scholarly journals Directed differentiation of human embryonic stem cells to neural progenitor cells and neurons on human feeder cells

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S146-S146
Author(s):  
Ding Yuan ◽  
Pingyuan Xie ◽  
Ge Lin ◽  
Guangxiu Lu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Directed Differentiation ◽  
Feeder Cells

scholarly journals SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells

iScience ◽  
2020 ◽  
Vol 23 (9) ◽  
pp. 101475
Author(s):  
Xinyuan Liu ◽  
Zhuoqing Fang ◽  
Jing Wen ◽  
Fan Tang ◽  
Bing Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor

scholarly journals Neural Progenitor Cells Derived from Human Embryonic Stem Cells as an Origin of Dopaminergic Neurons

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Parinya Noisa ◽  
Taneli Raivio ◽  
Wei Cui
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor

Human embryonic stem cells (hESCs) are able to proliferatein vitroindefinitely without losing their ability to differentiate into multiple cell types upon exposure to appropriate signals. Particularly, the ability of hESCs to differentiate into neuronal subtypes is fundamental to develop cell-based therapies for several neurodegenerative disorders, such as Alzheimer’s disease, Huntington’s disease, and Parkinson’s disease. In this study, we differentiated hESCs to dopaminergic neurons via an intermediate stage, neural progenitor cells (NPCs). hESCs were induced to neural progenitor cells by Dorsomorphin, a small molecule that inhibits BMP signalling. The resulting neural progenitor cells exhibited neural bipolarity with high expression of neural progenitor genes and possessed multipotential differentiation ability. CBF1 and bFGF responsiveness of these hES-NP cells suggested their similarity to embryonic neural progenitor cells. A substantial number of dopaminergic neurons were derived from hES-NP cells upon supplementation of FGF8 and SHH, key dopaminergic neuron inducers. Importantly, multiple markers of midbrain neurons were detected, includingNURR1, PITX3, andEN1, suggesting that hESC-derived dopaminergic neurons attained the midbrain identity. Altogether, this work underscored the generation of neural progenitor cells that retain the properties of embryonic neural progenitor cells. These cells will serve as an unlimited source for the derivation of dopaminergic neurons, which might be applicable for treating patients with Parkinson’s disease.

scholarly journals Differing Lectin Binding Profiles among Human Embryonic Stem Cells and Derivatives Aid in the Isolation of Neural Progenitor Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (8) ◽  
pp. e23266 ◽  
Author(s):  
Mahesh C. Dodla ◽  
Amber Young ◽  
Alison Venable ◽  
Kowser Hasneen ◽  
Raj R. Rao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Progenitor Cells ◽  
Lectin Binding ◽  
Embryonic Stem ◽  
Neural Progenitor

scholarly journals Transcriptional profiling of MEF2-regulated genes in human neural progenitor cells derived from embryonic stem cells

Genomics Data ◽  
2015 ◽  
Vol 3 ◽  
pp. 24-27 ◽  
Author(s):  
Shing Fai Chan ◽  
Xiayu Huang ◽  
Scott R. McKercher ◽  
Rameez Zaidi ◽  
Shu-ichi Okamoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Human Neural Progenitor Cells

Survival and differentiation of neural progenitor cells derived from embryonic stem cells and transplanted into ischemic brain

2005 ◽  
Vol 103 (2) ◽  
pp. 304-310 ◽  
Author(s):  
Yasushi Takagi ◽  
Masaki Nishimura ◽  
Asuka Morizane ◽  
Jun Takahashi ◽  
Kazuhiko Nozaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Ischemic Brain ◽  
Content Type ◽  
Fine Print

Object. Cell replacement therapy including the use of embryonic stem cells (ESCs) may represent a novel treatment for damage from stroke. In this study, the authors transplanted neural progenitor cells (NPCs) derived from ESCs into ischemic brain and analyzed their survival and differentiation. Methods. Multipotential NPCs were generated from ESCs by using the stromal cell—derived inducing activity method. These cells could differentiate in vitro into neurons, glia, and oligodendrocytes, thus revealing them to be neural stem cells. The NPCs were then transplanted into ischemic brain. At 2 weeks postischemia, the transplanted cells occupied 18.8 ± 2.5% of the hemispheric area; by 4 weeks postischemia, 26.5 ± 4% of the hemisphere. At 4 weeks after transplantation, green fluorescent protein (GFP)—positive transplanted cells showed mature neuronal morphological features. The authors also investigated the expression of differentiation markers and various neurotransmitters. Transplanted cells were immunopositive for neuronal nuclei, β-tubulin-III, and glial fibrillary acidic protein. Of the GFP-positive cells, 33.3 ± 11.5% were positive for glutamate decarboxylase, 13.3 ± 5.8% for glutamate, 2.1 ± 2.5% for tyrosine hydroxylase, 1.8 ± 2% for serotonin, and 0.4 ± 0.2% for choline acetyltransferase. Conclusions. The authors confirmed the survival and differentiation of ESC-derived NPCs transplanted into the ischemic brain. Surviving transplanted cells expressed several neural markers and neurotransmitters. These findings indicate that these cells can function in the brain.

Generation of Tripotent Neural Progenitor Cells from Rat Embryonic Stem Cells

2012 ◽  
Vol 39 (12) ◽  
pp. 643-651 ◽  
Author(s):  
Zhenkun Wang ◽  
Chao Sheng ◽  
Tianda Li ◽  
Fei Teng ◽  
Lisi Sang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor
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