scholarly journals The fusion of somatic cells and human embryonic stem cells by electrofusion method

Cell Research ◽  
2008 ◽  
Vol 18 (S1) ◽  
pp. S57-S57 ◽  
Author(s):  
Lili Du ◽  
Jianjun Peng ◽  
Ge Lin ◽  
Guangxiu Lu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem

Human Embryonic Stem Cells May Display Higher Resistance to Genotoxic Stress as Compared to Primary Explanted Somatic Cells

2008 ◽  
Vol 17 (3) ◽  
pp. 599-608 ◽  
Author(s):  
Kumar Jayaseelan Vinoth ◽  
Boon Chin Heng ◽  
Anuradha Poonepalli ◽  
Birendranath Banerjee ◽  
Lakshmidevi Balakrishnan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Genotoxic Stress

scholarly journals Reprogramming By Cytosolic Extract of Human Embryonic Stem Cells Improves Dopaminergic Differentiation Potential of Human Adipose Tissue-Derived Stem Cells

2021 ◽  
Author(s):  
Sehar Mobasseri ◽  
◽  
Arash Javeri ◽  
Masoumeh Fakhr Taha ◽  
◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Somatic Cells ◽  
Embryonic Stem ◽  
Differentiation Potential ◽  
Cytoplasmic Extract ◽  
The Impact ◽  
Dopaminergic Differentiation

The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepared by repeated cycles of freezing and thawing. The plasma membrane of hADSCs was reversibly permeabilized by Streptolysin O (SLO), exposed to hESC extract and resealed by CaCl2-containing medium. As revealed by qPCR analysis, expression of OCT4, SOX2, NANOG, LIN28A and KLF4 mRNAs were downregulated in the ADSCs one week after extract incubation, while all except for KLF4 were upregulated at the end of second week. For dopaminergic differentiation, control and reprogrammed ADSCs were induced by serum-free neurobasal medium containing B27 and a cocktail of SHH, FGF8, bFGF and BDNF for 12 days. After differentiation, expression levels of some neuronal and dopaminergic-related genes, including PAX6, NESTIN, NEFL, GLI1, LMXB1, EN1, NURR1 and TH, were significantly increase in the reprogrammed ADSCs compared to the control group. As a whole, two weeks after reprogramming by ESC extract, ADSCs showed an improved dopaminergic differentiation potential. These findings suggest that the cytoplasmic extract of hESCs is containing some regulatory factors which induce the expression of pluripotency-associated markers in somatic cells and that the exposure to ESC extract may serve as a simple and rapid strategy to enhance plasticity of somaticstem cells for cell replacement therapy purposes.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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