pig, was able to inhibit almost all of the 7-ethoxyresorufin O-deethylase and 7-methoxyresorufin O-demethylase activities in polycyclic aromatic hydrocarbon induced guinea pig heart (88 and 71%, respectively), suggesting that cytochrome P450 1A1 coupled to NADPH–cytochrome P450 reductase in these microsomes inactivates itself by a suicidal mechanism. Addition of α-naphthoflavone, an inhibitor of cytochrome P450 1A isozymes, to cardiac microsomes from β-naphthoflavone-induced guinea pigs resulted in greater than 95% inhibition of 7-ethoxyresorufin O-deethylase activity. The biological significance of these low levels of cytochrome P450 1A1 monooxygenase activity in guinea pig heart and their induction by polycyclic aromatic hydrocarbons are not currently understood.Key words: guinea pig, heart, cytochrome P450, NADPH–cytochrome P450 reductase, polycyclic aromatic hydrocarbon.

scholarly journals Mitochondrial Targeting of Cytochrome P450 (CYP) 1B1 and Its Role in Polycyclic Aromatic Hydrocarbon-induced Mitochondrial Dysfunction

2014 ◽  
Vol 289 (14) ◽  
pp. 9936-9951 ◽  
Author(s):  
Seema Bansal ◽  
Adrian N. Leu ◽  
Frank J. Gonzalez ◽  
F. Peter Guengerich ◽  
Anindya Roy Chowdhury ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Mitochondrial Dysfunction ◽  
Mitochondrial Targeting ◽  
Polycyclic Aromatic
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