scholarly journals Antisense regulation by transposon‐derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus

EMBO Reports ◽  
2013 ◽  
Vol 14 (6) ◽  
pp. 527-533 ◽  
Author(s):  
Birgit Märtens ◽  
Salim Manoharadas ◽  
David Hasenöhrl ◽  
Andrea Manica ◽  
Udo Bläsi
Keyword(s):  
Sulfolobus Solfataricus ◽  
Hyperthermophilic Archaeon ◽  
Antisense Regulation

scholarly journals Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus

2006 ◽  
Vol 62 (4) ◽  
pp. 1076-1089 ◽  
Author(s):  
Pamela Walter ◽  
Franziska Klein ◽  
Esben Lorentzen ◽  
Anne Ilchmann ◽  
Gabriele Klug ◽  
...  
Keyword(s):  
Sulfolobus Solfataricus ◽  
Hyperthermophilic Archaeon

scholarly journals Production of Recombinant and Tagged Proteins in the Hyperthermophilic Archaeon Sulfolobus solfataricus

2006 ◽  
Vol 72 (1) ◽  
pp. 102-111 ◽  
Author(s):  
S.-V. Albers ◽  
M. Jonuscheit ◽  
S. Dinkelaker ◽  
T. Urich ◽  
A. Kletzin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Shuttle Vector ◽  
Sulfolobus Solfataricus ◽  
Inducible Promoter ◽  
Single Step ◽  
Vector System ◽  
Hyperthermophilic Archaea ◽  
Model Organisms ◽  
Hyperthermophilic Archaeon ◽  
High Level

ABSTRACT Many systems are available for the production of recombinant proteins in bacterial and eukaryotic model organisms, which allow us to study proteins in their native hosts and to identify protein-protein interaction partners. In contrast, only a few transformation systems have been developed for archaea, and no system for high-level gene expression existed for hyperthermophilic organisms. Recently, a virus-based shuttle vector with a reporter gene was developed for the crenarchaeote Sulfolobus solfataricus, a model organism of hyperthermophilic archaea that grows optimally at 80°C (M. Jonuscheit, E. Martusewitsch, K. M. Stedman, and C. Schleper, Mol. Microbiol. 48:1241-1252, 2003). Here we have refined this system for high-level gene expression in S. solfataricus with the help of two different promoters, the heat-inducible promoter of the major chaperonin, thermophilic factor 55, and the arabinose-inducible promoter of the arabinose-binding protein AraS. Functional expression of heterologous and homologous genes was demonstrated, including production of the cytoplasmic sulfur oxygenase reductase from Acidianus ambivalens, an Fe-S protein of the ABC class from S. solfataricus, and two membrane-associated ATPases potentially involved in the secretion of proteins. Single-step purification of the proteins was obtained via fused His or Strep tags. To our knowledge, these are the first examples of the application of an expression vector system to produce large amounts of recombinant and also tagged proteins in a hyperthermophilic archaeon.

The β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: enzyme activity and conformational dynamics at temperatures above 100°C

1999 ◽  
Vol 81 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Sabato D’Auria ◽  
Roberto Nucci ◽  
Mose’ Rossi ◽  
Ignacy Gryczynski ◽  
Zgymunt Gryczynski ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Conformational Dynamics ◽  
Sulfolobus Solfataricus ◽  
Hyperthermophilic Archaeon

scholarly journals A Heterotrimeric PCNA in the Hyperthermophilic Archaeon Sulfolobus solfataricus

2003 ◽  
Vol 11 (1) ◽  
pp. 275-282 ◽  
Author(s):  
Isabelle Dionne ◽  
Ravi K Nookala ◽  
Stephen P Jackson ◽  
Aidan J Doherty ◽  
Stephen D Bell
Keyword(s):  
Sulfolobus Solfataricus ◽  
Hyperthermophilic Archaeon

scholarly journals High Spontaneous Mutation Rate in the Hyperthermophilic Archaeon Sulfolobus solfataricus Is Mediated by Transposable Elements

2000 ◽  
Vol 182 (9) ◽  
pp. 2574-2581 ◽  
Author(s):  
Erika Martusewitsch ◽  
Christoph W. Sensen ◽  
Christa Schleper
Keyword(s):  
Transposable Elements ◽  
Spontaneous Mutation ◽  
Sulfolobus Solfataricus ◽  
Viable Cell ◽  
Spontaneous Mutation Rate ◽  
Hyperthermophilic Archaeon ◽  
Is Element ◽  
Genes Encoding ◽  
Genetic Transformation System ◽  
Spontaneous Mutants

ABSTRACT We have isolated uracil-auxotrophic mutants of the hyperthermophilic archaeon Sulfolobus solfataricus in order to explore the genomic stability and mutational frequencies of this organism and to identify complementable recipients for a selectable genetic transformation system. Positive selection of spontaneous mutants resistant to 5-fluoroorotate yielded uracil auxotrophs with frequencies of between 10−4 and 10−5 per sensitive, viable cell. Four different, nonhomologous insertion sequences (ISs) were identified at different positions within the chromosomal pyrEF locus of these mutants. They ranged in size from 1,058 to 1,439 bp and possessed properties typical of known transposable elements, i.e., terminal inverted repeats, flanking duplicated target sequences, and putative transposase genes encoding motifs that are indicative of the IS4-IS5 IS element families. Between 12 and 25 copies of each IS element were found in chromosomal DNAs by Southern analyses. While characteristic fingerprint patterns created by IS element-specific probes were observed with genomic DNA of different S. solfataricusstrains, no homologous sequences were identified in DNA of other well-characterized strains of the order Sulfolobales.

scholarly journals Template-dependent polymerization across discontinuous templates by the heterodimeric primase from the hyperthermophilic archaeon Sulfolobus solfataricus

2011 ◽  
Vol 40 (8) ◽  
pp. 3470-3483 ◽  
Author(s):  
Jinchuan Hu ◽  
Li Guo ◽  
Kangyun Wu ◽  
Bing Liu ◽  
Shiwei Lang ◽  
...  
Keyword(s):  
Sulfolobus Solfataricus ◽  
Hyperthermophilic Archaeon
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