scholarly journals Tumor progression in aberrant karyotypic human embryonic stem cells derived from long term suboptimal culture

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S123-S123
Author(s):  
Sheng Yang ◽  
Ge Lin ◽  
Guangxiu Lu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Tumor Progression ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

Tumor progression of culture-adapted human embryonic stem cells during long-term culture

2008 ◽  
Vol 47 (8) ◽  
pp. 665-679 ◽  
Author(s):  
Sheng Yang ◽  
Ge Lin ◽  
Yue-Qiu Tan ◽  
Di Zhou ◽  
Lei-Yu Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Tumor Progression ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Long Term Culture

Long-term, feeder-free maintenance of human embryonic stem cells by mussel-inspired adhesive heparin and collagen type I

2016 ◽  
Vol 32 ◽  
pp. 138-148 ◽  
Author(s):  
Mihyun Lee ◽  
Youngjin Kim ◽  
Ji Hyun Ryu ◽  
Kyuri Kim ◽  
Yong-Mahn Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Collagen Type ◽  
Embryonic Stem ◽  
Collagen Type I ◽  
Type I

Long-term culture of human embryonic stem cells in feeder-free conditions

2004 ◽  
Vol 229 (2) ◽  
pp. 259-274 ◽  
Author(s):  
Elen S. Rosler ◽  
Gregory J. Fisk ◽  
Ximena Ares ◽  
John Irving ◽  
Takumi Miura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Long Term Culture

Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells

2010 ◽  
Vol 28 (6) ◽  
pp. 606-610 ◽  
Author(s):  
Zara Melkoumian ◽  
Jennifer L Weber ◽  
David M Weber ◽  
Andrei G Fadeev ◽  
Yue Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Synthetic Peptide ◽  
Embryonic Stem ◽  
Cardiomyocyte Differentiation ◽  
Self Renewal

scholarly journals TPX2 Amplification-Driven Aberrant Mitosis in Long-Term Cultured Human Embryonic Stem Cells

2021 ◽  
Author(s):  
Ho-Chang Jeong ◽  
Young-Hyun Go ◽  
Joong-Gon Shin ◽  
Yun-Jeong Kim ◽  
Min-Guk Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mitotic Progression ◽  
Genome Wide ◽  
Hesc Culture ◽  
Spindle Stability

AbstractAlthough human embryonic stem cells (hESCs) are equipped with highly effective machinery for the maintenance of genome integrity, the frequency of genetic aberrations during long-term in vitro hESC culture has been a serious issue that raises concerns over their safety in future clinical applications. By passaging hESCs over a broad range of timepoints, we found that mitotic aberrations, such as the delay of mitosis, multipolar centrosomes, and chromosome mis-segregation, were increased in the late-passaged hESCs (LP-hESCs) in parallel with polyploidy compared to early-passaged hESCs (EP-hESCs). Through high-resolution genome-wide approaches and by following transcriptome analysis, we found that LP-hESCs with a minimal amplicon in chromosome 20q11.21 highly expressed TPX2 (targeting protein for Xklp2), a key protein for governing spindle assembly and cancer malignancy. Consistent with these findings, the inducible expression of TPX2 in EP-hESCs reproduced aberrant mitotic events, such as the delay of mitotic progression, spindle stability, misaligned chromosomes, and polyploidy. This data suggests that the amplification and increased transcription of the TPX2 gene at 20q11.21 could contribute to an increase in aberrant mitosis due to altered spindle dynamics.

Sign in / Sign up

Export Citation Format

Share Document