scholarly journals Modeling sarcomagenesis using multipotent mesenchymal stem cells

Cell Research ◽  
2011 ◽  
Vol 22 (1) ◽  
pp. 62-77 ◽  
Author(s):  
Rene Rodriguez ◽  
Ruth Rubio ◽  
Pablo Menendez
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals p130 And pRb in the Maintenance of Transient Quiescence of Mesenchymal Stem Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Boris Popov ◽  
Nikolai Petrov ◽  
Vladimir Ryabov ◽  
Igor Evsyukov
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Critical Role ◽  
Growth Curves ◽  
State Level ◽  
Gel Shift Assay ◽  
Multipotent Mesenchymal Stem Cells ◽  
Cycle Regulation ◽  
And Control

An effective regulation of quiescence plays a key role in the differentiation, plasticity, and prevention of stem cells from becoming malignant. The state of quiescence is being controlled by the pRb family proteins which show overlapping functions in cell cycle regulation; however, their roles in controlling the proliferation of mesenchymal stem cells (MSCs) remain to be understood. This study investigated the regulation of transient quiescence using growth curves, proliferation assay, the cytometric evaluation of cell cycle, Western blotting, and the electromobility gel shift assay (EMSA) on synchronized MSCs of the C3H10Т1/2 and control cells with different statuses of pRb proteins. It has been found that functional steady-state level of p130 but not pRb plays a critical role for entering, exiting, and maintenance of transient quiescence in multipotent mesenchymal stem cells.

scholarly journals Immunomodulatory properties of human adult and fetal multipotent mesenchymal stem cells

2011 ◽  
Vol 18 (1) ◽  
pp. 49 ◽  
Author(s):  
Pei-Min Chen ◽  
Men-Luh Yen ◽  
Ko-Jiunn Liu ◽  
Huey-Kang Sytwu ◽  
B-Linju Yen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Adult ◽  
Immunomodulatory Properties ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Isolation and characterization of multipotent mesenchymal stem cells in nasal polyps

2014 ◽  
Vol 240 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Jung-Sun Cho ◽  
Joo-Hoo Park ◽  
Ju-Hyung Kang ◽  
Sung Eun Kim ◽  
Il-Ho Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nasal Polyps ◽  
Isolation And Characterization ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Adipose tissue: A natural resource for multipotent mesenchymal stem cells with potential translation to trigerminal layers

2018 ◽  
Vol 51 (02) ◽  
pp. 177-181 ◽  
Author(s):  
Bhaskar Vyas ◽  
Atul Shah ◽  
Anant Marathe ◽  
Rajni Vyas ◽  
Ramesh Bhonde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Natural Resource ◽  
Pure Culture ◽  
Science Research ◽  
Enzymatic Digestion ◽  
Translation Study ◽  
Vascular Factor ◽  
Multipotent Mesenchymal Stem Cells

ABSTRACTBackground: The article reports basic science research that establishes that adipose tissue (AT)-derived mesenchymal stem cells (MSCs) have a potential to transgerminal translation. Study Design: MSC confirmation was obtained by phenotypic spindle-shaped cells as well as with four positive and three negative markers. The translineage translation of adipose-derived MSCs (ADMSCs) was established. Materials and Methods: The lipoaspirate was subjected to enzymatic digestion with collagenase. Stromal vascular factor (SVF) was isolated. With two passages, pure culture of ADMSCs was obtained. They were translated to all the three germinal layers. Results: AT-derived SVF contains ~30% MSCs. They are capable of being translated into endoderm, mesoderm and ectoderm. Conclusion: AT is a rich source for MSCs, with immense research possibilities for regeneration and rejuvenation.

scholarly journals Multipotent mesenchymal stem cells in lung fibrosis

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0181946 ◽  
Author(s):  
Katrin E. Hostettler ◽  
Amiq Gazdhar ◽  
Petra Khan ◽  
Spasenija Savic ◽  
Luca Tamo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lung Fibrosis ◽  
Multipotent Mesenchymal Stem Cells

scholarly journals Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Rui Zhang ◽  
Nan Wang ◽  
Man Zhang ◽  
Li-Nan Zhang ◽  
Zhi-Xia Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Network Formation ◽  
Umbilical Vein ◽  
Response To Treatment ◽  
Luciferase Reporter ◽  
Rock Inhibitor ◽  
Multipotent Mesenchymal Stem Cells ◽  
Umbilical Vein Endothelial Cells ◽  
Endothelial Growth Factor

Mesenchymal stem cells (MSCs) are known to undergo endothelial differentiation in response to treatment with vascular endothelial growth factor (VEGF), but their angiogenic ability is poorly characterized. In the present study, we aimed to further investigate the role of Rho/MRTF-A in angiogenesis by MSCs and the effect of the Rho/MRTF-A pathway on the expression of integrinsα1β1 andα5β1, which are known to mediate physiological and pathological angiogenesis. Our results showed that increased expression ofα1,α5, andβ1 was observed during angiogenesis of differentiated MSCs, and the Rho/MRTF-A signaling pathway was demonstrated to be involved in regulating the expression of integrinsα1,α5, andβ1. Luciferase reporter assay and ChIP assay determined that MRTF-A could bind to and transactivate the integrinα1 andα5 promoters. Treatment with the Rho inhibitor C3 transferase, the Rho-associated protein kinase (ROCK) inhibitor Y27632 or with shMRTF-A inhibited both the upregulation ofα1,α5, andβ1 as well as angiogenesis. Furthermore, in human umbilical vein endothelial cells (HUVECs), MRTF-A deletion led to marked reductions in cell migration and vessel network formation compared with the control. These data demonstrate that Rho/MRTF-A signaling is an important mediator that controls integrin gene expression during MSC-mediated angiogenic processes.

Identification of variables that optimize isolation and culture of multipotent mesenchymal stem cells from equine umbilical-cord blood

2009 ◽  
Vol 70 (12) ◽  
pp. 1526-1535 ◽  
Author(s):  
Elizabeth M. Schuh ◽  
Michael S. Friedman ◽  
Danielle D. Carrade ◽  
Junzhi Li ◽  
Darren Heeke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Multipotent Mesenchymal Stem Cells

The transplantation of multipotent mesenchymal stem cells in rats with acute rhabdomyolysiscaused kidney tubular necrosis

2015 ◽  
Vol 21 (31) ◽  
pp. 40-44
Author(s):  
Еналдиева ◽  
Dinara Enaldieva ◽  
Дзахова ◽  
Galina Dzakhova ◽  
Цуциева ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Normal Values ◽  
Kidney Injury ◽  
Control Group ◽  
Tubular Necrosis ◽  
Acute Rhabdomyolysis ◽  
Group Protein ◽  
Multipotent Mesenchymal Stem Cells ◽  
Therapeutic Transplantation

The aim of a given work was investigation of stem cell therapy for acute rhabdomyolysis-caused kidney injury in an experiment induced by intramuscular injection of 50% glycerol (0,8 ml per 100 g of body weight). Results of experiment showed that in experimental animals with therapeutic transplantation of mesenchymal stem cells (MSC) from the human placenta the glomerular filtration and tubular reabsorption was less decrease, than at control group. Protein excretion in experimental group was higher than normal values only on the 3rd day of experiment, in other time its was closed to normal values, unlike control group at which all time of experiment the excretion of protein was almost twice higher than normal values. Thus, transplantation of MSCs to the animal with toxic kidney injury effectively restores renal function in comparison.

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