scholarly journals Transcriptional activation of DNA‐dependent protein kinase catalytic subunit gene expression by oestrogen receptor‐α

EMBO Reports ◽  
2010 ◽  
Vol 11 (3) ◽  
pp. 208-213 ◽  
Author(s):  
Senad Medunjanin ◽  
Sönke Weinert ◽  
David Poitz ◽  
Alexander Schmeisser ◽  
Ruth H Strasser ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Transcriptional Activation ◽  
Oestrogen Receptor ◽  
Catalytic Subunit ◽  
Subunit Gene ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Oestrogen Receptor Α

CsA-sensitive purine-box transcriptional regulator in bronchial epithelial cells contains NF45, NF90, and Ku

1998 ◽  
Vol 275 (6) ◽  
pp. L1164-L1172 ◽  
Author(s):  
Yosuke Aoki ◽  
Guohua Zhao ◽  
Daoming Qiu ◽  
Lingfang Shi ◽  
Peter N. Kao
Keyword(s):  
Protein Kinase ◽  
Dna Binding ◽  
Dna Sequence ◽  
Transcriptional Activation ◽  
Nuclear Dna ◽  
Transcriptional Regulator ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Bronchial Epithelial ◽  
Dependent Protein

Human bronchial epithelial (HBE) cells express interleukin (IL)-2 [Y. Aoki, D. Qiu, A. Uyei, and P. N. Kao. Am. J. Physiol. 272 ( Lung Cell. Mol. Physiol. 16): L276–L286, 1997]. 16HBE-transformed cells contain constitutive and inducible nuclear DNA-binding activity for the purine-box/nuclear factor (NF) of activated T cell (NFAT) target DNA sequence in the human IL-2 enhancer. Transcriptional activation through the purine-box DNA sequence requires stimulation with phorbol 12-myristate 13-acetate + ionomycin, and this activation is inhibited by cyclosporin A. Immunohistochemical staining of 16HBE cells demonstrates nuclear expression of the purine-box DNA-binding proteins NF45 and NF90 and no expression of NFATp or NFATc. NF90 and NF45 associate with the DNA-dependent protein kinase catalytic subunit and the DNA-targeting subunits Ku80 and Ku70 (N. S. Ting, P. N. Kao, D. W. Chan, L. G. Lintott, and S. P. Lees-Miller. J. Biol. Chem. 273: 2136–2145, 1998). Antibodies to Ku potently inhibit the purine-box DNA-binding complex. The purine-box transcriptional regulator in 16HBE cells likely comprises NF45, NF90, Ku80, Ku70, and the DNA-dependent protein kinase catalytic subunit.

cDNA Cloning and Gene Expression of Human Type Iα cGMP-Dependent Protein Kinase

Hypertension ◽  
1996 ◽  
Vol 27 (3) ◽  
pp. 552-557 ◽  
Author(s):  
Naohisa Tamura ◽  
Hiroshi Itoh ◽  
Yoshihiro Ogawa ◽  
Osamu Nakagawa ◽  
Masaki Harada ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Kinase ◽  
Cdna Cloning ◽  
Dependent Protein Kinase ◽  
Human Type ◽  
Cgmp Dependent Protein Kinase ◽  
Dependent Protein
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