scholarly journals Diversity of Middle East respiratory syndrome coronaviruses in 109 dromedary camels based on full-genome sequencing, Abu Dhabi, United Arab Emirates

2017 ◽  
Vol 6 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Mohammed Farouk Yusof ◽  
Krista Queen ◽  
Yassir Mohammed Eltahir ◽  
Clinton R Paden ◽  
Zulaikha Mohamed Abdel Hameed Al Hammadi ◽  
...  
Keyword(s):  
Middle East ◽  
United Arab Emirates ◽  
Genome Sequencing ◽  
Middle East Respiratory Syndrome ◽  
Abu Dhabi ◽  
Full Genome ◽  
Full Genome Sequencing ◽  
Dromedary Camels

scholarly journals Prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels in Abu Dhabi Emirate, United Arab Emirates

Virus Genes ◽  
2015 ◽  
Vol 50 (3) ◽  
pp. 509-513 ◽  
Author(s):  
Mohammed F. Yusof ◽  
Yassir M. Eltahir ◽  
Wissam S. Serhan ◽  
Farouk M. Hashem ◽  
Elsaeid A. Elsayed ◽  
...  
Keyword(s):  
Middle East ◽  
United Arab Emirates ◽  
Middle East Respiratory Syndrome ◽  
Abu Dhabi ◽  
Dromedary Camels ◽  
Abu Dhabi Emirate

scholarly journals Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 592
Author(s):  
Stephanie N. Seifert ◽  
Jonathan E. Schulz ◽  
Stacy Ricklefs ◽  
Michael Letko ◽  
Elangeni Yabba ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Middle East ◽  
United Arab Emirates ◽  
Sequence Data ◽  
Case Fatality ◽  
High Sensitivity ◽  
Middle East Respiratory Syndrome ◽  
Full Genome Sequence ◽  
Genome Sequences ◽  
Dromedary Camels

Middle East respiratory syndrome-related coronavirus (MERS-CoV) is a persistent zoonotic pathogen with frequent spillover from dromedary camels to humans in the Arabian Peninsula, resulting in limited outbreaks of MERS with a high case-fatality rate. Full genome sequence data from camel-derived MERS-CoV variants show diverse lineages circulating in domestic camels with frequent recombination. More than 90% of the available full MERS-CoV genome sequences derived from camels are from just two countries, the Kingdom of Saudi Arabia (KSA) and United Arab Emirates (UAE). In this study, we employ a novel method to amplify and sequence the partial MERS-CoV genome with high sensitivity from nasal swabs of infected camels. We recovered more than 99% of the MERS-CoV genome from field-collected samples with greater than 500 TCID50 equivalent per nasal swab from camel herds sampled in Jordan in May 2016. Our subsequent analyses of 14 camel-derived MERS-CoV genomes show a striking lack of genetic diversity circulating in Jordan camels relative to MERS-CoV genome sequences derived from large camel markets in KSA and UAE. The low genetic diversity detected in Jordan camels during our study is consistent with a lack of endemic circulation in these camel herds and reflective of data from MERS outbreaks in humans dominated by nosocomial transmission following a single introduction as reported during the 2015 MERS outbreak in South Korea. Our data suggest transmission of MERS-CoV among two camel herds in Jordan in 2016 following a single introduction event.

scholarly journals Response to Emergence of Middle East Respiratory Syndrome Coronavirus, Abu Dhabi, United Arab Emirates, 2013–2014

2016 ◽  
Vol 22 (7) ◽  
pp. 1162-1168 ◽  
Author(s):  
Farida Ismail Al Hosani ◽  
Kimberly Pringle ◽  
Mariam Al Mulla ◽  
Lindsay Kim ◽  
Huong Pham ◽  
...  
Keyword(s):  
Middle East ◽  
United Arab Emirates ◽  
Middle East Respiratory Syndrome ◽  
Abu Dhabi

scholarly journals A53 MERS-CoV in East African dromedary camels

2019 ◽  
Vol 5 (Supplement_1) ◽  
Author(s):  
Ziqi Zhou ◽  
Daniel K W Chu ◽  
Abraham Ali ◽  
Getnet F Demissié ◽  
Malik Peiris
Keyword(s):  
Middle East ◽  
East Africa ◽  
Arabian Peninsula ◽  
Neutralization Assay ◽  
Middle East Respiratory Syndrome ◽  
Full Genome ◽  
Limited Information ◽  
East African ◽  
Dromedary Camels ◽  
Generation Sequencing

Abstract Human Middle East respiratory syndrome is a zoonotic respiratory disease caused by Middle East respiratory syndrome coronavirus (MERS-CoV) originating from camels in the Arabian Peninsula. While there are a large number of camels in East Africa, often traded to the Arabian Peninsula, no autochthonous human MERS-CoV case is reported in East Africa. Furthermore, there is limited information of MERS-CoV in East Africa. In this study, MERS-CoV in dromedary camels from Ethiopia was detected using RT-qPCR. Next-generation sequencing was used to obtain the full genome of MERS-CoV. MERS-CoV antibodies were also detected through MERS-spike pseudoparticle neutralization assay. Phylogenetic analysis of full-genome sequences and spike-genome antibodies indicates that MERS-CoV in East Africa is genetically distinct from those in the Arabian Peninsula. The results from this study show that MERS-CoV circulating in dromedary camels in East Africa are genetically distinct from those in the Arabian Peninsula. Further studies are needed to evaluate the risk of zoonotic transmission in East Africa.

scholarly journals Serologic Follow-up of Middle East Respiratory Syndrome Coronavirus Cases and Contacts—Abu Dhabi, United Arab Emirates

2018 ◽  
Vol 68 (3) ◽  
pp. 409-418 ◽  
Author(s):  
Farida Ismail Al Hosani ◽  
Lindsay Kim ◽  
Ahmed Khudhair ◽  
Huong Pham ◽  
Mariam Al Mulla ◽  
...  
Keyword(s):  
Middle East ◽  
United Arab Emirates ◽  
Middle East Respiratory Syndrome ◽  
Abu Dhabi

scholarly journals Middle East Respiratory Syndrome Coronavirus during Pregnancy, Abu Dhabi, United Arab Emirates, 2013

2016 ◽  
Vol 22 (3) ◽  
pp. 515-517 ◽  
Author(s):  
Asim Malik ◽  
Karim Medhat El Masry ◽  
Mini Ravi ◽  
Falak Sayed
Keyword(s):  
Middle East ◽  
United Arab Emirates ◽  
Middle East Respiratory Syndrome ◽  
Abu Dhabi

474 Hepatitis B virus genomic markers for hepatocellular carcinoma on full-genome sequencing of 332 patients

2006 ◽  
Vol 44 ◽  
pp. S176-S177
Author(s):  
J.J.Y. Sung ◽  
S.K.W. Tsui ◽  
C.H. Tse ◽  
E.Y.T. Ng ◽  
K.S. Leung ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Genome Sequencing ◽  
Full Genome ◽  
Full Genome Sequencing ◽  
B Virus ◽  
Genomic Markers

scholarly journals A Half-Day Genome Sequencing Protocol for Middle East Respiratory Syndrome Coronavirus

2021 ◽  
Vol 12 ◽  
Author(s):  
Kwan Woo Kim ◽  
Sungmi Choi ◽  
Su-Kyoung Shin ◽  
Imchang Lee ◽  
Keun Bon Ku ◽  
...  
Keyword(s):  
Public Health ◽  
Middle East ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Middle East Respiratory Syndrome ◽  
Universal Primers ◽  
Clinical Samples ◽  
Primary Concern ◽  
Whole Genome ◽  
Serum Samples

Recent coronavirus (CoV) outbreaks, including that of Middle East respiratory syndrome (MERS), have presented a threat to public health worldwide. A primary concern in these outbreaks is the extent of mutations in the CoV, and the content of viral variation that can be determined only by whole genome sequencing (WGS). We aimed to develop a time efficient WGS protocol, using universal primers spanning the entire MERS-CoV genome. MERS and synthetic Neoromicia capensis bat CoV genomes were successfully amplified using our developed PCR primer set and sequenced with MinION. All experimental and analytical processes took 6 h to complete and were also applied to synthetic animal serum samples, wherein the MERS-CoV genome sequence was completely recovered. Results showed that the complete genome of MERS-CoV and related variants could be directly obtained from clinical samples within half a day. Consequently, this method will contribute to rapid MERS diagnosis, particularly in future CoV epidemics.

Sign in / Sign up

Export Citation Format

Share Document