scholarly journals T-cell antigen receptor triggering and lipid rafts: a matter of space and time scales. Talking Point on the involvement of lipid rafts in T-cell activation

EMBO Reports ◽  
2008 ◽  
Vol 9 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Hai-Tao He ◽  
Didier Marguet
Keyword(s):  
T Cell ◽  
Lipid Rafts ◽  
Time Scales ◽  
T Cell Activation ◽  
Cell Activation ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Space And Time

scholarly journals Molecular Characterization of a Fully Human Chimeric T-Cell Antigen Receptor for Tumor-Associated Antigen EpCAM

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Naoto Shirasu ◽  
Hiromi Yamada ◽  
Hirotomo Shibaguchi ◽  
Motomu Kuroki ◽  
Masahide Kuroki
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Molecular Mechanisms ◽  
Antigen Receptor ◽  
Target Cells ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen

The transduction of T cells to express chimeric T-cell antigen receptor (CAR) is an attractive strategy for adaptive immunotherapy for cancer, because the CAR can redirect the recognition specificity of T cells to tumor-associated antigens (TAAs) on the surface of target cells, thereby avoiding the limitations of HLA restriction. However, there are considerable problems with the clinical application of CAR, mostly due to its xenogeneic components, which could be immunogenic in humans. Moreover, while extensive studies on the CARs have been performed, the detailed molecular mechanisms underlying the activation of CAR-grafted T cells remain unclear. In order to eliminate potential immunogenicity and investigate the molecular basis of the CAR-mediated T-cell activation, we constructed a novel CAR (CAR57-28ζ) specific for one of the most important TAAs, epithelial cell adhesion molecule (EpCAM), using only human-derived genes. We revealed that in Jurkat T cells, lentivirally expressed CAR57-28ζ can transmit the T-cell-activating signals sufficient to induce IL-2 production upon EpCAM stimulation. An immunofluorescent analysis clearly showed that the CAR57-28ζ induces the formation of signaling clusters containing endogenous CD3ζ at the CAR/EpCAM interaction interface. These results suggest that this CAR gene may be safely and effectively applied for adaptive T-cell immunotherapy.

Use of adoptive transfer of T-cell antigen-receptor-transgenic T cells for the study of T-cell activation in vivo

1997 ◽  
Vol 156 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Kathryn A. Pape ◽  
Elizabeth R. Kearney ◽  
Alexander Khoruts ◽  
Anna Mondino ◽  
Rebecca Merica ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Adoptive Transfer ◽  
Cell Activation ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen

The Interchain Disulfide Linkage of T-Cell Antigen Receptor-α and -β Chains Is a Prerequisite for T-Cell Activation

1998 ◽  
Vol 190 (2) ◽  
pp. 101-111 ◽  
Author(s):  
Zhanguo Li ◽  
Weiping Wu ◽  
Owen Kemp ◽  
Melinda Stephen ◽  
Nicholas Manolios
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Disulfide Linkage ◽  
Cell Antigen Receptor ◽  
Cell Antigen

scholarly journals Removal of C-Terminal Src Kinase from the Immune Synapse by a New Binding Protein

2005 ◽  
Vol 25 (6) ◽  
pp. 2227-2241 ◽  
Author(s):  
Souad Rahmouni ◽  
Torkel Vang ◽  
Andres Alonso ◽  
Scott Williams ◽  
Marianne van Stipdonk ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Interleukin 2 ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Intracellular Location ◽  
Immune Synapse ◽  
Cell Antigen Receptor ◽  
Cell Antigen

ABSTRACT The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells. Engagement of the T-cell antigen receptor results in a removal of Csk from the lipid raft-associated transmembrane protein PAG/Cbp. Instead, Csk becomes associated with an ∼72-kDa tyrosine-phosphorylated protein, which we identify here as G3BP, a phosphoprotein reported to bind the SH3 domain of Ras GTPase-activating protein. G3BP reduced the ability of Csk to phosphorylate Lck at Y505 by decreasing the amount of Csk in lipid rafts. As a consequence, G3BP augmented T-cell activation as measured by interleukin-2 gene activation. Conversely, elimination of endogenous G3BP by RNA interference increased Lck Y505 phosphorylation and reduced TCR signaling. In antigen-specific T cells, endogenous G3BP moved into a intracellular location adjacent to the immune synapse, but deeper inside the cell, upon antigen recognition. Csk colocalization with G3BP occurred in this “parasynaptic” location. We conclude that G3BP is a new player in T-cell-antigen receptor signaling and acts to reduce the amount of Csk in the immune synapse.

scholarly journals Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering

2003 ◽  
Vol 369 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Luca BINI ◽  
Sonia PACINI ◽  
Sabrina LIBERATORI ◽  
Silvia VALENSIN ◽  
Michela PELLEGRINI ◽  
...  
Keyword(s):  
T Cell ◽  
Lipid Rafts ◽  
Antigen Receptor ◽  
Temporal Analysis ◽  
Membrane Microdomains ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Dynamic Structures ◽  
Tcr Signalling

Signalling by immunoreceptors is orchestrated at specific plasma membrane microdomains, referred to as lipid rafts. Here we present a proteomics approach to the temporal analysis of protein association with lipid rafts following T-cell antigen receptor (TCR) triggering. We show that TCR engagement promotes the temporally regulated recruitment of proteins participating in the TCR signalling cascade to lipid rafts. Furthermore, TCR triggering results in profound modifications in the composition of lipid rafts involving a number of proteins associated either directly or indirectly with both plasma and intracellular membranes. Raft-associated proteins can be clustered according to their temporal profile of raft association. The data identify lipid rafts as highly dynamic structures and reveal a dramatic impact of surface TCR triggering not only on components of the TCR signalling machinery but also on proteins implicated in a number of diverse cellular processes.

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