scholarly journals Fission yeast telomere-binding protein Taz1 is a functional but not a structural counterpart of human TRF1 and TRF2

Cell Research ◽  
2015 ◽  
Vol 25 (7) ◽  
pp. 881-884 ◽  
Author(s):  
Wei Deng ◽  
Jian Wu ◽  
Feng Wang ◽  
Junko Kanoh ◽  
Pierre-Marie Dehe ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Binding Protein ◽  
Telomere Binding Protein ◽  
Yeast Telomere

scholarly journals The C Terminus of the Major Yeast Telomere Binding Protein Rap1p Enhances Telomere Formation

1998 ◽  
Vol 18 (3) ◽  
pp. 1284-1295 ◽  
Author(s):  
Alo Ray ◽  
Kurt W. Runge
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reverse Transcriptase ◽  
Binding Site ◽  
Binding Protein ◽  
Telomerase Activity ◽  
Telomerase Rna ◽  
Constant Length ◽  
C Terminus ◽  
Telomere Binding Protein ◽  
Yeast Telomere

ABSTRACT The telomeres of most organisms consist of short repeated sequences that can be elongated by telomerase, a reverse transcriptase complex that contains its own RNA template for the synthesis of telomere repeats. In Saccharomyces cerevisiae, the RAP1gene encodes the major telomere binding protein Rap1p. Here we use a quantitative telomere formation assay to demonstrate that Rap1p C termini can enhance telomere formation more than 30-fold when they are located at internal sites. This stimulation is distinct from protection from degradation. Enhancement of formation required the gene for telomerase RNA but not Sir1p, Sir2p, Sir3p, Sir4p, Tel1p, or the Rif1p binding site in the Rap1p C terminus. Our data suggest that Rap1p C termini enhance telomere formation by attracting or increasing the activity of telomerase near telomeres. Earlier work suggests that Rap1p molecules at the chromosome terminus inhibit the elongation of long telomeres by blocking the access of telomerase. Our results suggest a model where a balance between internal Rap1p increasing telomerase activity and Rap1p at the termini of long telomeres controlling telomerase access maintains telomeres at a constant length.

scholarly journals Fission Yeast Taz1 and RPA Are Synergistically Required to Prevent Rapid Telomere Loss

2007 ◽  
Vol 18 (6) ◽  
pp. 2378-2387 ◽  
Author(s):  
Tatsuya Kibe ◽  
Yuuki Ono ◽  
Koichiro Sato ◽  
Masaru Ueno
Keyword(s):  
Fission Yeast ◽  
Binding Protein ◽  
Protein A ◽  
Telomere Maintenance ◽  
Helicase Domain ◽  
Recq Helicase ◽  
Telomeric Dna ◽  
Telomere Binding Protein ◽  
Recombination Repair ◽  
Telomere Loss

The telomere complex must allow nucleases and helicases to process chromosome ends to make them substrates for telomerase, while preventing these same activities from disrupting chromosome end-protection. Replication protein A (RPA) binds to single-stranded DNA and is required for DNA replication, recombination, repair, and telomere maintenance. In fission yeast, the telomere binding protein Taz1 protects telomeres and negatively regulates telomerase. Here, we show that taz1-d rad11-D223Y double mutants lose their telomeric DNA, indicating that RPA (Rad11) and Taz1 are synergistically required to prevent telomere loss. Telomere loss in the taz1-d rad11-D223Y double mutants was suppressed by additional mutation of the helicase domain in a RecQ helicase (Rqh1), or by overexpression of Pot1, a single-strand telomere binding protein that is essential for protection of chromosome ends. From our results, we propose that in the absence of Taz1 and functional RPA, Pot1 cannot function properly and the helicase activity of Rqh1 promotes telomere loss. Our results suggest that controlling the activity of Rqh1 at telomeres is critical for the prevention of genomic instability.

The Telomere-Binding Protein Taz1p as a Target for Modification by a SUMO-1 Homologue in Fission Yeast

2005 ◽  
Vol 43 (3-4) ◽  
pp. 103-117 ◽  
Author(s):  
K. Spink ◽  
J. C. Y. Ho ◽  
K. Tanaka ◽  
F. Z. Watts ◽  
A. Chambers
Keyword(s):  
Fission Yeast ◽  
Binding Protein ◽  
Telomere Binding Protein

scholarly journals Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast

2012 ◽  
Vol 26 (18) ◽  
pp. 2050-2062 ◽  
Author(s):  
A. Tazumi ◽  
M. Fukuura ◽  
R. Nakato ◽  
A. Kishimoto ◽  
T. Takenaka ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Binding Protein ◽  
Replication Timing ◽  
Late Replication ◽  
Replication Origins ◽  
Telomere Binding Protein
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