scholarly journals Repertoire comparison of the B-cell receptor-encoding loci in humans and rhesus macaques by next-generation sequencing

2016 ◽  
Vol 5 (7) ◽  
pp. e93 ◽  
Author(s):  
Vladimir Vigdorovich ◽  
Brian G Oliver ◽  
Sara Carbonetti ◽  
Nicholas Dambrauskas ◽  
Miles D Lange ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
B Cell ◽  
Rhesus Macaques ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Next Generation Sequencing Reveals Skewing of the T and B Cell Receptor Repertoires in Patients with Wiskott–Aldrich Syndrome

2014 ◽  
Vol 5 ◽  
Author(s):  
Amy E. O’Connell ◽  
Stefano Volpi ◽  
Kerry Dobbs ◽  
Claudia Fiorini ◽  
Erdyni Tsitsikov ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
B Cell ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Immunoglobulin G4+ clones identified by next-generation sequencing dominate the B cell receptor repertoire in immunoglobulin G4 associated cholangitis

Hepatology ◽  
2013 ◽  
Vol 57 (6) ◽  
pp. 2390-2398 ◽  
Author(s):  
Lucas J. Maillette de Buy Wenniger ◽  
Marieke E. Doorenspleet ◽  
Paul L. Klarenbeek ◽  
Joanne Verheij ◽  
Frank Baas ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
B Cell ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Next Generation ◽  
Immunoglobulin G4 ◽  
Receptor Repertoire ◽  
Generation Sequencing ◽  
Cell Receptor Repertoire

scholarly journals Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease

Immunity ◽  
2020 ◽  
Vol 53 (2) ◽  
pp. 442-455.e4 ◽  
Author(s):  
Christoph Schultheiß ◽  
Lisa Paschold ◽  
Donjete Simnica ◽  
Malte Mohme ◽  
Edith Willscher ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
B Cell ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Next Generation ◽  
Severity Of Disease ◽  
Generation Sequencing

scholarly journals Immortalization and Functional Screening of Natively Paired Human T Cell Receptor Repertoires

2021 ◽  
Author(s):  
Ahmed S Fahad ◽  
Cheng Yu Chung ◽  
Sheila N. Lopez Acevedo ◽  
Nicoleen Boyle ◽  
Bharat Madan ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Functional Screening ◽  
Next Generation ◽  
Sequencing Technologies ◽  
Human T Cell ◽  
Screening Platform ◽  
Generation Sequencing

Functional analyses of the T cell receptor (TCR) landscape can reveal critical information about protection from disease and molecular responses to vaccines. However, it has proven difficult to combine advanced next-generation sequencing technologies with methods to decode the peptide-major histocompatibility complex (pMHC) specificity of individual TCRs. Here we developed a new high-throughput approach to enable repertoire-scale functional evaluations of natively paired TCRs. In particular, we leveraged the immortalized nature of physically linked TCRα:β amplicon libraries to analyze binding against multiple recombinant pMHCs on a repertoire scale. To exemplify the utility of this approach, we also performed affinity-based functional mapping in conjunction with quantitative next-generation sequencing to track antigen- specific TCRs. These data successfully validated a new immortalization and screening platform to facilitate detailed molecular analyses of human TCRs against diverse antigen targets associated with health, vaccination, or disease.

scholarly journals Responding kinetic of B-cell receptor repertoire to the Toll-like receptor 7/8 stimulation in non-human primates

2021 ◽  
Author(s):  
Shiyu Wang ◽  
Judith Mandl ◽  
Mark Feinberg ◽  
Michael Citron ◽  
Nitin K. Saksena ◽  
...  
Keyword(s):  
B Cell ◽  
Mutation Rate ◽  
Cell Activation ◽  
Rhesus Macaques ◽  
Low Frequency ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Receptor Repertoire ◽  
Cell Immunity ◽  
Repertoire Diversity

TLR7 and 8 regulate B cell immunity, but the precise details of the mechanism are still unclear. Here, we studied the kinetics of both heavy and light chains (IgKL) of B-cell receptor (BCR) repertoire responding to the TLR7/8 stimulation in two geniuses of non-human primates (NHPs), African green monkeys (AGMs) and rhesus macaques (RMs). We evaluated the activation of lymphocytes by flow cytometry and studied characteristics of BCR repertoire in terms of gene usage, repertoire diversity, and the number of lineages. Although AGMs had a weaker activation than RMs, and a different responding kinetic, both AGMs and RMs presented an increased IgKL repertoire diversity and lineages expansion. It suggested that the responding time rather than initiation of TLR7/8-induced IgKL repertoire response related to B cell activation. Expanded IgKL lineages with frequency from 0.001% to 1% had an elevated mutation rate and expanded IgH lineages used more IgA/G/E, suggesting that the TLR7/8 stimulation expanded low-frequent but high-mutated lineages. Besides, most of expanded IgKL lineages were lambda isotype. In conclusion, TLR7/8 selectively expands IgKL lineages with a high mutation rate, low frequency, and lambda isotype. The selective effect of TLR7/8 on BCR repertoire allows TLR7/8 agonists to be adjuvant for selectively accelerating antibody maturation.

scholarly journals Preparing Unbiased T-Cell Receptor and Antibody cDNA Libraries for the Deep Next Generation Sequencing Profiling

2013 ◽  
Vol 4 ◽  
Author(s):  
Ilgar Z. Mamedov ◽  
Olga V. Britanova ◽  
Ivan V. Zvyagin ◽  
Maria A. Turchaninova ◽  
Dmitriy A. Bolotin ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cdna Libraries ◽  
Next Generation ◽  
Generation Sequencing
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