scholarly journals Comparative Proteomic Analysis of Whole-Gut Lavage Fluid and Pancreatic Juice Reveals a Less Invasive Method of Sampling Pancreatic Secretions

2016 ◽  
Vol 7 (5) ◽  
pp. e174 ◽  
Author(s):  
Jana M Rocker ◽  
Marcus C Tan ◽  
Lee W Thompson ◽  
Carlo M Contreras ◽  
Jack A DiPalma ◽  
...  
Keyword(s):  
Pancreatic Juice ◽  
Proteomic Analysis ◽  
Lavage Fluid ◽  
Less Invasive ◽  
Comparative Proteomic Analysis ◽  
Invasive Method ◽  
Gut Lavage

Comparative proteomic analysis of human pancreatic juice: Methodological study

PROTEOMICS ◽  
2007 ◽  
Vol 7 (8) ◽  
pp. 1345-1355 ◽  
Author(s):  
Lu Zhou ◽  
ZhaoHui Lu ◽  
AiMing Yang ◽  
RuiXue Deng ◽  
CanRong Mai ◽  
...  
Keyword(s):  
Pancreatic Juice ◽  
Proteomic Analysis ◽  
Methodological Study ◽  
Comparative Proteomic Analysis

Whole Gut Lavage Fluid Analysis: A Minimally Invasive Method for Study of Mucosal Immunity and Inflammation

2003 ◽  
pp. 257-277 ◽  
Author(s):  
Subrata Ghosh ◽  
Anna Dahele ◽  
Hazel E. Drummond ◽  
Syed S. Hoque ◽  
Kenneth Humphreys ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Mucosal Immunity ◽  
Lavage Fluid ◽  
Fluid Analysis ◽  
Invasive Method ◽  
Gut Lavage

scholarly journals Comparative Proteomic Analysis of Rat Bronchoalveolar Lavage Fluid after Exposure to Zinc Oxide Nanoparticles

2017 ◽  
Vol 6 (2) ◽  
pp. S0066-S0066 ◽  
Author(s):  
Yu-Min Juang ◽  
Han-Ju Chien ◽  
Cheng-Yu Yang ◽  
Hsiao-Chien Yeh ◽  
Tsun-Jen Cheng ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Bronchoalveolar Lavage ◽  
Proteomic Analysis ◽  
Zinc Oxide Nanoparticles ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Oxide Nanoparticles ◽  
Comparative Proteomic Analysis

Bronchoalveolar Lavage Fluid in Children: Comparative Proteomic Analysis in Infectious and Non-Infectious Lung Disease

2018 ◽  
Vol 31 (1) ◽  
pp. 15-23
Author(s):  
Gloria Pelizzo ◽  
Marco Fumagalli ◽  
Carmine Tinelli ◽  
Annalisa De Silvestri ◽  
Riccardo Albertini ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Lung Disease ◽  
Proteomic Analysis ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Comparative Proteomic Analysis

An ELISA to Measure Mucosal IgA Specific forBacteroidesSurface Antigens in Whole Gut Lavage Fluid

1994 ◽  
Vol 8 (3) ◽  
Author(s):  
I. R. Poxtin ◽  
C. J. Myers ◽  
A. Johnstone ◽  
T. A. Drudy ◽  
A. Ferguson
Keyword(s):  
Lavage Fluid ◽  
Gut Lavage

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

scholarly journals Development of Injectable Polydactyly-Derived Chondrocyte Sheets

2021 ◽  
Vol 22 (6) ◽  
pp. 3198
Author(s):  
Shiho Wasai ◽  
Eriko Toyoda ◽  
Takumi Takahashi ◽  
Miki Maehara ◽  
Eri Okada ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cartilage Damage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Invasive Treatment ◽  
Experimental Conditions ◽  
Humoral Factors ◽  
Less Invasive ◽  
Cell Counts ◽  
Invasive Method ◽  
Injection Control

We are conducting a clinical study of the use of allogeneic polydactyly-derived chondrocyte sheets (PD sheets) for the repair of articular cartilage damage caused by osteoarthritis. However, the transplantation of PD sheets requires highly invasive surgery. To establish a less invasive treatment, we are currently developing injectable fragments of PD sheets (PD sheets-mini). Polydactyly-derived chondrocytes were seeded in RepCell™ or conventional temperature-responsive inserts and cultured. Cell counts and viability, histology, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), and flow cytometry were used to characterize PD sheets-mini and PD sheets collected from each culture. To examine the effects of injection on cell viability, PD sheets-mini were tested in four experimental conditions: non-injection control, 18 gauge (G) needle, 23G needle, and syringe only. PD sheets-mini produced similar amounts of humoral factors as PD sheets. No histological differences were observed between PD sheets and PD sheets-mini. Except for COL2A1, expression of cartilage-related genes did not differ between the two types of PD sheet. No significant differences were observed between injection conditions. PD sheets-mini have characteristics that resemble PD sheets. The cell viability of PD sheets-mini was not significantly affected by needle gauge size. Intra-articular injection may be a feasible, less invasive method to transplant PD sheets-mini.

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