scholarly journals Immunological purification of rat precartilaginous stem cells and constuction of the immortalized cell strain

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S153-S153
Author(s):  
Shuwei Zhang ◽  
Fengjing Guo ◽  
Hongbo You ◽  
Hui Liao ◽  
Xuebi Tian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Strain ◽  
Precartilaginous Stem Cells ◽  
Immortalized Cell

Immunological purification of rat precartilaginous stem cells and construction of the immortalized cell strain

2008 ◽  
Vol 128 (11) ◽  
pp. 1339-1344 ◽  
Author(s):  
Shuwei Zhang ◽  
Anmin Chen ◽  
Weihua Hu ◽  
Minghui Li ◽  
Hui Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Strain ◽  
Precartilaginous Stem Cells ◽  
Immortalized Cell

Mesenchymal stem cells immortalization's and state-of-art: a systematic review and meta-analysis

2021 ◽  
Vol 31 (Supplement_2) ◽  
Author(s):  
P E F Stricker ◽  
A C Irioda ◽  
B F Mogharbel ◽  
E Abdelwaid ◽  
L R Cavalli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Transfection ◽  
Meta Analysis ◽  
Total N ◽  
Htert Gene ◽  
Cell Immortalization ◽  
Immortalized Cell ◽  
Experimental Strategies ◽  
Established Technique

Abstract Background The immortalization of mesenchymal stem cells (MSCs) allows them to avoid senescence and be cultured through limitless cell passages. Thus, several experimental strategies, such as retrovirus-mediated gene transfer or viral oncogenesis, have been applied for the immortalization of MSCs. The aim was to identifier the most commonly used methodologies and their particularities for the immortalization of human and animal MSCs. Methods The search was conducted in June 2019 and developed in SCOPUS, PUBMED, and SCIENCE DIRECT. Statistical analysis was performed, obtaining the values of total n, mean and standard deviation, confidence interval (CI), and percentage (frequency) for all the predictors. Results The most used immortalization methodology was viral transfection, being the most common immortalized cell type was the bone marrow-derived MSC, and the most used gene for immortalizing both human and animal MSCs was hTERT (39.3%) and SV40T (54.5%). Among the articles analyzed in this review, only 39.3% and 36.4% of human and animal MSCs immortalization protocols, respectively, underwent the tumorigenicity test. Conclusions The virus-mediated gene transfection was observed as the most used and established technique. The insertion of the hTERT gene is still the most used gene for cell immortalization, suggesting that the maintenance of telomerase is efficient for maintaining cell proliferation and bypassing cell senescence. The review concluded that the tumorigenicity tests should become mandatory in order to safely use the immortalized MSCs for translation.

Effect of Astragaloside IV On Precartilaginous Stem Cells to Promote Bone Development in Rats

2021 ◽  
Author(s):  
Zhizhou Jiang ◽  
Hang Yin ◽  
Lei Zhao ◽  
Jianyong Jiang ◽  
Jinbo Ni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cultured Cells ◽  
Bone Development ◽  
Biological Characteristics ◽  
Control Group ◽  
Astragaloside Iv ◽  
Tibial Length ◽  
Antibody Staining ◽  
Precartilaginous Stem Cells ◽  
Experimental Group

Abstract Objective To explore the effect of astragaloside IV in promoting bone development by promoting the proliferation of precartilaginous stem cells. Methods To co-cultured the cells from the resting chondrocyte of growth plate and LaCroix of 24-hours old rats,and identified by FGFR-3 staining. Choosing astragaloside IV induce precartilaginous stem cells cultured in vitro, using Collagen type Ⅱ monoclonal antibody staining and MTT to test cell biological characteristics. Four 4 weeks old SD rats were selected and divided into an experimental group and control group, 24 rats in each group. The rats in the experimental group were injected with astragalus injection in a dose of 8.0g / kg once a day. The rats in the other group were injected with the same amount of normal saline. The 3rd and 5th week after feeding, 12 rats were killed, and the tibial length was measured by vernier caliper.Rusults The FGFR-3 staining was positive, which proved that the cultured cells were precartilaginous stem cells. Collagen typeⅡmonoclonal antibody staining is positive and the OD value detected by MTT test was higher, after astragaloside IV induced the precartilaginous stem cells. After astragaloside IV injection, the tibial length of experimental group measured by vernier caliper was significantly higher than that of the control group.Conclusion astragaloside IV can promote the proliferation and biological characteristics of precartilaginous stem cells, and then promote bone development.

scholarly journals Rosa26 docking sites for investigating genetic circuit silencing in stem cells

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Michael Fitzgerald ◽  
Mark Livingston ◽  
Chelsea Gibbs ◽  
Tara L Deans
Keyword(s):  
Stem Cells ◽  
Synthetic Biology ◽  
Cell Lines ◽  
Pluripotent Stem Cells ◽  
Genetic Circuit ◽  
Genetic Circuits ◽  
Immortalized Cell Lines ◽  
Site Specific Integration ◽  
Docking Sites ◽  
Immortalized Cell

Abstract Approaches in mammalian synthetic biology have transformed how cells can be programmed to have reliable and predictable behavior, however, the majority of mammalian synthetic biology has been accomplished using immortalized cell lines that are easy to grow and easy to transfect. Genetic circuits that integrate into the genome of these immortalized cell lines remain functional for many generations, often for the lifetime of the cells, yet when genetic circuits are integrated into the genome of stem cells gene silencing is observed within a few generations. To investigate the reactivation of silenced genetic circuits in stem cells, the Rosa26 locus of mouse pluripotent stem cells was modified to contain docking sites for site-specific integration of genetic circuits. We show that the silencing of genetic circuits can be reversed with the addition of sodium butyrate, a histone deacetylase inhibitor. These findings demonstrate an approach to reactivate the function of genetic circuits in pluripotent stem cells to ensure robust function over many generations. Altogether, this work introduces an approach to overcome the silencing of genetic circuits in pluripotent stem cells that may enable the use of genetic circuits in pluripotent stem cells for long-term function.

Immortalization of human precartilaginous stem cells by transfecting SV40Tag

2009 ◽  
Vol 29 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Junfang Wang ◽  
Huang Fang ◽  
Renyun Xia ◽  
Anming Chen ◽  
Hao Cheng
Keyword(s):  
Stem Cells ◽  
Precartilaginous Stem Cells

Fibroblast Growth Factor Receptor-3 as a Marker for Precartilaginous Stem Cells

1999 ◽  
Vol 367 ◽  
pp. S163-S175 ◽  
Author(s):  
Dror Robinson ◽  
Amir Hasharoni ◽  
Nir Cohen ◽  
Avner Yayon ◽  
Roland M. Moskowitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fibroblast Growth ◽  
Precartilaginous Stem Cells
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